(19) United States

US 20110166038A1

(19) United States (12) Patent Application Publication (10) Pub. No.: US 2011/0166038 A1 Zhang et al. (54)

(43) Pub. Date:

SCREENING METHODS FOR HEAT-SHOCK RESPONSE MODULATORS

(75) Inventors:

Jul. 7, 2011

Publication Classi?cation

(51)

Int. Cl.

Bin Shi Chung Zhang, Ng, SanSan Diego, Diego, CA CA (U S);

(

(US); Qingyan Au, Anaheim, CA

C07C 61/29

~

)

(2006,01)

(Us) (73)

Assignee:

SCREENING METHODS FOR HEAT-SHOCK RESPONSE MODULATIORS

(21) Appl' NO‘: .

(22) 86

12/994’703 _

PCT Flled' PCT N ‘I

( )

52

)

US. Cl. ............................ .. 506/10" 506/33‘ 562/403

’

’

(57) ABSTRACT High-throughput methods are provided for quantitatively measuring the modulation of heat shock protein (HSP)

Jun' 3’ 2009 PCT[U509 /03 401

0

expression in a cell by exposing the cell to at least one stress and measuring cellular stress responses. High-throughput

methods for identifying modulators (activators or inhibitors)

§ 371 (6X1)

(2) (4) Date’:

ofHSP or HSF expression in a cell by treating the cell With an

Jan_ 21, 2011

’

(30)

(

agent, such as a compound or composition, exposing the cell to a stress, and measuring responses of the cell to the stress in

Foreign Application Priority Data

the presence or absence of the agent are also provided. Devices useful in performing high-throughput methods of the

Jun. 3, 2008

(US) ................................. .. 61/130945

invention, and modulators identi?ed using Such methods, are

Oct. 10, 2008

(US) ................................. .. 61/194984

also provided

Patent Application Publication

Jul. 7, 2011

Sheet 1 0f 45

US 2011/0166038 A1

Patent Application Publication

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Jul. 7, 2011

Sheet 2 0f 45

US 2011/0166038 A1

Patent Application Publication

Jul. 7, 2011

Sheet 3 0f 45

US 2011/0166038 A1

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