Data Sheet HUMAN BONE SIALOPROTEIN (BSP)


Nov 9, 2016 - Matrix: Purified IgY; PBS pH 7.4. Specificity: Human Bone Sialoprotein. There was no cross reactivity obtained with human. Osteonectin, human ...

Data Sheet HUMAN BONE SIALOPROTEIN (BSP) ANTIBODY, POLYCLONAL Catalog no.:

A 4221.1 / A 4221.2

Immunogen:

Human Bone Sialoprotein (bone extract)

Synonyms:

Bone sialoprotein 2, BSP2, Cell-binding sialoprotein, Integrin-binding sialoprotein

Swiss-Prot No:

P21815

Gene Information:

Gene Name: IBSP Gene ID: 3381

Host:

Chicken

Matrix:

Purified IgY; PBS pH 7.4

Specificity:

Human Bone Sialoprotein There was no cross reactivity obtained with human Osteonectin, human Osteopontin, and human Alkaline Phosphatase.

Contents:

10 µg / 100 µg (lyophilized) Resuspend in 10 µl / 100 µl aqua bidest.

Known applications:

ELISA (1 μg/ml), RIA (20 ng/ml)1, 4, flow cytometry2, Western Blot (1:100)1, 3, immunohistochemistry (paraffin sections, 4 μg/ml)4 This antibody has not been tested for use in all applications. This does not necessarily exclude its use in non-tested procedures. The stated dilutions are recommendations only. End users should determine optimal dilutions in their system using appropriate negative/positive controls.

Store at:

2-8 °C (lyophilized); - 20 °C (dissolved) Repeated thawing and freezing must be avoided

References:

1. Karmatschek M, Maier I, Seibel MJ, Woitge HW, Ziegler R, Armbruster FP (1997). Improved purification of human bone sialoprotein and development of a homologous radioimmunoassay. Clin Chem 43:2076-82. 2. Bellahcene A, Van Riet, I, de Greef C, Antoine N, Young MF, Van Camp B, Castronovo V (2000). Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients. Br J Haematol 111:1118-21. 3. Adwan H, Bauerle T, Najajreh Y, Elazer V, Golomb G, Berger MR (2004). Decreased levels of osteopontin and bone sialoprotein II are correlated with reduced proliferation, colony formation, and migration of GFP-MDA-MB-231 cells.Int J Oncol. 24(5):1235-44. 4. Kayed H, Kleeff J, Keleg S, Felix K, Giese T, Berger MR, Buchler MW, Friess H (2007). Effects of bone sialoprotein on pancreatic cancer cell growth, invasion and metastasis. Cancer Lett 245(1-2): 171183.

Last updated on:

09 November 2016

For research use only Publishing research using A 4221? Please let us know so that we can cite your publication as a reference.

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