SUPPORTING INFORMATION Contents:
Micropatterned multicolor dynamically adhesive substrates to control cell adhesion and multicellular organization
Natalia M. Rodriguez1,2, Ravi A. Desai1, Britta Trappmann1,2, Brendon M. Baker1,2, Christopher S. Chen1,2,*
1
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA
2
Department of Biomedical Engineering, Boston University, Boston, MA, USA and
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA * to whom correspondence should be addressed ([email protected])
SUPPORTING INFORMATION
Contents: Figure S1. Neutravidin coating requires Pluronics to repel cell adhesion. Figure S2. Neutravidin-Pluronics surface coating stability.
1
Figure S1. Neutravidin coating requires Pluronics to repel cell adhesion. Human Umbilical Vein Endothelial Cells (HUVECs) were seeded on micro patterned fibronectin and Neutravidin substrates with or without Pluronic F127 (Figure 1a, panel i, step 3; and Figure 1a, panel ii, step 4), and imaged approximately 24h later. Scale bars, 25 microns.
2
Figure S2. Neutravidin-Pluronics surface coating stability. Human Umbilical Vein Endothelial Cells (HUVECs) were seeded onto micropatterned substrates that consisted of 140um x 140um fibronectin islands surrounded by Pluronics F127 (top row) or Neutravidin+Pluronics F127 (bottom row). Images were acquired at indicated timepoints, and suggest that fibronectin-Neutravidin/Pluronics substrates are as stable as fibronectinPluronics substrates for 2 days. Scale bars, 150 microns.
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Natalia M. Rodriguez1,2, Ravi A. Desai1, Britta Trappmann1,2, Brendon M. Baker1,2, Christopher S. Chen1,2,*
1
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA
2
Department of Biomedical Engineering, Boston University, Boston, MA, USA and
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA * to whom correspondence should be addressed ([email protected])
SUPPORTING INFORMATION
Contents: Figure S1. Neutravidin coating requires Pluronics to repel cell adhesion. Figure S2. Neutravidin-Pluronics surface coating stability.
1
Figure S1. Neutravidin coating requires Pluronics to repel cell adhesion. Human Umbilical Vein Endothelial Cells (HUVECs) were seeded on micro patterned fibronectin and Neutravidin substrates with or without Pluronic F127 (Figure 1a, panel i, step 3; and Figure 1a, panel ii, step 4), and imaged approximately 24h later. Scale bars, 25 microns.
2
Figure S2. Neutravidin-Pluronics surface coating stability. Human Umbilical Vein Endothelial Cells (HUVECs) were seeded onto micropatterned substrates that consisted of 140um x 140um fibronectin islands surrounded by Pluronics F127 (top row) or Neutravidin+Pluronics F127 (bottom row). Images were acquired at indicated timepoints, and suggest that fibronectin-Neutravidin/Pluronics substrates are as stable as fibronectinPluronics substrates for 2 days. Scale bars, 150 microns.
3
