1 Supplementary figure legend 1 2 Supplementary table 1 ... - JCI

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Supplementary figure legend

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Supplementary table 1. Sequence of sense and antisense primers use in RT-QPCR experiments.

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Supplementary table S2. Expression of IL-1β receptor mRNA.

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Supplementary Figure 1. Y. pseudotuberculosis infected does not increase the number of

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apoptotic in Peyer’s patches.

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PP from WT mice were mounted in Ussing chamber and incubated or not with Yersinia pIB102

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strain at 1.107cfu/mL. Then, the number of apoptotic cells identified by caspase-3 immunostaining

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was counted inside dome and FAE from PP. 2 hours of incubation with the Yersinia pIB102 strain

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did not modify the number of apoptotic cells inside the dome and FAE of PP. Enlargements of the

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fields delineated by the rectangles are shown in A’ and B’ (Bars: 250 µm).

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Supplementary Figure 2. TLR-2 and/or TLR-4 activation increases IL-1β expression.

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106 THP-1 cells were infected with Yersinia pIB102 strain (MOI10) or stimulated with Pam4CSK3

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(20µg/mL) and/or LPS (20µg/mL) from E. coli for 2 hours. Then THP-1 cells were centrifuged and

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the IL-1β level in the supernatant was measured by ELISA. (at least n=8 per group; mean±s.e.m;

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