70x100 cm vertical poster template
ARE THE BIOMIMETIC THERAPIES PROMISING FOR DIRECT PULP CAPPING? Elif Aybala OKTAY¹, Ülkü ÖZAN², Fulya TOKSOY TOPÇU¹, Hüseyin TORT¹ ¹ Gülhane Military Medical Academy, Department of Restorative Dentistry , Ankara, TURKEY ² Abant İzzet Baysal University,Deparment of Endodontics, Bolu, Turkey
Aim
To examine the cytotoxic effects of Emdogain (EMD) a purified acidic biomaterial containing amelogenins which are obtained from pig in the embryological term and which are enamel matrix derivatives out of dentin bonding agents as pulp capping in the dental pulp therapies with RTCA XCELLigence impedance method.
Material - Method
The cytotoxic effects of four different dentin agents (Tokuyama Bond Force, Shofu Beauti Bond, Dentsply Prime&Bond NT, 3M Adper Single Bond) used as pulp capping material in the direct pulp therapies and Emdogain (EMD), a enamel matrix derivative, and a purified acidic biomaterial containing amelogenins obtained from pig in the embryological term on the dental pulp cells, with RTCA XCELLigence impedance method were analyzed. Dental Pulp cell cultures were used between the third to sixth passage only in this study. Dental Pulp cell cultures were transferred to the wells of x celligence to be used in the subsequent phases of this study. It was evaluated with real time cellular impedance method whether or not different doses of DBA’s added in media caused effects upon dental pulp in vitro. 1/250, 1/500 and 1/1000 dilutions of all medications were used.
RTCA Analyzer RTCA Control Unit
RTCA Plate
Result We found out that all doses of EMD decreased the cell growth based upon the days exposed and it showed no cytotoxic effect. Our results showed that some dentin bonding agents have cytotoxic effects on dental pulp cells and they may inhibit dental pulp regeneration by decreasing cell proliferation.
Conclusion It can be concluded that Emdogain is a material which can be preferred for pulp capping against dentin bonding agents.
Bonding Agents
Emdogain
www.postersession.com
Aim
To examine the cytotoxic effects of Emdogain (EMD) a purified acidic biomaterial containing amelogenins which are obtained from pig in the embryological term and which are enamel matrix derivatives out of dentin bonding agents as pulp capping in the dental pulp therapies with RTCA XCELLigence impedance method.
Material - Method
The cytotoxic effects of four different dentin agents (Tokuyama Bond Force, Shofu Beauti Bond, Dentsply Prime&Bond NT, 3M Adper Single Bond) used as pulp capping material in the direct pulp therapies and Emdogain (EMD), a enamel matrix derivative, and a purified acidic biomaterial containing amelogenins obtained from pig in the embryological term on the dental pulp cells, with RTCA XCELLigence impedance method were analyzed. Dental Pulp cell cultures were used between the third to sixth passage only in this study. Dental Pulp cell cultures were transferred to the wells of x celligence to be used in the subsequent phases of this study. It was evaluated with real time cellular impedance method whether or not different doses of DBA’s added in media caused effects upon dental pulp in vitro. 1/250, 1/500 and 1/1000 dilutions of all medications were used.
RTCA Analyzer RTCA Control Unit
RTCA Plate
Result We found out that all doses of EMD decreased the cell growth based upon the days exposed and it showed no cytotoxic effect. Our results showed that some dentin bonding agents have cytotoxic effects on dental pulp cells and they may inhibit dental pulp regeneration by decreasing cell proliferation.
Conclusion It can be concluded that Emdogain is a material which can be preferred for pulp capping against dentin bonding agents.
Bonding Agents
Emdogain
www.postersession.com
