Agilent 1200 Series UV-visible detectors
Agilent 1200 Series UV-visible Detectors
Six detectors to meet all your requirements The Agilent 1200 Series LC offers the UV-visible detector that best suits your needs, from programmable single wavelength to high speed multiwavelength and full spectral detection. • 1200 Series variable wavelength detector (VWD) Lowers detection limits with a simplified design, optimized for high sensitivity and low baseline drift.
• 1200 Series multiple wavelength detector (MWD) Allows simultaneous detection of up to eight different wavelengths without sacrificing sensitivity.
VWD SL
MWD
MWD SL
DAD
DAD SL 1200 DAD SL
1200 DAD For high
1200 MWD SL sensitivity in 1200 MWD
For high sensitivity in ultra-fast, multi-λ analysis
multi-λ and spectral analysis
For high sensitivity in ultra-fast, multi-λ and spectral analysis
• 8 signals • 8 signals • 80-Hz data rate • 20-Hz data rate • Full spectra, For maximum multi-λ analysis • 8 signals 1200 VWD • Full spectra, 80 Hz sensitivity in • 8 signals • 80-Hz data rate 20 Hz • DRC and RFID* For highly ultra-fast • 20-Hz data rate • DRC and RFID* • RFID* sensitive single-λ analysis • RFID* single-λ analysis • 1 signal • 1 signal • 55-Hz data rate • 13-Hz data rate For high
1200 VWD SL sensitivity in
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The Agilent 1200 Series multiple wavelength and diode array detectors with new low noise flow cells and electronics provide maximum sensitivity and baseline stability. Patented radio frequency identification (RFID) tracking technology for flow cells and UV lamp offers a new level of data traceability.
VWD
Ne w
• 1200 Series variable wavelength detector SL (VWD SL) Enables high-speed UV detection with up to 55-Hz sampling rate for high performance in ultra-fast LC applications.
Performance & Functionality *DRC – Data Recovery Card, RFID – Radio Frequency Identification tags for lamp and flow cell
• 1200 Series multi-wavelength detector SL (MWD SL) Permits detection of up to 8 signals with up to 80-Hz sampling rate, providing uncompromised compatibility with the analysis speed delivered by ultra-fast LC. Embedded data recovery gives unique “data-never-lost insurance”.
• 1200 Series diode array detector (DAD) Provides full power of multiple wavelength detection and spectral information with high resolution and sensitivity.
• 1200 Series diode array detector SL (DAD SL) Detects full spectra and 8 signals with up to 80-Hz sampling rate for ultimate confidence in ultra-fast LC. New data recovery card and RFID technology provide new levels of data security and traceability.
Excellent performance and functionality for a wide range of needs Agilent 1200 Series UV-visible detectors are all designed with a broad range of features to achieve high sensitivity and outstanding productivity: • State-of-the-art optics, electronics and flow cell design provide maximum sensitivity and baseline stability, even under demanding gradient conditions. • A wide range of flow cells enables optimized detector performance for all application needs from nanoflow to preparative LC.
• The 1200 Series instant pilot provides software-independent method and sequence setup, run control and signal display for convenient standalone instrument control. • Broad range of software solutions, including the new Agilent ChemStation, and Agilent EZChrom Elite, provide easy and convenient instrument control, data handling and evaluation, reporting and diagnostics. • Monitoring of multiple signals and spectral data enables immediate review of the separation process results. • Built-in GLP/GMP compliance features include automated wavelength verification, tracking of system performance and maintenance. New data recovery card (DRC) and radio frequency identification RFID technology provide a new level of data security and traceability.
• Checks with preset chromatographic control criteria implement automated performance qualification (PQ). A built-in system suitability software alerts the user and, if necessary, stops the system. • Early maintenance feedback (EMF) issues timely maintenance warnings at predefined limits to keep the system operating at maximum productivity and minimum cost. • Front access to lamps and flow cells enables fast and convenient maintenance for maximum uptime.
Selection of up to eight wavelength signals on the DAD and MWD provides for increased sensitivity and selectivity for medium-complex samples. The latest version of Agilent ChemStation facilitates convenient and fast review of results from large numbers of samples.
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Agilent 1200 Series Variable Wavelength Detector High sensitivity and stability The Agilent 1200 Series VWD provides high sensitivity and improves productivity in routine work: • Minimum baseline noise and maximum baseline stability for lowest detection limits • A wide linear range for reliable, simultaneous quantitation of primary compounds, by-products and impurities • Programmable wavelength switching for sensitivity and selectivity optimized for each analyte’s elution • Stop-flow wavelength scanning for quick wavelength optimization • Automatic wavelength verification by built-in holmium oxide filter
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Flow cells: • Standard
Double beam Deuterium lamp 1 13 Hz ± 0.75 x 10-5 AU at 254 nm 3 x 10-4 AU/h at 254 nm > 2 AU upper limit 190 – 600 nm ± 1 nm, self-calibration with deuterium lines, verification with Holmium oxide filter 6.5 nm typical 14-µL volume, 10-mm cell path length 40 bar (588 psi) maximum pressure 5-µL volume, 6-mm cell path length 40 bar (588 psi) maximum pressure 1-µL volume, 5-mm cell path length 40 bar (588 psi) maximum pressure 14-µL volume, 10-mm cell path length 400 bar (5880 psi) maximum pressure Wavelength, polarity, peak width, lamp on/off Stop-flow wavelength scan Recorder/Integrator 100 mV or 1 V, 1 output Cuvette holder
• Semi-micro • Micro • High pressure Time programmable: Spectral tools: Analog output: Optional: *According to ASTM E1657-98
Redesigned optics provide high signal level, low baseline noise, low drift and minimum refractive index effects.
The Holmium oxide filter swings into the light path to verify that the wavelength matches the setpoints.
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Agilent 1200 Series Variable Wavelength Detector SL Maximum sensitivity in ultra-fast LC The Agilent 1200 Series VWD SL provides highly sensitive single wavelength analysis in fast and ultra-fast LC applications with peak widths narrower than 1 second. This detector offers: • Sampling rate up to 55 Hz • Minimum baseline noise and maximum baseline stability for lowest detection limits • A wide linear range for reliable, simultaneous quantitation of primary compounds, by-products, and impurities • Programmable wavelength switching for sensitivity and selectivity optimized for each analyte’s elution • Stop-flow wavelength scanning for quick wavelength optimization • Automatic wavelength verification by built-in holmium oxide filter
Specifications Detector type: Light source: Number of signals: Maximum sampling rate Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Flow cells: • Standard
Double beam Deuterium lamp 1 55 Hz ± 0.75 x 10-5 AU at 254 nm 3 x 10-4 AU/h at 254 nm > 2 AU upper limit 190 – 600 nm ± 1 nm, self-calibration with deuterium lines, verification with Holmium oxide filter 6.5 nm typical 14-µL volume, 10-mm cell path length 40 bar (588 psi) maximum pressure 5-µL volume, 6-mm cell path length 40 bar (588 psi) maximum pressure 1-µL volume, 5-mm cell path length 40 bar (588 psi) maximum pressure 14-µL volume, 10-mm cell path length 400 bar (5880 psi) maximum pressure Wavelength, polarity, peak width, lamp on/off Stop-flow wavelength scan Recorder/Integrator 100 mV or 1 V, 1 output Cuvette holder
• Semi-micro • Micro • High pressure Time programmable : Spectral tools: Analog output: Optional: *According to ASTM E1657-98
Using ultra-fast LC conditions with increased data rate significantly improves resolution.
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Agilent 1200 Series Multiple Wavelength Detector More information and flexibility without loss of sensitivity
The Agilent 1200 Series MWD is a dedicated multiple wavelength detector that offers the same high sensitivity for both single and multiple wavelength detection based on a diode array design with a wavelength range from 190 to 950 nm. • Simultaenous acquisition of up to 8 compound-specific wavelenghts for increased sensitivity and selectivity. • Electronic Temperature Control (ETC) provides maximum baseline stability and practical sensitivity under conditions of fluctuating ambient temperature and humidity. • Wide linear range for reliable quantitation of by-products and impurities, down to levels of < 0.05 % of the primary compound. • Low noise front-end electronics and new, patented flow cell design delivers lowest detection limits resulting from minimized short-term noise (typical < ± 6 µAU ASTM). • Programmable slit for rapid optimization of sensitivity, linearity and spectral resolution. • Range of 9 flow cells provide maximum application flexibility.
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Diode width: Wavelength bunching: Flow cells: • Standard • Semi-micro • Micro • Semi-nano • Nano • High pressure • Preparative • Preparative • Preparative Time programmable: Analog output:
1024-element diode array Deuterium and tungsten lamp 8 20 Hz ± 0.8 x 10-5 AU at 254 nm and at 750 nm < 0.9 x 10-3 AU/h at 254 nm > 2 AU upper limit 190 – 950 nm ± 1 nm, self-calibration with deuterium, lines verification with Holmium oxide filter Programmable: 1, 2, 4, 8, 16 nm < 1 nm Programmable: 2 to 400 nm, in steps of 1 nm 13-µL volume, 10-mm path length, 120 bar (1760 psi) 5-µL volume, 6-mm path length, 120 bar (1760 psi) 2-µL volume, 3-mm path length, 120 bar (1760 psi) 500-nL volume, 10-mm path length, 50 bar (733 psi) 80-nL volume, 6-mm path length, 50 bar 1.7-µL volume, 6-mm path length, 400 bar 3-mm path length, 120 bar (1760 psi) 0.3-mm path length, 20 bar (294 psi) 0.06-mm path length, 20 bar (294 psi) Wavelength, polarity, peak width, on/off lamp on/off bandwidth, autobalance Recorder/Integrator 100 mV or 1 V, 2 outputs
*According to ASTM E1657-98
• Prepared for the future by upgradeability to full spectral capabilities and/or 80 Hz sampling rate. • RFID tags for all flow cells and UV lamp provide new levels of data traceability by
recording parameters, such as product and serial number, production date, cell dimensions, lamp usage, and the last date of a successful cell or lamp test.
mAU 3000
Ginko Biloba extract Active compounds Quercetin
2500
Kaempferol
2000
204 nm
Isorhamnetin
1500 1000
370 nm 500 254 nm 0 2
4
6
8
10
12
14
16
18 Time [min]
Sensitive quantitation of active compounds and impurities in a single run without wavelength switching.
A long-life (> 2000 hours) deuterium lamp reduces operating costs.
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Agilent 1200 Series Multiple Wavelength Detector SL High sensitivity and more information for ultra-fast LC Ultra-fast separations can generate sharp and narrow peaks with peak widths smaller than 1 second. The Agilent 1200 Series multiple wavelength detector SL (MWD SL) offers multiple wavelength detection of 8 signals at up to 80 Hz sampling rate, keeping up with the analysis speed of ultra-fast LC. Even when operated at high data rates, the MWD SL allows precise quantification at trace levels. This exceptional capability assures that your ultra-fast LC methods comply with the strictest regulatory performance requirements. • Diode array design enables simultaneous acquisition of up to 8 compound-specific wavelengths for increased sensitivity and selectivity. • Programmable slit for rapid optimization of sensitivity, linearity and spectral resolution. • Low noise front-end electronics and new, patented flow cell design delivers lowest detection limits resulting from minimized short-term noise (typical < ± 6 µAU ASTM). • Wide linear range for reliable quantification of impurities, down to levels of ,0.05 % for the primary compound. • Electronic Temperature Control (ETC) provides maximum baseline stability and practical sensitivity under conditions of fluctuating ambient temperature and humidity.
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Diode width: Wavelength bunching: Flow cells: • Standard • Semi-micro • Micro • Semi-nano • Nano • High pressure • Preparative • Preparative • Preparative Time programmable: Analog output:
1024-element diode array Deuterium and tungsten lamp 8 80 Hz < ± 0.8 x 10-5 AU at 254 nm and at 750 nm < 0.9 x 10-3 AU/h at 254 nm > 2 AU upper limit 190 – 950 nm ± 1 nm, self-calibration with deuterium, lines verification with Holmium oxide filter Programmable: 1, 2, 4, 8, 16 nm < 1 nm Programmable: 2 to 400 nm, in steps of 1 nm 13-µL, 10-mm path length, 120 bar (1760 psi) 5-µL, 6-mm path length, 120 bar (1760 psi) 2-µL, 3-mm path length, 120 bar (1760 psi) 500-nL, 10-mm path length, 50 bar (733 psi) 80-nL, 6-mm path length, 50 bar 1.7-µL, 6-mm path length, 400 bar 3-mm path length, 120 bar (1760 psi) 0.3-mm path length, 20 bar (294 psi) 0.06-mm path length, 20 bar (294 psi) Wavelength, polarity, peak width, on/off lamp, on/off bandwidth, autobalance Recorder/Integrator 100 mV or 1 V, 2 outputs
*According to ASTM E1657-98
• “Data-never-lost insurance” with built-in Data Recovery Card (DRC) prevents data losses in the event of communication breakdowns between instrument and PC. Raw data and meta data are automatically buffered on an embedded memory card. • RFID tags for all flow cells and UV lamp provide new levels of data traceability by recording parameters,
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such as product and serial number, production date, cell dimensions, lamp usage, and the last date of a successful cell or lamp test. This information is saved with each individual raw data file for unambiguous data traceability, minimizing the risk of false data interpretation. • Range of 9 analytical and preparative flow cells provide maximum application flexibility,
Short-term ASTM noise of the Agilent 1200 Series MWD/ DAD under different conditions Typical noise is well below the specified 16 µAU peak-to-peak under ASTM conditions (4-nm slit, time constant 1 s time, 2.5-Hz data rate). Lowest noise, 4 µAU, is obtained with 2.5 Hz and the 16-nm slit, however, even at 80 Hz and 4-nm slit the noise evel is still sufficient to quantify impurities at 0.05 % level with a signal/noise > 20. This complies with the strictest regulatory requirements. Under ultra-fast LC gradient conditions, baseline noise may increase somewhat. In such cases, a 40-Hz data rate and/or 8 or 16-nm slit can be used to reduce noise and increase sensitivity.
Limit of detection for anthraceneunder ultra-fast LC conditions as low as 300 fg – 10 x faster than in conventional LC The new Agilent 1200 Series MWD SL and DAD SL provide the same or lower limits of detection under ultra-fast conditions as the Agilent 1100 Series MWD and DAD under conventional LC conditions. For a data rate of 10 Hz, the LOD is up to 50 % lower compared to 1100 Series DAD and MWD measured at 2.5 Hz.
LOD of Anthracene under ultra-fast conditions
Data Rate: 80 Hz, LOD = 2.4 pg
Data Rate: 10 Hz, LOD = 0.6 pg
Compare with conventional HPLC LOD/pg on 1100 Binary/DAD “B“
Retention time = 2 min, Peak width = 6 s
LOD/pg in conventional LC on 1100 DAD/MWD “B” Conditions: Conventional LC using 1100 DAD/MWD “B”
Baseline wander of the 1200 MWD/DAD under harsh environmental conditions The new electronic temperature control (ETC) of the 1200 Series MWD/DAD reduces baseline wander by more than a factor of 20. The example demonstrates that, even under the harshest ambient conditions, the baseline of the Agilent 1200 Series DAD remains constant within 30 µAU/°C.
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Agilent 1200 Series Diode Array Detector Complete confidence in spectral data The Agilent 1200 Series diode array detector allows both quantification and confirmation of the analyte identity by spectral libraries at lowest trace level amounts in a single analysis. Verify separation quality with peak purity analysis interactively or automatically in the same run as your analytical quantification. • Deuterium and tungsten lamps with a wavelength range of 190 to 950 nm offer an expanded detection range with maximum light intensity for lowest minimum detection limits. • 1024 diodes and a 1-nm slit achieve high spectral resolution. • Programmable slit (1 to 16 nm) for rapid optimization of sensitivity, linearity and spectral resolution. • Low noise front-end electronics and patented flow cell design delivers lowest detection limits resulting from minimized short-term noise (typical < ± 6 µAU ASTM). • Diode array design enables simultaneous acquisition of up to 8 compound-specific wavelengths for increased sensitivity and selectivity. • Electronic temperature control (ETC) provides maximum baseline stability and practical sensitivity under fluctuating ambient temperature and humidity conditions.
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Diode width: Wavelength bunching: Flow cells: • Standard • Semi-micro • Micro • Semi-nano • Nano • High pressure • Preparative • Preparative • Preparative Time programmable:
• Prepared for the future by upgradeability to 80 Hz sampling rate for ultra-fast separations.
13-µL volume, 10-mm path length, 120 bar (1760 psi) 5-µL volume, 6-mm path length, 120 bar (1760 psi) 2-µL volume, 3-mm path length, 120 bar (1760 psi) 500-nL volume, 10-mm path length, 50 bar (733 psi) 80-nL volume, 6-mm path length, 50 bar 1.7-µL volume, 6-mm path length, 400 bar 3-mm path length, 120 bar (1760 psi) 0.3-mm path length, 20 bar (294 psi) 0.06-mm path length, 20 bar (294 psi) Wavelength, polarity, peak width, on/off lamp, on/off bandwidth, autobalance Data analysis software for spectra evaluation, including spectral libraries and peak purity functions Recorder/Integrator 100 mV or 1 V, 2 outputs
Spectral tools: Analog output: *According to ASTM E1657-98
Absorbance [normalized]
1 nm slit 0.6 0.5 0.4 0.3 0.2 0.1 0
Benzo(g,h,i)perylene Standard, 725 mAUFS
0.7 mAU
DAD 1, 21.801 Sample, 1.1 mAUFS Match factor: 988 230 240 250 260 270 280 nm
• RFID tags for all flow cells and UV lamp provide new levels of data traceability by recording parameters, such as product and serial number, production date, cell dimensions, lamp usage, and the last date of a successful cell or lamp test. • Range of 9 analytical and preparative flow cells provide maximum application flexibility,
1024-element diode array Deuterium and tungsten lamp 8 20 Hz (both spectra and signals) ± 0.8 x 10-5 AU at 254 nm and at 750 nm 0.9 x 10-3 AU/h at 254 nm > 2 AU upper limit 190 – 950 nm ± 1 nm, self-calibration with deuterium, lines verification with Holmium oxide filter Programmable: 1, 2, 4, 8, 16 nm < 1 nm Programmable: 2 to 400 nm, in steps of 1 nm
1 nm
2 nm
4 nm
240 260 8 nm
280 300 320
340
360 380
Wavelength [nm]
Quantitative and qualitative information in trace analysis. Flexible slit setting from fine bands to highest sensitivity. At a setting of 1 nm, and even 0.7 mAUFS, the fine structure of benzene can be revealed. A broad 8-nm or 16-nm slit minimizes baseline noise for enhanced sensitivity.
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Agilent 1200 Series Diode Array Detector SL High sensitivity and confidence in ultra-fast LC Ultra fast separations can generate sharp and narrow peaks with peak widths smaller than 1 second. The Agilent 1200 Series DAD SL offers multiple wavelength and full spectral detection at up to 80 Hz sampling rate, keeping up with the analysis speed of ultra-fast LC. Even when operated at high data rates, the 1200 Series DAD SL allows precise identification, quantification and peak purity analysis at trace levels. This exceptional capability assures that your ultra-fast LC methods comply with the strictest regulatory performance requirements.
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Diode width: Wavelength bunching: Flow cells: • Standard • Semi-micro • Micro • Semi-nano • Nano • High pressure • Preparative • Preparative • Preparative Time programmable: Spectral tools: Analog output:
1024-element diode array Deuterium and tungsten lamp 8 80 Hz (both spectra and signals) < ± 0.8 x 10-5 AU at 254 nm and at 750 nm < 0.9 x 10-3 AU/h at 254 nm > 2 AU upper limit 190 – 950 nm ± 1 nm, self-calibration with deuterium, lines verification with Holmium oxide filter Programmable: 1, 2, 4, 8, 16 nm < 1 nm Programmable: 2 to 400 nm, in steps of 1 nm 13-µL, 10-mm path length, 120 bar (1760 psi) 5-µL, 6-mm path length, 120 bar (1760 psi) 2-µL, 3-mm path length, 120 bar (1760 psi) 500-nL, 10-mm path length, 50 bar (733 psi) 80-nL, 6-mm path length, 50 bar 1.7-µL, 6-mm path length, 400 bar 3-mm path length, 120 bar (1760 psi) 0.3-mm path length, 20 bar (294 psi) 0.06-mm path length, 20 bar (294 psi) Wavelength, polarity, peak width, on/off lamp, on/off bandwidth, autobalance Data analysis software for spectra evaluation, including spectral libraries and peak purity functions Recorder/Integrator 100 mV or 1 V, 2 outputs
*According to ASTM E1657-98
80-Hz full spectral sampling rate provides resolution and peak capacity gains of 90 % (30 %) relative to 10 Hz (20 Hz) sampling rates.
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• Full spectral data acquisition rate of 80 Hz increases resolution by up to 100 % and delivers ultra-fast, highly sensitive peak purity analysis and spectral conformation, even at trace levels. • Dual lamp design for highest sensitivity from 190 nm to 950 nm. • Diode array design enables simultaneous acquisition of up to 8 compound-specific wavelengths for increased sensitivity and selectivity. • Programmable slit (1 to 16 nm) for rapid optimization of sensitivity, linearity and spectral resolution provides optimum incident light conditions.
• Low noise front-end electronics and patented flow cell design delivers lowest detection limits resulting from minimized short-term noise (typical < ± 6 µAU ASTM). • Electronic temperature control (ETC) provides maximum baseline stability and practical sensitivity under fluctuating ambient temperature and humidity conditions. • “Data-never-lost insurance” with built-in data recovery card (DRC) prevents data losses in the event of communication breakdowns between instrument and PC by automatically buffering raw data and meta data on an embedded memory card.
• RFID tags for all flow cells and UV lamp provide new levels of data traceability by recording parameters, such as product and serial number, production date, cell dimensions, lamp usage, and the date of the last successful cell or lamp test. This information is saved with each individual raw data file for unambiguous data traceability, minimizing the risk of false data interpretation. • Range of 9 analytical and preparative flow cells provide maximum flexibility, compatibility and investment protection.
• Reference wavelength eliminates background interference.
Under ultra-fast conditions, the DAD/MWD SL allows simultaneous identification and quantification of primary compounds and impurities at 0.03 % level – in a single run with the same or better precision and signal/noise provided by conventional LC.
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Peak purity analysis of trace level compounds under ultra-fast LC conditions The 1200 DAD SL enables peak purity analysis under ultra-fast LC conditions, even for trace level compounds at < 0.1 % level. In this case, the spectral analysis confirms that the nifedipin peak as identified by chromatographic retention was pure. This positive purity confirmation significantly increases confidence in quantitative chromatographic results. The exceptional capability of accurate spectral analysis at trace levels is enabled by the dual lamp design of the Agilent 1200 Series DAD SL. It provides lowest noise over the complete wavelength range for significantly increased spectral quality.
Spectral conformation of trace level compounds at < 0.1 % level under ultra-fast LC conditions A library search is executed for the measured trace level spectrum. Match factors are calculated and tabulated in the Library Search Result table. Highest spectral match is achieved for nifedipin with a match factor of 962 compared to 929 for the primary compound nimodipin, which shows high spectral similarity to nifedipin. The spectral library analysis confirms compound ID based on chromatographic retention. This positive spectral conformation significantly enhances confidence in qualitative analytical results.
New levels of data security and traceability The Agilent 1200 Series DAD and MWD SL feature new state-of-the-art electronics and a firmware platform that provide a new level of data security and traceability: • Embedded data recovery card offers a unique “data-never-lost insurance” in the event of communication breakdowns between instrument and PC. • Patented RFID tracking technology for flow cells and UV lamp provides a new level of data traceability by saving all relevant meta data, such as product number, serial number and cell dimensions with each individual raw data file.
Data recovery card offers “data-never-lost insurance”
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www.agilent.com/chem/1200 © Copyright 2008 Agilent Technologies Published January 1, 2008 Publication Number 5989-5574EN
Six detectors to meet all your requirements The Agilent 1200 Series LC offers the UV-visible detector that best suits your needs, from programmable single wavelength to high speed multiwavelength and full spectral detection. • 1200 Series variable wavelength detector (VWD) Lowers detection limits with a simplified design, optimized for high sensitivity and low baseline drift.
• 1200 Series multiple wavelength detector (MWD) Allows simultaneous detection of up to eight different wavelengths without sacrificing sensitivity.
VWD SL
MWD
MWD SL
DAD
DAD SL 1200 DAD SL
1200 DAD For high
1200 MWD SL sensitivity in 1200 MWD
For high sensitivity in ultra-fast, multi-λ analysis
multi-λ and spectral analysis
For high sensitivity in ultra-fast, multi-λ and spectral analysis
• 8 signals • 8 signals • 80-Hz data rate • 20-Hz data rate • Full spectra, For maximum multi-λ analysis • 8 signals 1200 VWD • Full spectra, 80 Hz sensitivity in • 8 signals • 80-Hz data rate 20 Hz • DRC and RFID* For highly ultra-fast • 20-Hz data rate • DRC and RFID* • RFID* sensitive single-λ analysis • RFID* single-λ analysis • 1 signal • 1 signal • 55-Hz data rate • 13-Hz data rate For high
1200 VWD SL sensitivity in
Ne w
The Agilent 1200 Series multiple wavelength and diode array detectors with new low noise flow cells and electronics provide maximum sensitivity and baseline stability. Patented radio frequency identification (RFID) tracking technology for flow cells and UV lamp offers a new level of data traceability.
VWD
Ne w
• 1200 Series variable wavelength detector SL (VWD SL) Enables high-speed UV detection with up to 55-Hz sampling rate for high performance in ultra-fast LC applications.
Performance & Functionality *DRC – Data Recovery Card, RFID – Radio Frequency Identification tags for lamp and flow cell
• 1200 Series multi-wavelength detector SL (MWD SL) Permits detection of up to 8 signals with up to 80-Hz sampling rate, providing uncompromised compatibility with the analysis speed delivered by ultra-fast LC. Embedded data recovery gives unique “data-never-lost insurance”.
• 1200 Series diode array detector (DAD) Provides full power of multiple wavelength detection and spectral information with high resolution and sensitivity.
• 1200 Series diode array detector SL (DAD SL) Detects full spectra and 8 signals with up to 80-Hz sampling rate for ultimate confidence in ultra-fast LC. New data recovery card and RFID technology provide new levels of data security and traceability.
Excellent performance and functionality for a wide range of needs Agilent 1200 Series UV-visible detectors are all designed with a broad range of features to achieve high sensitivity and outstanding productivity: • State-of-the-art optics, electronics and flow cell design provide maximum sensitivity and baseline stability, even under demanding gradient conditions. • A wide range of flow cells enables optimized detector performance for all application needs from nanoflow to preparative LC.
• The 1200 Series instant pilot provides software-independent method and sequence setup, run control and signal display for convenient standalone instrument control. • Broad range of software solutions, including the new Agilent ChemStation, and Agilent EZChrom Elite, provide easy and convenient instrument control, data handling and evaluation, reporting and diagnostics. • Monitoring of multiple signals and spectral data enables immediate review of the separation process results. • Built-in GLP/GMP compliance features include automated wavelength verification, tracking of system performance and maintenance. New data recovery card (DRC) and radio frequency identification RFID technology provide a new level of data security and traceability.
• Checks with preset chromatographic control criteria implement automated performance qualification (PQ). A built-in system suitability software alerts the user and, if necessary, stops the system. • Early maintenance feedback (EMF) issues timely maintenance warnings at predefined limits to keep the system operating at maximum productivity and minimum cost. • Front access to lamps and flow cells enables fast and convenient maintenance for maximum uptime.
Selection of up to eight wavelength signals on the DAD and MWD provides for increased sensitivity and selectivity for medium-complex samples. The latest version of Agilent ChemStation facilitates convenient and fast review of results from large numbers of samples.
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Agilent 1200 Series Variable Wavelength Detector High sensitivity and stability The Agilent 1200 Series VWD provides high sensitivity and improves productivity in routine work: • Minimum baseline noise and maximum baseline stability for lowest detection limits • A wide linear range for reliable, simultaneous quantitation of primary compounds, by-products and impurities • Programmable wavelength switching for sensitivity and selectivity optimized for each analyte’s elution • Stop-flow wavelength scanning for quick wavelength optimization • Automatic wavelength verification by built-in holmium oxide filter
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Flow cells: • Standard
Double beam Deuterium lamp 1 13 Hz ± 0.75 x 10-5 AU at 254 nm 3 x 10-4 AU/h at 254 nm > 2 AU upper limit 190 – 600 nm ± 1 nm, self-calibration with deuterium lines, verification with Holmium oxide filter 6.5 nm typical 14-µL volume, 10-mm cell path length 40 bar (588 psi) maximum pressure 5-µL volume, 6-mm cell path length 40 bar (588 psi) maximum pressure 1-µL volume, 5-mm cell path length 40 bar (588 psi) maximum pressure 14-µL volume, 10-mm cell path length 400 bar (5880 psi) maximum pressure Wavelength, polarity, peak width, lamp on/off Stop-flow wavelength scan Recorder/Integrator 100 mV or 1 V, 1 output Cuvette holder
• Semi-micro • Micro • High pressure Time programmable: Spectral tools: Analog output: Optional: *According to ASTM E1657-98
Redesigned optics provide high signal level, low baseline noise, low drift and minimum refractive index effects.
The Holmium oxide filter swings into the light path to verify that the wavelength matches the setpoints.
3
Agilent 1200 Series Variable Wavelength Detector SL Maximum sensitivity in ultra-fast LC The Agilent 1200 Series VWD SL provides highly sensitive single wavelength analysis in fast and ultra-fast LC applications with peak widths narrower than 1 second. This detector offers: • Sampling rate up to 55 Hz • Minimum baseline noise and maximum baseline stability for lowest detection limits • A wide linear range for reliable, simultaneous quantitation of primary compounds, by-products, and impurities • Programmable wavelength switching for sensitivity and selectivity optimized for each analyte’s elution • Stop-flow wavelength scanning for quick wavelength optimization • Automatic wavelength verification by built-in holmium oxide filter
Specifications Detector type: Light source: Number of signals: Maximum sampling rate Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Flow cells: • Standard
Double beam Deuterium lamp 1 55 Hz ± 0.75 x 10-5 AU at 254 nm 3 x 10-4 AU/h at 254 nm > 2 AU upper limit 190 – 600 nm ± 1 nm, self-calibration with deuterium lines, verification with Holmium oxide filter 6.5 nm typical 14-µL volume, 10-mm cell path length 40 bar (588 psi) maximum pressure 5-µL volume, 6-mm cell path length 40 bar (588 psi) maximum pressure 1-µL volume, 5-mm cell path length 40 bar (588 psi) maximum pressure 14-µL volume, 10-mm cell path length 400 bar (5880 psi) maximum pressure Wavelength, polarity, peak width, lamp on/off Stop-flow wavelength scan Recorder/Integrator 100 mV or 1 V, 1 output Cuvette holder
• Semi-micro • Micro • High pressure Time programmable : Spectral tools: Analog output: Optional: *According to ASTM E1657-98
Using ultra-fast LC conditions with increased data rate significantly improves resolution.
4
Agilent 1200 Series Multiple Wavelength Detector More information and flexibility without loss of sensitivity
The Agilent 1200 Series MWD is a dedicated multiple wavelength detector that offers the same high sensitivity for both single and multiple wavelength detection based on a diode array design with a wavelength range from 190 to 950 nm. • Simultaenous acquisition of up to 8 compound-specific wavelenghts for increased sensitivity and selectivity. • Electronic Temperature Control (ETC) provides maximum baseline stability and practical sensitivity under conditions of fluctuating ambient temperature and humidity. • Wide linear range for reliable quantitation of by-products and impurities, down to levels of < 0.05 % of the primary compound. • Low noise front-end electronics and new, patented flow cell design delivers lowest detection limits resulting from minimized short-term noise (typical < ± 6 µAU ASTM). • Programmable slit for rapid optimization of sensitivity, linearity and spectral resolution. • Range of 9 flow cells provide maximum application flexibility.
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Diode width: Wavelength bunching: Flow cells: • Standard • Semi-micro • Micro • Semi-nano • Nano • High pressure • Preparative • Preparative • Preparative Time programmable: Analog output:
1024-element diode array Deuterium and tungsten lamp 8 20 Hz ± 0.8 x 10-5 AU at 254 nm and at 750 nm < 0.9 x 10-3 AU/h at 254 nm > 2 AU upper limit 190 – 950 nm ± 1 nm, self-calibration with deuterium, lines verification with Holmium oxide filter Programmable: 1, 2, 4, 8, 16 nm < 1 nm Programmable: 2 to 400 nm, in steps of 1 nm 13-µL volume, 10-mm path length, 120 bar (1760 psi) 5-µL volume, 6-mm path length, 120 bar (1760 psi) 2-µL volume, 3-mm path length, 120 bar (1760 psi) 500-nL volume, 10-mm path length, 50 bar (733 psi) 80-nL volume, 6-mm path length, 50 bar 1.7-µL volume, 6-mm path length, 400 bar 3-mm path length, 120 bar (1760 psi) 0.3-mm path length, 20 bar (294 psi) 0.06-mm path length, 20 bar (294 psi) Wavelength, polarity, peak width, on/off lamp on/off bandwidth, autobalance Recorder/Integrator 100 mV or 1 V, 2 outputs
*According to ASTM E1657-98
• Prepared for the future by upgradeability to full spectral capabilities and/or 80 Hz sampling rate. • RFID tags for all flow cells and UV lamp provide new levels of data traceability by
recording parameters, such as product and serial number, production date, cell dimensions, lamp usage, and the last date of a successful cell or lamp test.
mAU 3000
Ginko Biloba extract Active compounds Quercetin
2500
Kaempferol
2000
204 nm
Isorhamnetin
1500 1000
370 nm 500 254 nm 0 2
4
6
8
10
12
14
16
18 Time [min]
Sensitive quantitation of active compounds and impurities in a single run without wavelength switching.
A long-life (> 2000 hours) deuterium lamp reduces operating costs.
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Agilent 1200 Series Multiple Wavelength Detector SL High sensitivity and more information for ultra-fast LC Ultra-fast separations can generate sharp and narrow peaks with peak widths smaller than 1 second. The Agilent 1200 Series multiple wavelength detector SL (MWD SL) offers multiple wavelength detection of 8 signals at up to 80 Hz sampling rate, keeping up with the analysis speed of ultra-fast LC. Even when operated at high data rates, the MWD SL allows precise quantification at trace levels. This exceptional capability assures that your ultra-fast LC methods comply with the strictest regulatory performance requirements. • Diode array design enables simultaneous acquisition of up to 8 compound-specific wavelengths for increased sensitivity and selectivity. • Programmable slit for rapid optimization of sensitivity, linearity and spectral resolution. • Low noise front-end electronics and new, patented flow cell design delivers lowest detection limits resulting from minimized short-term noise (typical < ± 6 µAU ASTM). • Wide linear range for reliable quantification of impurities, down to levels of ,0.05 % for the primary compound. • Electronic Temperature Control (ETC) provides maximum baseline stability and practical sensitivity under conditions of fluctuating ambient temperature and humidity.
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Diode width: Wavelength bunching: Flow cells: • Standard • Semi-micro • Micro • Semi-nano • Nano • High pressure • Preparative • Preparative • Preparative Time programmable: Analog output:
1024-element diode array Deuterium and tungsten lamp 8 80 Hz < ± 0.8 x 10-5 AU at 254 nm and at 750 nm < 0.9 x 10-3 AU/h at 254 nm > 2 AU upper limit 190 – 950 nm ± 1 nm, self-calibration with deuterium, lines verification with Holmium oxide filter Programmable: 1, 2, 4, 8, 16 nm < 1 nm Programmable: 2 to 400 nm, in steps of 1 nm 13-µL, 10-mm path length, 120 bar (1760 psi) 5-µL, 6-mm path length, 120 bar (1760 psi) 2-µL, 3-mm path length, 120 bar (1760 psi) 500-nL, 10-mm path length, 50 bar (733 psi) 80-nL, 6-mm path length, 50 bar 1.7-µL, 6-mm path length, 400 bar 3-mm path length, 120 bar (1760 psi) 0.3-mm path length, 20 bar (294 psi) 0.06-mm path length, 20 bar (294 psi) Wavelength, polarity, peak width, on/off lamp, on/off bandwidth, autobalance Recorder/Integrator 100 mV or 1 V, 2 outputs
*According to ASTM E1657-98
• “Data-never-lost insurance” with built-in Data Recovery Card (DRC) prevents data losses in the event of communication breakdowns between instrument and PC. Raw data and meta data are automatically buffered on an embedded memory card. • RFID tags for all flow cells and UV lamp provide new levels of data traceability by recording parameters,
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such as product and serial number, production date, cell dimensions, lamp usage, and the last date of a successful cell or lamp test. This information is saved with each individual raw data file for unambiguous data traceability, minimizing the risk of false data interpretation. • Range of 9 analytical and preparative flow cells provide maximum application flexibility,
Short-term ASTM noise of the Agilent 1200 Series MWD/ DAD under different conditions Typical noise is well below the specified 16 µAU peak-to-peak under ASTM conditions (4-nm slit, time constant 1 s time, 2.5-Hz data rate). Lowest noise, 4 µAU, is obtained with 2.5 Hz and the 16-nm slit, however, even at 80 Hz and 4-nm slit the noise evel is still sufficient to quantify impurities at 0.05 % level with a signal/noise > 20. This complies with the strictest regulatory requirements. Under ultra-fast LC gradient conditions, baseline noise may increase somewhat. In such cases, a 40-Hz data rate and/or 8 or 16-nm slit can be used to reduce noise and increase sensitivity.
Limit of detection for anthraceneunder ultra-fast LC conditions as low as 300 fg – 10 x faster than in conventional LC The new Agilent 1200 Series MWD SL and DAD SL provide the same or lower limits of detection under ultra-fast conditions as the Agilent 1100 Series MWD and DAD under conventional LC conditions. For a data rate of 10 Hz, the LOD is up to 50 % lower compared to 1100 Series DAD and MWD measured at 2.5 Hz.
LOD of Anthracene under ultra-fast conditions
Data Rate: 80 Hz, LOD = 2.4 pg
Data Rate: 10 Hz, LOD = 0.6 pg
Compare with conventional HPLC LOD/pg on 1100 Binary/DAD “B“
Retention time = 2 min, Peak width = 6 s
LOD/pg in conventional LC on 1100 DAD/MWD “B” Conditions: Conventional LC using 1100 DAD/MWD “B”
Baseline wander of the 1200 MWD/DAD under harsh environmental conditions The new electronic temperature control (ETC) of the 1200 Series MWD/DAD reduces baseline wander by more than a factor of 20. The example demonstrates that, even under the harshest ambient conditions, the baseline of the Agilent 1200 Series DAD remains constant within 30 µAU/°C.
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Agilent 1200 Series Diode Array Detector Complete confidence in spectral data The Agilent 1200 Series diode array detector allows both quantification and confirmation of the analyte identity by spectral libraries at lowest trace level amounts in a single analysis. Verify separation quality with peak purity analysis interactively or automatically in the same run as your analytical quantification. • Deuterium and tungsten lamps with a wavelength range of 190 to 950 nm offer an expanded detection range with maximum light intensity for lowest minimum detection limits. • 1024 diodes and a 1-nm slit achieve high spectral resolution. • Programmable slit (1 to 16 nm) for rapid optimization of sensitivity, linearity and spectral resolution. • Low noise front-end electronics and patented flow cell design delivers lowest detection limits resulting from minimized short-term noise (typical < ± 6 µAU ASTM). • Diode array design enables simultaneous acquisition of up to 8 compound-specific wavelengths for increased sensitivity and selectivity. • Electronic temperature control (ETC) provides maximum baseline stability and practical sensitivity under fluctuating ambient temperature and humidity conditions.
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Diode width: Wavelength bunching: Flow cells: • Standard • Semi-micro • Micro • Semi-nano • Nano • High pressure • Preparative • Preparative • Preparative Time programmable:
• Prepared for the future by upgradeability to 80 Hz sampling rate for ultra-fast separations.
13-µL volume, 10-mm path length, 120 bar (1760 psi) 5-µL volume, 6-mm path length, 120 bar (1760 psi) 2-µL volume, 3-mm path length, 120 bar (1760 psi) 500-nL volume, 10-mm path length, 50 bar (733 psi) 80-nL volume, 6-mm path length, 50 bar 1.7-µL volume, 6-mm path length, 400 bar 3-mm path length, 120 bar (1760 psi) 0.3-mm path length, 20 bar (294 psi) 0.06-mm path length, 20 bar (294 psi) Wavelength, polarity, peak width, on/off lamp, on/off bandwidth, autobalance Data analysis software for spectra evaluation, including spectral libraries and peak purity functions Recorder/Integrator 100 mV or 1 V, 2 outputs
Spectral tools: Analog output: *According to ASTM E1657-98
Absorbance [normalized]
1 nm slit 0.6 0.5 0.4 0.3 0.2 0.1 0
Benzo(g,h,i)perylene Standard, 725 mAUFS
0.7 mAU
DAD 1, 21.801 Sample, 1.1 mAUFS Match factor: 988 230 240 250 260 270 280 nm
• RFID tags for all flow cells and UV lamp provide new levels of data traceability by recording parameters, such as product and serial number, production date, cell dimensions, lamp usage, and the last date of a successful cell or lamp test. • Range of 9 analytical and preparative flow cells provide maximum application flexibility,
1024-element diode array Deuterium and tungsten lamp 8 20 Hz (both spectra and signals) ± 0.8 x 10-5 AU at 254 nm and at 750 nm 0.9 x 10-3 AU/h at 254 nm > 2 AU upper limit 190 – 950 nm ± 1 nm, self-calibration with deuterium, lines verification with Holmium oxide filter Programmable: 1, 2, 4, 8, 16 nm < 1 nm Programmable: 2 to 400 nm, in steps of 1 nm
1 nm
2 nm
4 nm
240 260 8 nm
280 300 320
340
360 380
Wavelength [nm]
Quantitative and qualitative information in trace analysis. Flexible slit setting from fine bands to highest sensitivity. At a setting of 1 nm, and even 0.7 mAUFS, the fine structure of benzene can be revealed. A broad 8-nm or 16-nm slit minimizes baseline noise for enhanced sensitivity.
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Agilent 1200 Series Diode Array Detector SL High sensitivity and confidence in ultra-fast LC Ultra fast separations can generate sharp and narrow peaks with peak widths smaller than 1 second. The Agilent 1200 Series DAD SL offers multiple wavelength and full spectral detection at up to 80 Hz sampling rate, keeping up with the analysis speed of ultra-fast LC. Even when operated at high data rates, the 1200 Series DAD SL allows precise identification, quantification and peak purity analysis at trace levels. This exceptional capability assures that your ultra-fast LC methods comply with the strictest regulatory performance requirements.
Specifications Detector type: Light source: Number of signals: Maximum sampling rate: Short-term noise:* Drift:* Linearity: Wavelength range: Wavelength accuracy: Slit width: Diode width: Wavelength bunching: Flow cells: • Standard • Semi-micro • Micro • Semi-nano • Nano • High pressure • Preparative • Preparative • Preparative Time programmable: Spectral tools: Analog output:
1024-element diode array Deuterium and tungsten lamp 8 80 Hz (both spectra and signals) < ± 0.8 x 10-5 AU at 254 nm and at 750 nm < 0.9 x 10-3 AU/h at 254 nm > 2 AU upper limit 190 – 950 nm ± 1 nm, self-calibration with deuterium, lines verification with Holmium oxide filter Programmable: 1, 2, 4, 8, 16 nm < 1 nm Programmable: 2 to 400 nm, in steps of 1 nm 13-µL, 10-mm path length, 120 bar (1760 psi) 5-µL, 6-mm path length, 120 bar (1760 psi) 2-µL, 3-mm path length, 120 bar (1760 psi) 500-nL, 10-mm path length, 50 bar (733 psi) 80-nL, 6-mm path length, 50 bar 1.7-µL, 6-mm path length, 400 bar 3-mm path length, 120 bar (1760 psi) 0.3-mm path length, 20 bar (294 psi) 0.06-mm path length, 20 bar (294 psi) Wavelength, polarity, peak width, on/off lamp, on/off bandwidth, autobalance Data analysis software for spectra evaluation, including spectral libraries and peak purity functions Recorder/Integrator 100 mV or 1 V, 2 outputs
*According to ASTM E1657-98
80-Hz full spectral sampling rate provides resolution and peak capacity gains of 90 % (30 %) relative to 10 Hz (20 Hz) sampling rates.
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• Full spectral data acquisition rate of 80 Hz increases resolution by up to 100 % and delivers ultra-fast, highly sensitive peak purity analysis and spectral conformation, even at trace levels. • Dual lamp design for highest sensitivity from 190 nm to 950 nm. • Diode array design enables simultaneous acquisition of up to 8 compound-specific wavelengths for increased sensitivity and selectivity. • Programmable slit (1 to 16 nm) for rapid optimization of sensitivity, linearity and spectral resolution provides optimum incident light conditions.
• Low noise front-end electronics and patented flow cell design delivers lowest detection limits resulting from minimized short-term noise (typical < ± 6 µAU ASTM). • Electronic temperature control (ETC) provides maximum baseline stability and practical sensitivity under fluctuating ambient temperature and humidity conditions. • “Data-never-lost insurance” with built-in data recovery card (DRC) prevents data losses in the event of communication breakdowns between instrument and PC by automatically buffering raw data and meta data on an embedded memory card.
• RFID tags for all flow cells and UV lamp provide new levels of data traceability by recording parameters, such as product and serial number, production date, cell dimensions, lamp usage, and the date of the last successful cell or lamp test. This information is saved with each individual raw data file for unambiguous data traceability, minimizing the risk of false data interpretation. • Range of 9 analytical and preparative flow cells provide maximum flexibility, compatibility and investment protection.
• Reference wavelength eliminates background interference.
Under ultra-fast conditions, the DAD/MWD SL allows simultaneous identification and quantification of primary compounds and impurities at 0.03 % level – in a single run with the same or better precision and signal/noise provided by conventional LC.
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Peak purity analysis of trace level compounds under ultra-fast LC conditions The 1200 DAD SL enables peak purity analysis under ultra-fast LC conditions, even for trace level compounds at < 0.1 % level. In this case, the spectral analysis confirms that the nifedipin peak as identified by chromatographic retention was pure. This positive purity confirmation significantly increases confidence in quantitative chromatographic results. The exceptional capability of accurate spectral analysis at trace levels is enabled by the dual lamp design of the Agilent 1200 Series DAD SL. It provides lowest noise over the complete wavelength range for significantly increased spectral quality.
Spectral conformation of trace level compounds at < 0.1 % level under ultra-fast LC conditions A library search is executed for the measured trace level spectrum. Match factors are calculated and tabulated in the Library Search Result table. Highest spectral match is achieved for nifedipin with a match factor of 962 compared to 929 for the primary compound nimodipin, which shows high spectral similarity to nifedipin. The spectral library analysis confirms compound ID based on chromatographic retention. This positive spectral conformation significantly enhances confidence in qualitative analytical results.
New levels of data security and traceability The Agilent 1200 Series DAD and MWD SL feature new state-of-the-art electronics and a firmware platform that provide a new level of data security and traceability: • Embedded data recovery card offers a unique “data-never-lost insurance” in the event of communication breakdowns between instrument and PC. • Patented RFID tracking technology for flow cells and UV lamp provides a new level of data traceability by saving all relevant meta data, such as product number, serial number and cell dimensions with each individual raw data file.
Data recovery card offers “data-never-lost insurance”
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www.agilent.com/chem/1200 © Copyright 2008 Agilent Technologies Published January 1, 2008 Publication Number 5989-5574EN
