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Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f BMC Complementary and Alternative Medicine 2012, 12:30
doi:10.1186/1472-6882-12-30
Dinesh SINGH Moirangthem (
[email protected]) Narayan CHANDRA Talukdar (
[email protected]) Naresh Kasoju (
[email protected]) Utpal Bora (
[email protected])
ISSN Article type
1472-6882 Research article
Submission date
2 December 2011
Acceptance date
3 April 2012
Publication date
3 April 2012
Article URL
http://www.biomedcentral.com/1472-6882/12/30
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Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f Dinesh Singh Moirangthem1 Email:
[email protected] Narayan Chandra Talukdar1* * Corresponding address Email:
[email protected] or
[email protected] Naresh Kasoju2 Email:
[email protected] Utpal Bora2 Email:
[email protected] 1
Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat Institutional Area, Imphal 795001, Manipur, India 2
Indian Institute of Technology Guwahati, Department of Biotechnology, Guwahati 781039, Assam, India
Abstract Background Cephalotaxus spp. are known to possess various therapeutic potentials. Cephalotaxus griffithii, however, has not been evaluated for its biological potential. The reason may be the remoteness and inaccessibility of the habitat where it is distributed. The main aim of this study was to: (1) evaluate multiple biological potentials of stem bark of C. griffithii, and (2) identify solvent extract of stem bark of C. griffithii to find the one with the highest specific biological activity.
Methods Dried powder of stem bark of C. griffithii was exhaustively extracted serially by soaking in petroleum ether, acetone and methanol to fractionate the chemical constituents into individual fractions or extracts. The extracts were tested for total phenolic and flavonoid content, antioxidant (DPPH radical scavenging, superoxide radical scavenging, and reducing power models), antibacterial (disc diffusion assay on six bacterial strains), cytotoxic (MTT assay on HeLa cells), and apoptotic activity (fluorescence microscopy, DNA fragmentation assay, and flow cytometry on HeLa cells).
Results Among the three extracts of stem bark of C. griffithii, the acetone extract contained the highest amount of total phenolics and flavonoids and showed maximum antioxidant, antibacterial, cytotoxic (IC50 of 35.5 ± 0.6 μg/ml; P