Drinking Water Scheme - Gov.uk

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Summary of Results External Quality Assessment of Water Microbiology

Drinking Water Scheme Distribution Number: W178

Sample Numbers: W178A, W178B, W178C

Distribution Date: Results Due: Report Date:

November 2017 08 December 2017 18 December 2017

Samples prepared and quality control tested by:

Angela Appea Richard Borrill Thomas Harper Zak Prior Judith Spellar Aneta Stranc Lili Tsegaye

Data analysed by:

Nita Patel Manchari Rajkumar

Report compiled by:

Nita Patel Manchari Rajkumar

Authorised by:

Nita Patel

This report must not be reproduced without permission of the organisers. Public Health England Food and Environmental Proficiency Testing Unit (FEPTU) 61 Colindale Avenue London NW9 5EQ Tel: +44 (0)20 8327 7119 Fax: +44 (0)20 8200 8264 Email: [email protected] The data in FEPTU reports is confidential

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For further information on the scheme please refer to: Scheme Guide: https://www.gov.uk/government/publications/food-and-water-proficiency-testing-schemes-scheme-guide Guide to Scoring and Statistics: https://www.gov.uk/government/publications/food-and-water-proficiency-testing-schemes-scoring-systems-and-statistics

General guidance for z-scores: Participants' enumeration results are converted into z-scores using the following formula:

Z = (xi-Xpt) σpt

xi Xpt

= participants' result expressed (expressed as a log 10 value) = assigned value (participants' consensus median (expressed as a log 10 value))

σpt

= the fixed standard deviation for the examination (calculated by FEPTU)

The σpt-value expresses the acceptable difference between the individual participant 's result and the participants' consensus median. The σpt-value used for calculating z-scores for all parameters in the Drinking Water Scheme is 0.35. A guide to interpreting z-scores follows, although laboratories must interpret their scores in the context of their own laboratory situation. z = -1.99 to +1.99 z = -2 to -2.99 or +2 to +2.99 z= < -3.00 or > + 3.00

satisfactory questionable unsatisfactory

It is usually recommended that z-scores exceeding +/-2 are investigated to establish the possible cause. rule, PHE recommends that all questionable and unsatisfactory results are investigated.

As a general

FEPTU Quality Control: To demonstrate homogeneity of the sample, a minimum of 10 LENTICULE® discs, selected randomly from a batch, are tested in duplicate for parameters requiring enumeration.

To demonstrate stability of the sample, a minimum of nine LENTICULE discs, selected randomly from a batch, are examined throughout the distribution period for enumeration parameters. PHE uses methods stipulated in the DWI 's series of documents: The Microbiology of Drinking Water (2002) - Methods for the Examination of Waters and Associated Materials. The FEPTU results are used for guidance in the preliminary intended results notification, letters are posted on the website immediately after every distribution; electronic notification of their availability is sent to all participants. Refer to section 17.0 of the Scheme Guide if you have experienced difficulties with any of the examinations . https://www.gov.uk/government/publications/food-and-water-proficiency-testing-schemes-scheme-guide

Participants are reminded that reporting an incorrect or false negative results could have serious public health implications. Participants who report unexpectedly high or low results for enumerations on single occasions only should not be unduly alarmed, although they should still assess the reason(s) for the outlying result. The results, as summarised in the performance assessment sheet included in this report, provide a more effective indication of on-going problems with the enumeration of low levels of indicator organisms in drinking water samples. Performance assessment cannot be undertaken as effectively if laboratories do not participate in all distributions for the Drinking Water Scheme.

Please contact FEPTU staff for advice and information: Repeat samples

Carmen Gomes or Kermin Daruwalla

Data Analysis

Manchari Rajkumar or Nita Patel

Microbiological advice

Nita Patel or Zak Prior

General comments and complaints

Nita Patel or Zak Prior

Scheme consultants

Julie E. Russell

Scheme Co-ordinator

Nita Patel

Tel: Fax: Email:

+44 (0)20 8327 7119 +44 (0)20 8200 8264 [email protected]

FEPTU's website

Accreditation: PHE Water EQA Scheme for Drinking Water is accredited by the United Kingdom Accreditation Service (UKAS) to ISO/IEC 17043:2010.

0006 The data in FEPTU reports is confidential

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Sample: W178A Contents:

Escherichia coli (wild strain), Enterococcus faecium (NCIMB 12672), Clostridium perfringens (wild strain), Kocuria kristinae (NCTC 11038), Pseudomonas fluorescens (wild strain)

Expected Results: All counts are expressed as colony forming units (cfu) per 100mL except the colony counts at 37ºC/48h and 22ºC/72h which are cfu per mL.

Colony count 22ºC/72h

Colony count 37ºC/48h

C.perfringens

P.aeruginosa

Enterococci

E.coli

Parameter

Coliform bacteria

The fixed standard deviation value (σpt value) used for calculation of the z-scores is 0.35 for all parameters.

FEPTU median

17

17

146

0

36

49

48

No. results returned

134

140

126

114

106

127

123

Assigned value

31

30

130

0

42

45

44

Uncertainty of assigned value*

0.03

0.02

0.01

N/A

0.02

0.01

0.01

Participants mean (all results)

29

28

128

0

41

45

44

7 - 98

6 - 95

41 - 411

N/A

13 - 133

14 - 142

14 - 139

0.25

0.23

0.08

N/A

0.16

0.08

0.1

No of outlying counts

20

16

6

N/A

8

9

14

False positives

N/A

N/A

N/A

1

N/A

N/A

N/A

False negatives

5

7

0

N/A

3

1

1

(Participants median all results)

Expected Range

Standard deviation** ( log₁₀)

Your result

Score for performance assessment

Z-score

Analyst 2 Result

Analyst 2 Z-score

Analyst 3 Result

Analyst 3 Z-score

*

U(Xpt) is based on results transformed to a log₁₀ scale

**

Robust S* based on median absolute deviation about the participants' median (MADe) and is based on logged data

Total sent samples

152

Not examined

8

Non returns

3

Late returns

0

The data in FEPTU reports is confidential

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Sample: W178B Contents:

Escherichia coli (wild strain), Enterobacter cloacae (wild strain), Pseudomonas aeruginosa (wild strain), Clostridium paraputrificum (wild strain), Microbacterium sp. (wild strain)

Expected Results: All counts are expressed as colony forming units (cfu) per 100mL except the colony counts at 37ºC/48h and 22ºC/72h which are cfu per mL.

0

64

0

2

72

No. results returned

135

140

125

114

106

126

122

Assigned value

106

74

0

38

0

2

76

Uncertainty of assigned value*

0.04

0.04

N/A

0.03

N/A

0.05

0.01

Participants mean (all results)

109

73

0

35

0

3

75

34 - 335

13 - 234

N/A

12 - 119

N/A

0-9

24 - 240

0.3

0.36

N/A

0.23

N/A

0.37

0.1

No of outlying counts

11

18

N/A

12

N/A

12

13

False positives

N/A

N/A

9

N/A

2

N/A

N/A

False negatives

3

5

N/A

2

N/A

0

2

E.coli

Parameter

Colony count 22ºC/72h

Colony count 37ºC/48h

39

P.aeruginosa

65

Enterococci

FEPTU median

Coliform bacteria

C.perfringens

The fixed standard deviation value (σpt value) used for calculation of the z-scores is 0.35 for all parameters.

(Participants median all results)

Expected Range

Standard deviation** ( log₁₀)

Your result

Score for performance assessment

Z-score

Analyst 2 Result

Analyst 2 Z-score

Analyst 3 Result

Analyst 3 Z-score

*

U(Xpt) is based on results transformed to a log₁₀ scale

**

Robust S* based on median absolute deviation about the participants' median (MADe) and is based on logged data

Total sent samples

152

Not examined

7

Non returns

3

Late returns

0

The data in FEPTU reports is confidential

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Sample: W178C Contents:

Enterococcus faecalis (wild strain), Pseudomonas aeruginosa (wild strain), Clostridium perfringens (wild strain), Lactococcus lactis (wild strain), Providencia rettgeri (NCTC 7475), Staphylococcus epidermidis (wild strain)

Expected Results: All counts are expressed as colony forming units (cfu) per 100mL except the colony counts at 37ºC/48h and 22ºC/72h which are cfu per mL.

Colony count 22ºC/72h

41

48

16

13

134

140

126

114

106

127

122

0

0

44

28

58

14

11

Uncertainty of assigned value*

N/A

N/A

0.01

0.02

0.01

0.03

0.02

Participants mean (all results)

0

0

44

27

57

13

12

Expected Range

N/A

N/A

14 - 139

9 - 89

18 - 183

4 - 44

3 - 35

Standard deviation**

N/A

N/A

0.1

0.18

0.13

0.24

0.16

N/A

N/A

3

13

7

12

12

False positives

6

1

N/A

N/A

N/A

N/A

N/A

False negatives

N/A

N/A

0

3

2

0

4

No. results returned

Assigned value

C.perfringens

44

P.aeruginosa

0

FEPTU median

Enterococci

E.coli

0

Parameter

Coliform bacteria

Colony count 37ºC/48h

The fixed standard deviation value (σpt value) used for calculation of the z-scores is 0.35 for all parameters.

(Participants median all results)

( log₁₀) No of outlying counts

Your result

Score for performance assessment

Z-score

Analyst 2 Result

Analyst 2 Z-score

Analyst 3 Result

Analyst 3 Z-score

*

U(Xpt) is based on results transformed to a log₁₀ scale

**

Robust S* based on median absolute deviation about the participants' median (MADe) and is based on logged data

Total sent samples

152

Not examined

7

Non returns

3

Late returns

0

The data in FEPTU reports is confidential

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Printed at 14:04 on Monday, 18 December, 2017

1

Outlying results

Outlying results

5-6

PHE score 30

25

0

2

Outlying results

0

98

88-98

83-87

78-82

73-77

68-72

63-67

58-62

53-57

48-52

43-47

38-42

33-37

28-32

23-27

18-22

7-12

13-17

0

Count per 100mL

Method based presentation W178A : Coliform bacteria

FEPTU Method: Chromogenic coliform agar

Method

Robust S*

Number of Results

Excluded Results

Percentage of the total

Median

Range Reported

Colilert (18/250)

39

0

29

36

0.17

0

-

165

Colisure

0

0

0

Tube combinations

3

1

2

Other (MPN)

3

0

2

MLSB

18

0

13

24

0.22

4

-

68

MLSA

4

0

3 20

0.29

0

-

50

(Log₁₀)

MLGA

13

0

9

Tergitol 7 (TTC)

3

0

2

Chromogenic coliform agar

39

0

29

32

0.29

0

-

190

Other (Membrane filtration)

11

0

8

23

0.39

1

-

44

31 Participant Median 17 FEPTU Median

Outlying results

Outlying results

5-6

20

15

10

5

>98

88-98

83-87

78-82

73-77

68-72

63-67

58-62

53-57

48-52

43-47

38-42

33-37

28-32

23-27

18-22

13-17

0

7-12

No. of Results

25

Outlying results

30

95

86-95

81-85

76-80

71-75

66-70

61-65

56-60

51-55

46-50

41-45

36-40

31-35

26-30

21-25

16-20

12-15

0

6-11

No. of Results

45

Outlying results

50

1-5

55

Outlying results

60

411

372-411

352-371

332-351

312-331

292-311

272-291

252-271

232-251

213-231

194-212

175-193

156-174

137-155

118-136

99-117

80-98

61-79

411

372-411

352-371

332-351

312-331

292-311

272-291

Count per 100mL

252-271

232-251

213-231

194-212

175-193

156-174

137-155

118-136

99-117

80-98

61-79

41-60

0

133

120-133

113-119

106-112

99-105

92-98

85-91

79-84

73-78

67-72

61-66

55-60

49-54

43-48

37-42

31-36

25-30

19-24

133

120-133

113-119

99-105

92-98

106-112

Count per 100mL TSC (Membrane Filtration)

85-91

79-84

73-78

67-72

61-66

55-60

49-54

43-48

37-42

31-36

25-30

19-24

13-18

0

142

129-142

122-128

115-121

108-114

101-107

94-100

87-93

80-86

73-79

66-72

59-65

52-58

45-51

39-44

33-38

27-32

21-26

14-20

142

129-142

122-128

115-121

108-114

94-100

101-107

Count per mL

87-93

80-86

73-79

66-72

59-65

52-58

45-51

39-44

33-38

27-32

21-26

14-20

0

335

302-335

285-301

268-284

251-267

234-250

217-233

Count per 100mL

200-216

183-199

166-182

149-165

132-148

115-131

99-114

83-98

67-82

51-66

0

34-50

No. of Results

25

Outlying results

30

234

209-234

196-208

183-195

171-182

159-170

147-158

Count per 100mL

135-146

123-134

111-122

99-110

87-98

75-86

63-74

51-62

39-50

27-38

0

13-26

No. of Results

25

Outlying results

30

119

108-119

96-101

90-95

84-89

78-83

102-107

Count per 100mL Pseudalert (MPN)

72-77

66-71

60-65

54-59

48-53

42-47

36-41

30-35

24-29

18-23

0

12-17

No. of Results

25

Outlying results

30

139

126-139

119-125

112-118

105-111

98-104

91-97

84-90

77-83

70-76

63-69

56-62

50-55

44-49

38-43

32-37

26-31

20-25

139

126-139

119-125

112-118

98-104

105-111

Count per 100mL

91-97

84-90

77-83

70-76

63-69

56-62

50-55

44-49

38-43

32-37

26-31

20-25

14-19

0

89

80-89

75-79

70-74

65-69

60-64

55-59

50-54

45-49

41-44

37-40

33-36

29-32

25-28

21-24

17-20

13-16

0

9-12

No. of Results

25

Outlying results

30

183

166-183

157-165

148-156

139-147

130-138

121-129

112-120

103-111

94-102

85-93

76-84

67-75

59-66

51-58

43-50

35-42

27-34

183

166-183

157-165

148-156

139-147

130-138

121-129

Count per 100mL TSC (Membrane Filtration)

112-120

103-111

94-102

85-93

76-84

67-75

59-66

51-58

43-50

35-42

27-34

18-26

0

44

39-44

36-38

33-35

31-32

29-30

27-28

25-26

23-24

21-22

19-20

17-18

15-16

13-14

11-12

9-10

7-8

0

4-6

No. of Results

25

Outlying results

30

41

No. of Results

30

11 Participant Median 13 FEPTU Median

35-41

35

Outlying results

40

25

20

15

10

5

32-35

30-31

28-29

26-27

24-25

22-23

20-21

18-19

16-17

14-15

12-13

10-11

8-9

6-7

5-5

4-4

3-3

41

35-41

32-35

30-31

28-29

26-27

24-25

22-23

20-21

18-19

16-17

14-15

12-13

10-11

8-9

6-7

5-5

4-4

3-3

0