Electrophoresis File Run Summary
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Page Created: Modified:
Electrophoresis File Run Summary
Instrument Information: Instrument Name: Serial#:
Assay Information: Assay Origin Path: Assay Class: Version:
Assay Comments:
DE72901887
1 of 19
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Firmware: C.01.069
DE72901887
Type:
G2939A
C:\Program Files\Agilent\2100 bioanalyzer\2100 expert\assays\dsDNA\High Sensitivity DNA.xsy High Sensitivity DNA Assay 1.0
Copyright © 2003-2009 Agilent Technologies
Chip Information: Chip Lot #:
Reagent Kit Lot #: Chip Comments:
Purified-Fragmented DNA 500ngSt
Purified-Fragmented DNA 2ugSt
25min
20min
15min
10min
0min
Ladder
2100 Expert (B.02.07.SI532)
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electrophoresis File Run Summary (Chip Summary) Sample Name Sample Rest. Sta Observation Comment Digest tus
Result Label
Page
2 of 19
Created: Modified:
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Printed:
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Result Color
Purified-Fragmen ted DNA 500ngSt Purified-Fragmen ted DNA 2ugSt 25min 20min 15min 10min 0min sample sample sample sample
8 9 10 11
Chip Lot #
Reagent Kit Lot #
Chip Comments :
2100 Expert (B.02.07.SI532)
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electrophoresis Assay Details
Page
3 of 19
Created: Modified:
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Printed:
4/28/2010 5:45:27 PM
General Analysis Settings
Number of Available Sample and Ladder Wells (Max.) : 12 Minimum Visible Range [s] : 32 Maximum Visible Range [s] : 138 Start Analysis Time Range [s] : 33 End Analysis Time Range [s] : 137.5 Ladder Concentration [pg/µl] : 1950 Uses Standard Area for Ladder Fragments Lower Marker Concentration [pg/µl] : 125 Upper Marker Concentration [pg/µl] : 75 Used Upper Marker for Quantitation Standard Curve Fit is Point to Point Show Data Aligned to Lower and Upper Marker
Integrator Settings
Integration Start Time [s] : 33.05 Integration End Time [s] : 137 Slope Threshold : 0.8 Height Threshold [FU] : 5 Area Threshold : 0.1 Width Threshold [s] : 0.6 Baseline Plateau [s] : 0.5
Filter Settings
Filter Width [s] : 0.5 Polynomial Order : 4
Ladder
Ladder Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Size
35 50 100 150 200 300 400 500 600 700 1000 2000 3000 7000 10380
2100 Expert (B.02.07.SI532)
Area 160 210 208 221 242 270 305 306 336 321 366 413 411 400 214
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
Page
7 of 19
Created: Modified:
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Printed:
4/28/2010 5:45:27 PM
25min
Overall Results for sample 3 : Number of peaks found:
30
Noise:
0.5
Peak table for sample 3 : Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26
Size [bp] 35 57 66 81 97 108 165 183 212 248 270 281 296 307 353 402 414 455 471 488 558 577 629 651 689 735
2100 Expert (B.02.07.SI532)
25min Corr. Area 1:
25min
Conc. [pg/µl] 125.00 277.12 123.26 101.42 63.57 84.07 431.11 157.75 183.94 376.96 177.73 117.63 88.47 107.80 410.77 312.87 94.77 320.57 133.37 159.30 493.40 146.80 315.82 137.77 212.88 182.03
Molarity [pmol/l] 5,411.2 7,313.3 2,814.5 1,904.8 995.3 1,181.6 3,947.3 1,305.6 1,314.0 2,303.0 998.2 633.8 453.0 532.0 1,762.9 1,178.9 346.7 1,066.7 429.2 494.7 1,339.8 385.5 761.2 320.8 468.5 375.5
5,743.5
Observations Lower Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Created: Modified:
Electropherogram Summary Continued ... ... Peak table for sample 3 : Peak 27 28 29 30 31 32
Size [bp] 838 1,145 1,271 1,334 1,428 10,380
Region table for sample 3 : From [bp] 200
To [bp] Corr. Area 1,000 5,743.5
2100 Expert (B.02.07.SI532)
25min
Conc. [pg/µl] 321.92 681.92 156.61 189.12 1,253.62 75.00
% of Total 60
25min
Average Size [bp] 539
Molarity [pmol/l] 582.2 902.5 186.7 214.8 1,329.7 10.9
Size distribution in CV [%] 39.7
Page
8 of 19
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Observations
Upper Marker Conc. [pg/µl] 5,301.65
Molarity [pmol/l] 19,240.0
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
Page
9 of 19
Created: Modified:
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Printed:
4/28/2010 5:45:27 PM
20min
Overall Results for sample 4 : Number of peaks found:
30
Noise:
0.3
Peak table for sample 4 : Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26
Size [bp] 35 43 57 66 75 80 97 168 188 236 253 262 272 296 308 327 337 351 356 375 397 412 504 544 625 668
2100 Expert (B.02.07.SI532)
20min Corr. Area 1:
20min
Conc. [pg/µl] 125.00 71.49 127.61 72.82 49.89 100.76 52.55 223.09 136.71 275.82 131.83 59.71 74.00 194.06 74.32 145.41 78.46 89.09 76.00 75.68 110.72 100.64 384.18 188.98 384.93 226.31
Molarity [pmol/l] 5,411.3 2,490.1 3,375.3 1,663.3 1,008.8 1,899.8 820.0 2,008.6 1,100.4 1,767.6 788.0 344.9 411.9 993.8 365.2 673.8 352.9 385.0 323.3 306.0 422.4 369.7 1,153.9 526.4 933.6 513.5
5,378.9
Observations Lower Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Created: Modified:
Electropherogram Summary Continued ... ... Peak table for sample 4 : Peak 27 28 29 30 31 32
Size [bp] 1,107 1,417 1,561 1,640 10,380 12,592
Region table for sample 4 : From [bp] 200
To [bp] Corr. Area 1,000 5,378.9
2100 Expert (B.02.07.SI532)
20min
Conc. [pg/µl] 1,226.29 537.24 225.53 1,542.78 75.00 0.00
% of Total 52
20min
Average Size [bp] 569
Molarity [pmol/l] 1,679.1 574.3 218.9 1,425.0 10.9 0.0
Size distribution in CV [%] 38.2
Page 10 of 19 4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Observations
Upper Marker
Conc. [pg/µl] 4,369.05
Molarity [pmol/l] 14,964.2
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
Page 11 of 19 Created: Modified:
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Printed:
4/28/2010 5:45:27 PM
15min
Overall Results for sample 5 : Number of peaks found:
22
Noise:
0.4
Peak table for sample 5 : Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Size [bp] 35 58 67 82 91 99 142 171 197 213 241 255 268 279 294 354 369 395 485 1,655 1,928 10,380 13,489 14,663
2100 Expert (B.02.07.SI532)
15min Corr. Area 1:
15min
Conc. [pg/µl] 125.00 114.02 45.27 96.19 36.73 36.35 58.50 29.95 83.07 56.04 45.34 56.23 57.91 51.32 68.74 271.60 44.00 53.94 337.32 2,219.66 1,565.82 75.00 0.00 0.00
Molarity [pmol/l] 5,411.3 3,003.9 1,022.4 1,784.4 612.9 558.9 622.4 265.1 638.2 398.2 285.1 334.3 327.8 278.4 354.0 1,161.8 180.6 207.0 1,054.5 2,031.6 1,230.2 10.9 0.0 0.0
4,907.2
Observations Lower Marker
Upper Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Created: Modified:
Electropherogram Summary Continued ... ... Region table for sample 5 : From [bp] 200
To [bp] Corr. Area 1,000 4,907.2
2100 Expert (B.02.07.SI532)
% of Total 41
15min
Average Size [bp] 592
Size distribution in CV [%] 36.9
Conc. [pg/µl] 2,942.36
Page 12 of 19
Molarity [pmol/l] 9,653.8
© Copyright 2003 - 2009 Agilent Technologies, Inc.
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
Page 13 of 19 Created: Modified:
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Printed:
4/28/2010 5:45:27 PM
10min
Overall Results for sample 6 : Number of peaks found:
24
Noise:
0.3
Peak table for sample 6 : Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26
Size [bp] 35 57 67 82 108 157 173 196 214 224 233 248 264 273 298 316 365 381 423 492 1,987 2,265 2,619 10,380 16,087 17,911
2100 Expert (B.02.07.SI532)
10min Corr. Area 1:
10min
Conc. [pg/µl] 125.00 4.19 6.81 14.36 19.70 5.94 4.82 4.74 8.66 5.02 4.53 8.77 6.67 9.56 9.94 14.29 53.19 17.46 37.96 81.96 1,280.20 150.97 1,008.52 75.00 0.00 0.00
Molarity [pmol/l] 5,411.2 111.2 153.6 265.9 277.2 57.2 42.3 36.7 61.2 33.9 29.4 53.6 38.2 53.1 50.5 68.6 220.6 69.4 136.1 252.4 976.0 101.0 583.4 10.9 0.0 0.0
3,244.2
Observations Lower Marker
Upper Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Created: Modified:
Electropherogram Summary Continued ... ... Region table for sample 6 : From [bp] 200
To [bp] Corr. Area 1,000 3,244.2
2100 Expert (B.02.07.SI532)
% of Total 27
10min
Average Size [bp] 616
Size distribution in CV [%] 35.8
Conc. [pg/µl] 1,130.80
Page 14 of 19
Molarity [pmol/l] 3,570.1
© Copyright 2003 - 2009 Agilent Technologies, Inc.
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Page 15 of 19 Created: Modified:
Electropherogram Summary Continued ...
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
0min
Overall Results for sample 7 : Number of peaks found:
6
Noise:
0.3
Peak table for sample 7 : Peak 1 2 3 4 5 6 7 8
Conc. [pg/µl]
Region table for sample 7 : From [bp] 200
To [bp] Corr. Area 1,000 0.0
2100 Expert (B.02.07.SI532)
Corr. Area 1:
0min
Size [bp] 35 6,394 9,205 10,380 17,254 21,426 32,412 33,176
0min
125.00 13.74 23.36 75.00 0.00 0.00 0.00 0.00
% of Total 0
0min
Average Size [bp] 0
Molarity [pmol/l] 5,411.2 3.3 3.8 10.9 0.0 0.0 0.0 0.0
Size distribution in CV [%] 0.0
0.0
Observations Lower Marker Upper Marker
Conc. [pg/µl] 0.00
Molarity [pmol/l] 0.0
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
Page 16 of 19 Created: Modified:
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Printed:
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Ladder
Setpoint Deviations for sample 12 : Height Threshold [FU] :
20
Area Threshold :
0
Ladder Baseline Plateau [s] :
0.3
Overall Results for Ladder Noise:
0.3
Peak table for Ladder Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Size [bp] 35 50 100 150 200 300 400 500 600 700 1,000 2,000 3,000 7,000 10,380
2100 Expert (B.02.07.SI532)
Conc. [pg/µl] 125.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 75.00
Molarity [pmol/l] 5,411.3 4,545.5 2,272.7 1,515.2 1,136.4 757.6 568.2 454.5 378.8 324.7 227.3 113.6 75.8 32.5 10.9
Observations Lower Marker Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Upper Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
Gel Image
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
2100 Expert (B.02.07.SI532)
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Page 17 of 19 Created: Modified:
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Printed:
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2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
Curves
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Page 18 of 19 Created: Modified:
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Printed:
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Standard Curve
2100 Expert (B.02.07.SI532)
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
Run Logbook Description
Page 19 of 19
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Category
Run started on port 5 (File: C:\Program Files\Agilent\21 00 bioanalyzer\210 0 expert\Data\201 0-04-28\2100 expert_High Sensitivity DNA Assay_DE72901 887_2010-04-28 _17-13-27.xad)
Instrument
Run
4/28/2010 5:13:33 PM
(GMT +09:00) Administrator Tokyo Standard Time
BIOANALYZER
Product Number : G2939A
Instrument
Run
4/28/2010 5:13:33 PM
Administrator
BIOANALYZER
Name :
Instrument
Run
4/28/2010 5:13:33 PM
Administrator
BIOANALYZER
Vendor : Agilent Technologies
Instrument
Run
4/28/2010 5:13:33 PM
Administrator
BIOANALYZER
Serial# : DE72901887
Instrument
Run
4/28/2010 5:13:33 PM
Administrator
BIOANALYZER
Firmware : C.01.069
Instrument
Run
4/28/2010 5:13:33 PM
Administrator
BIOANALYZER
Cartridge : Electrode
Instrument
Run
4/28/2010 5:13:33 PM
(GMT +09:00) Tokyo Standard Time (GMT +09:00) Tokyo Standard Time (GMT +09:00) Tokyo Standard Time (GMT +09:00) Tokyo Standard Time (GMT +09:00) Tokyo Standard Time (GMT +09:00) Tokyo Standard Time
Administrator
BIOANALYZER
2100 Expert (B.02.07.SI532)
Instrument
Run
Sub Category Time Stamp 4/28/2010 5:43:21 PM
Time Zone
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Source
Run ended on port 5 (Number of wells acquired: 8)
Number
Created: Modified: User
(GMT +09:00) Administrator Tokyo Standard Time
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Printed:
Host
BIOANALYZER
4/28/2010 5:45:27 PM
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Page Created: Modified:
Electrophoresis File Run Summary
Instrument Information: Instrument Name: Serial#:
Assay Information: Assay Origin Path: Assay Class: Version:
Assay Comments:
DE72901887
1 of 19
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Firmware: C.01.069
DE72901887
Type:
G2939A
C:\Program Files\Agilent\2100 bioanalyzer\2100 expert\assays\dsDNA\High Sensitivity DNA.xsy High Sensitivity DNA Assay 1.0
Copyright © 2003-2009 Agilent Technologies
Chip Information: Chip Lot #:
Reagent Kit Lot #: Chip Comments:
Purified-Fragmented DNA 500ngSt
Purified-Fragmented DNA 2ugSt
25min
20min
15min
10min
0min
Ladder
2100 Expert (B.02.07.SI532)
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electrophoresis File Run Summary (Chip Summary) Sample Name Sample Rest. Sta Observation Comment Digest tus
Result Label
Page
2 of 19
Created: Modified:
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Printed:
4/28/2010 5:45:27 PM
Result Color
Purified-Fragmen ted DNA 500ngSt Purified-Fragmen ted DNA 2ugSt 25min 20min 15min 10min 0min sample sample sample sample
8 9 10 11
Chip Lot #
Reagent Kit Lot #
Chip Comments :
2100 Expert (B.02.07.SI532)
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electrophoresis Assay Details
Page
3 of 19
Created: Modified:
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Printed:
4/28/2010 5:45:27 PM
General Analysis Settings
Number of Available Sample and Ladder Wells (Max.) : 12 Minimum Visible Range [s] : 32 Maximum Visible Range [s] : 138 Start Analysis Time Range [s] : 33 End Analysis Time Range [s] : 137.5 Ladder Concentration [pg/µl] : 1950 Uses Standard Area for Ladder Fragments Lower Marker Concentration [pg/µl] : 125 Upper Marker Concentration [pg/µl] : 75 Used Upper Marker for Quantitation Standard Curve Fit is Point to Point Show Data Aligned to Lower and Upper Marker
Integrator Settings
Integration Start Time [s] : 33.05 Integration End Time [s] : 137 Slope Threshold : 0.8 Height Threshold [FU] : 5 Area Threshold : 0.1 Width Threshold [s] : 0.6 Baseline Plateau [s] : 0.5
Filter Settings
Filter Width [s] : 0.5 Polynomial Order : 4
Ladder
Ladder Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Size
35 50 100 150 200 300 400 500 600 700 1000 2000 3000 7000 10380
2100 Expert (B.02.07.SI532)
Area 160 210 208 221 242 270 305 306 336 321 366 413 411 400 214
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
Page
7 of 19
Created: Modified:
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Printed:
4/28/2010 5:45:27 PM
25min
Overall Results for sample 3 : Number of peaks found:
30
Noise:
0.5
Peak table for sample 3 : Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26
Size [bp] 35 57 66 81 97 108 165 183 212 248 270 281 296 307 353 402 414 455 471 488 558 577 629 651 689 735
2100 Expert (B.02.07.SI532)
25min Corr. Area 1:
25min
Conc. [pg/µl] 125.00 277.12 123.26 101.42 63.57 84.07 431.11 157.75 183.94 376.96 177.73 117.63 88.47 107.80 410.77 312.87 94.77 320.57 133.37 159.30 493.40 146.80 315.82 137.77 212.88 182.03
Molarity [pmol/l] 5,411.2 7,313.3 2,814.5 1,904.8 995.3 1,181.6 3,947.3 1,305.6 1,314.0 2,303.0 998.2 633.8 453.0 532.0 1,762.9 1,178.9 346.7 1,066.7 429.2 494.7 1,339.8 385.5 761.2 320.8 468.5 375.5
5,743.5
Observations Lower Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Created: Modified:
Electropherogram Summary Continued ... ... Peak table for sample 3 : Peak 27 28 29 30 31 32
Size [bp] 838 1,145 1,271 1,334 1,428 10,380
Region table for sample 3 : From [bp] 200
To [bp] Corr. Area 1,000 5,743.5
2100 Expert (B.02.07.SI532)
25min
Conc. [pg/µl] 321.92 681.92 156.61 189.12 1,253.62 75.00
% of Total 60
25min
Average Size [bp] 539
Molarity [pmol/l] 582.2 902.5 186.7 214.8 1,329.7 10.9
Size distribution in CV [%] 39.7
Page
8 of 19
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Observations
Upper Marker Conc. [pg/µl] 5,301.65
Molarity [pmol/l] 19,240.0
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
Page
9 of 19
Created: Modified:
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Printed:
4/28/2010 5:45:27 PM
20min
Overall Results for sample 4 : Number of peaks found:
30
Noise:
0.3
Peak table for sample 4 : Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26
Size [bp] 35 43 57 66 75 80 97 168 188 236 253 262 272 296 308 327 337 351 356 375 397 412 504 544 625 668
2100 Expert (B.02.07.SI532)
20min Corr. Area 1:
20min
Conc. [pg/µl] 125.00 71.49 127.61 72.82 49.89 100.76 52.55 223.09 136.71 275.82 131.83 59.71 74.00 194.06 74.32 145.41 78.46 89.09 76.00 75.68 110.72 100.64 384.18 188.98 384.93 226.31
Molarity [pmol/l] 5,411.3 2,490.1 3,375.3 1,663.3 1,008.8 1,899.8 820.0 2,008.6 1,100.4 1,767.6 788.0 344.9 411.9 993.8 365.2 673.8 352.9 385.0 323.3 306.0 422.4 369.7 1,153.9 526.4 933.6 513.5
5,378.9
Observations Lower Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Created: Modified:
Electropherogram Summary Continued ... ... Peak table for sample 4 : Peak 27 28 29 30 31 32
Size [bp] 1,107 1,417 1,561 1,640 10,380 12,592
Region table for sample 4 : From [bp] 200
To [bp] Corr. Area 1,000 5,378.9
2100 Expert (B.02.07.SI532)
20min
Conc. [pg/µl] 1,226.29 537.24 225.53 1,542.78 75.00 0.00
% of Total 52
20min
Average Size [bp] 569
Molarity [pmol/l] 1,679.1 574.3 218.9 1,425.0 10.9 0.0
Size distribution in CV [%] 38.2
Page 10 of 19 4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Observations
Upper Marker
Conc. [pg/µl] 4,369.05
Molarity [pmol/l] 14,964.2
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
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15min
Overall Results for sample 5 : Number of peaks found:
22
Noise:
0.4
Peak table for sample 5 : Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Size [bp] 35 58 67 82 91 99 142 171 197 213 241 255 268 279 294 354 369 395 485 1,655 1,928 10,380 13,489 14,663
2100 Expert (B.02.07.SI532)
15min Corr. Area 1:
15min
Conc. [pg/µl] 125.00 114.02 45.27 96.19 36.73 36.35 58.50 29.95 83.07 56.04 45.34 56.23 57.91 51.32 68.74 271.60 44.00 53.94 337.32 2,219.66 1,565.82 75.00 0.00 0.00
Molarity [pmol/l] 5,411.3 3,003.9 1,022.4 1,784.4 612.9 558.9 622.4 265.1 638.2 398.2 285.1 334.3 327.8 278.4 354.0 1,161.8 180.6 207.0 1,054.5 2,031.6 1,230.2 10.9 0.0 0.0
4,907.2
Observations Lower Marker
Upper Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Created: Modified:
Electropherogram Summary Continued ... ... Region table for sample 5 : From [bp] 200
To [bp] Corr. Area 1,000 4,907.2
2100 Expert (B.02.07.SI532)
% of Total 41
15min
Average Size [bp] 592
Size distribution in CV [%] 36.9
Conc. [pg/µl] 2,942.36
Page 12 of 19
Molarity [pmol/l] 9,653.8
© Copyright 2003 - 2009 Agilent Technologies, Inc.
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
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10min
Overall Results for sample 6 : Number of peaks found:
24
Noise:
0.3
Peak table for sample 6 : Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26
Size [bp] 35 57 67 82 108 157 173 196 214 224 233 248 264 273 298 316 365 381 423 492 1,987 2,265 2,619 10,380 16,087 17,911
2100 Expert (B.02.07.SI532)
10min Corr. Area 1:
10min
Conc. [pg/µl] 125.00 4.19 6.81 14.36 19.70 5.94 4.82 4.74 8.66 5.02 4.53 8.77 6.67 9.56 9.94 14.29 53.19 17.46 37.96 81.96 1,280.20 150.97 1,008.52 75.00 0.00 0.00
Molarity [pmol/l] 5,411.2 111.2 153.6 265.9 277.2 57.2 42.3 36.7 61.2 33.9 29.4 53.6 38.2 53.1 50.5 68.6 220.6 69.4 136.1 252.4 976.0 101.0 583.4 10.9 0.0 0.0
3,244.2
Observations Lower Marker
Upper Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Created: Modified:
Electropherogram Summary Continued ... ... Region table for sample 6 : From [bp] 200
To [bp] Corr. Area 1,000 3,244.2
2100 Expert (B.02.07.SI532)
% of Total 27
10min
Average Size [bp] 616
Size distribution in CV [%] 35.8
Conc. [pg/µl] 1,130.80
Page 14 of 19
Molarity [pmol/l] 3,570.1
© Copyright 2003 - 2009 Agilent Technologies, Inc.
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Page 15 of 19 Created: Modified:
Electropherogram Summary Continued ...
4/28/2010 5:13:27 PM 4/28/2010 5:43:28 PM
0min
Overall Results for sample 7 : Number of peaks found:
6
Noise:
0.3
Peak table for sample 7 : Peak 1 2 3 4 5 6 7 8
Conc. [pg/µl]
Region table for sample 7 : From [bp] 200
To [bp] Corr. Area 1,000 0.0
2100 Expert (B.02.07.SI532)
Corr. Area 1:
0min
Size [bp] 35 6,394 9,205 10,380 17,254 21,426 32,412 33,176
0min
125.00 13.74 23.36 75.00 0.00 0.00 0.00 0.00
% of Total 0
0min
Average Size [bp] 0
Molarity [pmol/l] 5,411.2 3.3 3.8 10.9 0.0 0.0 0.0 0.0
Size distribution in CV [%] 0.0
0.0
Observations Lower Marker Upper Marker
Conc. [pg/µl] 0.00
Molarity [pmol/l] 0.0
© Copyright 2003 - 2009 Agilent Technologies, Inc.
Co lor
Printed:
4/28/2010 5:45:27 PM
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
Electropherogram Summary Continued ...
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Ladder
Setpoint Deviations for sample 12 : Height Threshold [FU] :
20
Area Threshold :
0
Ladder Baseline Plateau [s] :
0.3
Overall Results for Ladder Noise:
0.3
Peak table for Ladder Peak 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Size [bp] 35 50 100 150 200 300 400 500 600 700 1,000 2,000 3,000 7,000 10,380
2100 Expert (B.02.07.SI532)
Conc. [pg/µl] 125.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 150.00 75.00
Molarity [pmol/l] 5,411.3 4,545.5 2,272.7 1,515.2 1,136.4 757.6 568.2 454.5 378.8 324.7 227.3 113.6 75.8 32.5 10.9
Observations Lower Marker Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Ladder Peak Upper Marker
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
Gel Image
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
2100 Expert (B.02.07.SI532)
© Copyright 2003 - 2009 Agilent Technologies, Inc.
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2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
Curves
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad
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Standard Curve
2100 Expert (B.02.07.SI532)
© Copyright 2003 - 2009 Agilent Technologies, Inc.
2100 expert_High Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Assay Class: Data Path:
Run Logbook Description
Page 19 of 19
High Sensitivity DNA Assay C:\...gh Sensitivity DNA Assay_DE72901887_2010-04-28_17-13-27.xad Category
Run started on port 5 (File: C:\Program Files\Agilent\21 00 bioanalyzer\210 0 expert\Data\201 0-04-28\2100 expert_High Sensitivity DNA Assay_DE72901 887_2010-04-28 _17-13-27.xad)
Instrument
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Product Number : G2939A
Instrument
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Name :
Instrument
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Vendor : Agilent Technologies
Instrument
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Serial# : DE72901887
Instrument
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Firmware : C.01.069
Instrument
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Cartridge : Electrode
Instrument
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Instrument
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Time Zone
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Source
Run ended on port 5 (Number of wells acquired: 8)
Number
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