Haiying Li1, Tristan I. Evans1, Michelle Connole1, Jacqueline Gillis1
Acute SIV infection induces hypercytotoxicity and massive numerical loss of innate lymphoid cells in the gastrointestinal tract
R. Keith Reeves, Ph.D. HarNew England Primate Research Center Division of vard Medical School Immunology One Pine Hill Drive Southborough, MA 01772 USA Tel: (508)-786-1429 Fax: (508)-624-8172 Mail: [email protected]
Haiying Li1, Tristan I. Evans1, Michelle Connole1, Jacqueline Gillis1, Fay E. Wong1, Yi Yu1, Laura E. Richert-Spuhler2, Nicole R. Klatt2, R. Keith Reeves1 1Division
of Immunology, NEPRC, Harvard Medical School, Southborough, MA 2Department of Pharmaceutics, Washington National Primate Research Center, University of Washington, Seattle, WA
Acute SIV infection induces cytotoxic and multifunctional subpopulations of NKp44+ ILCs
Rapid and tissue-specific depletion of NKp44+ ILCs in the intestinal mucosae during acute SIV infection
% of live munocuclear cells
0
50K
100K 150K 200K 250K
Naive
D6/7
D14
FSC
Chronic
6 4 2 Naive
D6/7
D14
Naive
NKG2A
HLA-DR 100K 150K 200K 250K
0
FSC
1.0
*
**
0.5 0.2 0.1 0.0 Jejunum
F
8
50K
Jejunum and PaLN
C
10
0
0
Chronic
103
CD3
0
103
104
NKp44
105
Tissue: Colon status: Nave
Tissue: Jejunum status: Nave
Tissue: MLN status: Nave
Tissue: Colon status: acute
Tissue: Jejunum status: acute
Tissue: MLN status: acute
MLN
D
Naive D6/7 D14 Chronic
0.3 0.2
Pie Arcs Pie Chart Arc Legend
0.1 0.0
Naive
D6/7
D14
Chronic
C + CD107a F + IFN-
IL-17 I + T + TNF-
G
2 1
Jejunum
105
PaLN
3
0
104
Acute
105
*
**
0
Chronic
% of live munocuclear cells
NKp44+ ILCs NKG2A+ NK cells
2.5 2.0 1.5 1.0 1.0 0.8 0.6 0.4 0.2 0.0
PaLN
0
Colon
B
103 102
0
MLN
A
PaLN
B
4
+
100.0
1 0
Naive
D6/7
D14
Chronic
Figure1. Depletion of NKp44+ ILCs but not NKG2A+ NK cells in intestinal mucosae during SIV infection. (A) Representative gating strategy to identify NKG2A+ NK cells and NKp44+ ILCs among mononuclear cells in mucosal tissues. Frequencies of NKp44+ILCs among mononuclear cells in colon (B), jejunum, PaLN (C), and MLN (D) were compared between naïve, and acutely and chronically SIV-infected rhesus macaques. Frequencies of NKG2A+ NK cells among mononuclear cells in colon (E), jejunum, PaLN (F), and MLN (G) were also evaluated. *, p
R. Keith Reeves, Ph.D. HarNew England Primate Research Center Division of vard Medical School Immunology One Pine Hill Drive Southborough, MA 01772 USA Tel: (508)-786-1429 Fax: (508)-624-8172 Mail: [email protected]
Haiying Li1, Tristan I. Evans1, Michelle Connole1, Jacqueline Gillis1, Fay E. Wong1, Yi Yu1, Laura E. Richert-Spuhler2, Nicole R. Klatt2, R. Keith Reeves1 1Division
of Immunology, NEPRC, Harvard Medical School, Southborough, MA 2Department of Pharmaceutics, Washington National Primate Research Center, University of Washington, Seattle, WA
Acute SIV infection induces cytotoxic and multifunctional subpopulations of NKp44+ ILCs
Rapid and tissue-specific depletion of NKp44+ ILCs in the intestinal mucosae during acute SIV infection
% of live munocuclear cells
0
50K
100K 150K 200K 250K
Naive
D6/7
D14
FSC
Chronic
6 4 2 Naive
D6/7
D14
Naive
NKG2A
HLA-DR 100K 150K 200K 250K
0
FSC
1.0
*
**
0.5 0.2 0.1 0.0 Jejunum
F
8
50K
Jejunum and PaLN
C
10
0
0
Chronic
103
CD3
0
103
104
NKp44
105
Tissue: Colon status: Nave
Tissue: Jejunum status: Nave
Tissue: MLN status: Nave
Tissue: Colon status: acute
Tissue: Jejunum status: acute
Tissue: MLN status: acute
MLN
D
Naive D6/7 D14 Chronic
0.3 0.2
Pie Arcs Pie Chart Arc Legend
0.1 0.0
Naive
D6/7
D14
Chronic
C + CD107a F + IFN-
IL-17 I + T + TNF-
G
2 1
Jejunum
105
PaLN
3
0
104
Acute
105
*
**
0
Chronic
% of live munocuclear cells
NKp44+ ILCs NKG2A+ NK cells
2.5 2.0 1.5 1.0 1.0 0.8 0.6 0.4 0.2 0.0
PaLN
0
Colon
B
103 102
0
MLN
A
PaLN
B
4
+
100.0
1 0
Naive
D6/7
D14
Chronic
Figure1. Depletion of NKp44+ ILCs but not NKG2A+ NK cells in intestinal mucosae during SIV infection. (A) Representative gating strategy to identify NKG2A+ NK cells and NKp44+ ILCs among mononuclear cells in mucosal tissues. Frequencies of NKp44+ILCs among mononuclear cells in colon (B), jejunum, PaLN (C), and MLN (D) were compared between naïve, and acutely and chronically SIV-infected rhesus macaques. Frequencies of NKG2A+ NK cells among mononuclear cells in colon (E), jejunum, PaLN (F), and MLN (G) were also evaluated. *, p
