HLA Twin Workshop ASHI 2014

Agenda   § 

Introduc)on  to  

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Hands  on  workflows  in  HLA  Twin  (15  mins)   §  High  Quality  Holotype  HLA  X4  data   §  Novel  alleles  with  IMGT/HLA  v3.10   §  Export  consensus  

§  Novel  alleles  with  IMGT/HLA  v3.15   § 

Q&A!  (10  mins)  

 (5  mins)  

Omixon  HLA  Twin™  (So4ware)   § 

SoPware  component  of  Holotype  HLA  

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Automated  genotyping  aPer  MiSeq  run  

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Preconfigured  Holotype  HLA-­‐specific  seVngs  

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Two  algorithms  for  determining  HLA  genotypes   –  Consensus  Genotyping  (Assembly)     –  Sta)s)cal  Genotyping  (Alignment  to  IMGT/HLA)  

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Traffic  light  system  for  data  interpreta)on  and  workflow   management   PASSED   WARNING   FAILED  

3  IT  Implementa