In Vitro and In Vivo Tumor Targeted Photothermal Cancer Therapy ...
Supporting Information
In Vitro and In Vivo Tumor Targeted Photothermal Cancer Therapy Using Functionalized Graphene Nanoparticles Sung Han Kim,1, a Jung Eun Lee,2, a Shazid Md. Sharker,3 Ji Hoon Jeong,2 Insik In,1, 4 Sung Young Park 1
1, 5
*
Department of IT Convergence, Korea National University of Transportation, Chungju 380-
702, Republic of Korea. 2
School of Pharmacy, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon,
Gyeonggi-do 440-746, Republic of Korea. 3
Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST),
Daejeon 305-701, Republic of Korea. 4
Department of Polymer Science and Engineering, Korea National University of
Transportation, Chungju 380-702, Republic of Korea. 5
Department of Chemical and Biological Engineering, Korea National University of
Transportation, Chungju 380-702, Republic of Korea. a
This authors contribute equally to this work
*Corresponding authors Prof. Sung Young Park, E-mail: [email protected]
S1
Figure S1. Synthesis scheme of the preparation of PEG-g-DMA-IR825 (I/C-PgP) composite materials.
S2
Figure S2. The 1H NMR spectra (400 MHz, d6-DMSO) of PEG-g-PDMA-IR825 (PgP/HArGO).
S3
Figure S3. The (a) UV-vis absorption, (b) fluorescence emission and (c) photothermal conversion study of different ratio of D-HA and I/C-PgP-IR825 on the rGO plane (PgP/HArGO). The ratio of D-HA and I/C-PgP on rGO was 20/100/1, 50/50/1 and 80/20/1, respectively. The used sample was 1 mg/mL and NIR laser 808 nm, 2 W/cm2.
S4
Figure S4. The photothermal heat (∆T=Tn-T0) generation curve of C-PgP with rGO as a function of irradiation time. The NIR laser was at 808 nm with the power density of 2 W/cm2.
S5
Figure S5. The photothermal characteristic of heat (∆T=Tn-T0) generation curve of free a) IR825, b) rGO and c) GO as a function of irradiation time. The reduce graphene oxide (rGO) were prepared by well known hydrazine reduction method. The inset shows the photographic image of prepared 1 mg/mL, 0.5 mg/mL and 0.1 mg/mL sample, respectively. The NIR laser was at 808 nm with the power density of 2 W/cm2.
S6
Figure S6. XPS measurements of a) survey scan spectrum, b) C1s and c) N1s narrow scale scan of PgP/HA-rGO.
S7
Figure S7. The comparison of thermograms (TGA) of the PgP/HA-rGO and graphene oxide (GO) as a function weight loss and temperature (ºC).
S8
Figure S8. a) X-ray diffraction (2θ) patterns of GO, I/C-PgP and PgP/HA-rGO, respectively. b) The Raman spectrum for D/G band intensity of GO and PgP/HA-rGO. c) The FTIR transmission peaks of I/C-PgP and PgP/HA-rGO indicating carbonyl peak at 1730 cm-1.
S9
Figure S9. Dispersibility and time dependent colloidal stability at 1 mg/mL of PgP/HA-rGO in (a) serum solution (10 %), and (b) saline solution (0.9 % NaCl), respectively. The inset shows the absorbance at 808 nm wavelength versus time and photographic image between 0 hr and 48 hr.
S 10
Figure S10. Confocal laser scan microscope (CLSM) image of MDAMB-231 cells incubated with PgP/HA-rGO for 0 min, 30 min, 1 hr, 2 hr, 4 hr and 6 hr in neutral pH. (Blue, Lysotracker; Green, PgP/HA-rGO). The fluorescence intensity increased with time owing to endosomal acidic environment. However the increased intensity of PgP/HA-rGO and weak overlap fluorescence of LysoTracker after 4 hr incubation, indicating escape from lysosome. This experiment was performed in neutral pH.
S 11
In Vitro and In Vivo Tumor Targeted Photothermal Cancer Therapy Using Functionalized Graphene Nanoparticles Sung Han Kim,1, a Jung Eun Lee,2, a Shazid Md. Sharker,3 Ji Hoon Jeong,2 Insik In,1, 4 Sung Young Park 1
1, 5
*
Department of IT Convergence, Korea National University of Transportation, Chungju 380-
702, Republic of Korea. 2
School of Pharmacy, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon,
Gyeonggi-do 440-746, Republic of Korea. 3
Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST),
Daejeon 305-701, Republic of Korea. 4
Department of Polymer Science and Engineering, Korea National University of
Transportation, Chungju 380-702, Republic of Korea. 5
Department of Chemical and Biological Engineering, Korea National University of
Transportation, Chungju 380-702, Republic of Korea. a
This authors contribute equally to this work
*Corresponding authors Prof. Sung Young Park, E-mail: [email protected]
S1
Figure S1. Synthesis scheme of the preparation of PEG-g-DMA-IR825 (I/C-PgP) composite materials.
S2
Figure S2. The 1H NMR spectra (400 MHz, d6-DMSO) of PEG-g-PDMA-IR825 (PgP/HArGO).
S3
Figure S3. The (a) UV-vis absorption, (b) fluorescence emission and (c) photothermal conversion study of different ratio of D-HA and I/C-PgP-IR825 on the rGO plane (PgP/HArGO). The ratio of D-HA and I/C-PgP on rGO was 20/100/1, 50/50/1 and 80/20/1, respectively. The used sample was 1 mg/mL and NIR laser 808 nm, 2 W/cm2.
S4
Figure S4. The photothermal heat (∆T=Tn-T0) generation curve of C-PgP with rGO as a function of irradiation time. The NIR laser was at 808 nm with the power density of 2 W/cm2.
S5
Figure S5. The photothermal characteristic of heat (∆T=Tn-T0) generation curve of free a) IR825, b) rGO and c) GO as a function of irradiation time. The reduce graphene oxide (rGO) were prepared by well known hydrazine reduction method. The inset shows the photographic image of prepared 1 mg/mL, 0.5 mg/mL and 0.1 mg/mL sample, respectively. The NIR laser was at 808 nm with the power density of 2 W/cm2.
S6
Figure S6. XPS measurements of a) survey scan spectrum, b) C1s and c) N1s narrow scale scan of PgP/HA-rGO.
S7
Figure S7. The comparison of thermograms (TGA) of the PgP/HA-rGO and graphene oxide (GO) as a function weight loss and temperature (ºC).
S8
Figure S8. a) X-ray diffraction (2θ) patterns of GO, I/C-PgP and PgP/HA-rGO, respectively. b) The Raman spectrum for D/G band intensity of GO and PgP/HA-rGO. c) The FTIR transmission peaks of I/C-PgP and PgP/HA-rGO indicating carbonyl peak at 1730 cm-1.
S9
Figure S9. Dispersibility and time dependent colloidal stability at 1 mg/mL of PgP/HA-rGO in (a) serum solution (10 %), and (b) saline solution (0.9 % NaCl), respectively. The inset shows the absorbance at 808 nm wavelength versus time and photographic image between 0 hr and 48 hr.
S 10
Figure S10. Confocal laser scan microscope (CLSM) image of MDAMB-231 cells incubated with PgP/HA-rGO for 0 min, 30 min, 1 hr, 2 hr, 4 hr and 6 hr in neutral pH. (Blue, Lysotracker; Green, PgP/HA-rGO). The fluorescence intensity increased with time owing to endosomal acidic environment. However the increased intensity of PgP/HA-rGO and weak overlap fluorescence of LysoTracker after 4 hr incubation, indicating escape from lysosome. This experiment was performed in neutral pH.
S 11
