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LECTURE: FERMENTATION KIRSTIN BROWN

Lecture: FERMENTATION Outline: 1. Review of Glycolysis o 2 fates of pyruvate

2. Fermentation o Alcohol fermentation o Lactic acid fermentation

Glycolysis Review o The step-by-step breakdown (oxidation) of glucose to ____________ 1 glucose

1. Review of glycolysis o

2. Fermentation o

Alcohol fermentation

o

Lactic acid fermentation

ATP

ATP

Investment phase Fructose-1-6-bisphosphate

G3P

NAD+

DHAP

G3P

NAD+

NADH

NADH

ADP

ADP

ATP

ATP

Payoff phase

Pyruvate

ADP

ADP

ATP

ATP Pyruvate

Total energy so far: +2 ATP, 2 NADH

2 fates of pyruvate

Glycolysis Review

1. Review of glycolysis

o Pyruvate has 2 possible fates: ➢ Citric acid cycle: when oxygen is present

(_______________) ➢ Fermentation: when oxygen is absent (_______________) Pyruvate O2 Citric acid cycle

No O2 Fermentation

Total energy so far: +2 ATP, 2 NADH

o

2 fates of pyruvate

2. Fermentation o

Alcohol fermentation

o

Lactic acid fermentation

Fermentation Occurs in the absence of oxygen o Purpose: to oxidize NADH back to NAD+ so that it can be recycled in another round of glycolysis o Two types: ➢ Ethanol fermentation: prokaryotes and

yeast. Product is ________________. ➢ Lactic acid fermentation: mammals. Product is ______________. Pyruvate

Pyruvate

CO2 NADH NAD+ NADH NAD+

Ethanol

Lactate

Total energy so far: +2 ATP, ATP 2 NADH

1. Review of glycolysis o

2 fates of pyruvate

2. Fermentation o

Ethanol fermentation

o

Lactic acid fermentation

Fermentation NAD+ is recycled o NAD+ is required to _________________________

1. Review of glycolysis o

2 fates of pyruvate

2. Fermentation

Pyruvate

Pyruvate CO2

NADH NAD+ NADH NAD+

Ethanol

Total energy so far: +2 ATP

Lactate

o

Ethanol fermentation

o

Lactic acid fermentation