Microscope HW AWS

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Microscope HW

1. The diagram below shows how a coverslip should be lowered onto some single-celled organisms during the preparation of a wet mount.

5. A student prepared a slide of pollen grains from a flower. First the pollen was viewed through the low-power objective lens and then, without moving the slide, viewed through the high- power objective lens of a compound light microscope. Which statement best describes the relative number and appearance of the pollen grains observed using these two objectives?

Why is this a preferred procedure? A) The coverslip will prevent the slide from breaking. B) The organisms will be more evenly distributed. C) The possibility of breaking the coverslip is reduced. D) The possibility of trapping air bubbles is reduced. Base your answers to questions 2 through 4 on the diagram of a microscope below and on your knowledge of biology.

A) low power: 25 small pollen grains; high power: 100 large pollen grains B) low power: 100 small pollen grains; high power: 25 large pollen grains C) low power: 25 large pollen grains; high power: 100 small pollen grains D) low power: 100 large pollen grains; high power: 25 small pollen grains 6. A student used the low-power objective of a compound light microscope and observed a single-celled organism as shown in the diagram below.

When he switched to high power, the organism was no longer visible. This most likely happened because switching to high power made the A) B) C) D) 2. Which lettered part should be used to focus the image while using high power? 3. Information about which two lettered parts is needed in order to determine the total magnification of an object viewed with the microscope in the position shown? 4. State two ways the image seen through the microscope differs from the actual specimen being observed.

field too bright to see the organism image too small to be seen area of the slide being viewed smaller fine-adjustment knob no longer functional

Microscope HW 7. A coverslip should be slowly lowered from a 45° angle onto a slide in order to A) prevent the slide from being scratched B) stop the loss of water from under the coverslip C) ensure that the specimen being viewed will stay alive D) reduce the formation of air bubbles 8. Base your answer to the following question on the information and diagrams below and on your knowledge of biology. The drawings below were made during a laboratory exercise in which a microscope was used to view slides of preserved protozoa. The microscope had a 10x eyepiece and two different objectives.

Which statement about the size of the organisms is correct? A) Organism A is larger than organism B. B) Organism B is larger than organism A. C) Organisms A and B are both the same size. D) The relative size of the organisms cannot be determined from the information given. 9. The diagram below represents a specimen in the low-power field of view of a compound light microscope.

If the slide is not moved, which view best represents the way the specimen will look when the high-power objective lens is switched into place? A)

B)

C)

D)