Modular fabrication of polymer brush coated magnetic nanoparticles ...
SUPPORTING INFORMATION
Modular fabrication of polymer brush coated magnetic nanoparticles: Engineering the interface for targeted cellular imaging
Yavuz Oz,a Mehmet Arslan,a Tugce Nihal Gevrek,a Rana Sanyala,b and Amitav Sanyal*a,b a
b
Department of Chemistry, Bogazici University, Bebek, 34342, Istanbul, Turkey
Center for Life Sciences and Technologies, Bogazici University, Istanbul, Turkey
E-mail: [email protected]
S-1
Figure S1 1H NMR spectrum of Dopa-CTA.
Figure S2 13C NMR spectrum of Dopa-CTA.
S-2
OH
Cl
+
H2O
NaN3
O HO
N NC
OH
N3
CN
OH
N O
OH
N3
reflux, 24h 95%
O EDC, DMAP
N3
O
DCM, 24h 48%
N NC
2
azobis-N3
Scheme S1 Synthesis of azobis-N3.
Figure S3 1H NMR spectrum of azobis-N3.
Figure S4 13C NMR spectrum of azobis-N3.
S-3
Figure S5 Field dependent magnetization curve of Fe3O4@OA MNPs.
Figure S6 Images of Fe3O4@PEGMEA MNPs in dilute water dispersion (a) without permanent magnet attraction, (b) with attraction < 10 min and (c) 12 h magnet attraction.
S-4
Figure S7 DLS analysis of iron oxide MNPs a) Fe3O4@OA, b) Fe3O4@CTA and c) Fe3O4@PEGMEA.
S-5
Figure S8 TEM images of magnetic NPs a) Fe3O4@OA, b) Fe3O4@CTA, c) Fe3O4@PEGMEA.
S-6
Figure S9 UV visible spectra of MNPs before and after radical exchange reaction (a) azide modified MNPs and (b) protected maleimide modified MNPs.
Figure S10 DLS analysis of MNPs a) Fe3O4@PEGMEA@pMAL3, b) Fe3O4@PEGMEA@MAL3, c) Fe3O4@PEGMEA@N3.
S-7
Figure S11 TEM images of magnetic NPs a) Fe3O4@PEGMEA@pMAL3, b) Fe3O4@PEGMEA@N3 and c) Fe3O4@PEGMEA@MAL3.
S-8
Figure S12 Absorption spectra after BODIPY conjugation onto maleimide functionalized iron oxide MNPs.
a)
b)
Figure S13 Fluorescence spectra of (a) BODIPY-SH, (b) BODIPY and BODIPY-cRGDfC conjugated MNPs.
S-9
Figure S14 a) UV-vis spectrum of BCA assay standards and cRGD conjugated MNPs b) BCA Assay calibration curve.
Bicinchoninic acid (BCA) protein quantification.
In brief, 100 µl of each standard and MNPs (as unknown sample) were pipetted to glass tubes and 2.0 ml of working reagent prepared as described in the manufacturer’s instructions was added to each tube. Tubes were incubated in a water bath at 60 oC for 30 minutes and then cooled to room temperature. The absorbance of each sample was measured by UV-vis at 562 nm within 10 minutes. Finally, total peptide amount was calculated from calibration curve plotted from the protein standards.
S-10
Modular fabrication of polymer brush coated magnetic nanoparticles: Engineering the interface for targeted cellular imaging
Yavuz Oz,a Mehmet Arslan,a Tugce Nihal Gevrek,a Rana Sanyala,b and Amitav Sanyal*a,b a
b
Department of Chemistry, Bogazici University, Bebek, 34342, Istanbul, Turkey
Center for Life Sciences and Technologies, Bogazici University, Istanbul, Turkey
E-mail: [email protected]
S-1
Figure S1 1H NMR spectrum of Dopa-CTA.
Figure S2 13C NMR spectrum of Dopa-CTA.
S-2
OH
Cl
+
H2O
NaN3
O HO
N NC
OH
N3
CN
OH
N O
OH
N3
reflux, 24h 95%
O EDC, DMAP
N3
O
DCM, 24h 48%
N NC
2
azobis-N3
Scheme S1 Synthesis of azobis-N3.
Figure S3 1H NMR spectrum of azobis-N3.
Figure S4 13C NMR spectrum of azobis-N3.
S-3
Figure S5 Field dependent magnetization curve of Fe3O4@OA MNPs.
Figure S6 Images of Fe3O4@PEGMEA MNPs in dilute water dispersion (a) without permanent magnet attraction, (b) with attraction < 10 min and (c) 12 h magnet attraction.
S-4
Figure S7 DLS analysis of iron oxide MNPs a) Fe3O4@OA, b) Fe3O4@CTA and c) Fe3O4@PEGMEA.
S-5
Figure S8 TEM images of magnetic NPs a) Fe3O4@OA, b) Fe3O4@CTA, c) Fe3O4@PEGMEA.
S-6
Figure S9 UV visible spectra of MNPs before and after radical exchange reaction (a) azide modified MNPs and (b) protected maleimide modified MNPs.
Figure S10 DLS analysis of MNPs a) Fe3O4@PEGMEA@pMAL3, b) Fe3O4@PEGMEA@MAL3, c) Fe3O4@PEGMEA@N3.
S-7
Figure S11 TEM images of magnetic NPs a) Fe3O4@PEGMEA@pMAL3, b) Fe3O4@PEGMEA@N3 and c) Fe3O4@PEGMEA@MAL3.
S-8
Figure S12 Absorption spectra after BODIPY conjugation onto maleimide functionalized iron oxide MNPs.
a)
b)
Figure S13 Fluorescence spectra of (a) BODIPY-SH, (b) BODIPY and BODIPY-cRGDfC conjugated MNPs.
S-9
Figure S14 a) UV-vis spectrum of BCA assay standards and cRGD conjugated MNPs b) BCA Assay calibration curve.
Bicinchoninic acid (BCA) protein quantification.
In brief, 100 µl of each standard and MNPs (as unknown sample) were pipetted to glass tubes and 2.0 ml of working reagent prepared as described in the manufacturer’s instructions was added to each tube. Tubes were incubated in a water bath at 60 oC for 30 minutes and then cooled to room temperature. The absorbance of each sample was measured by UV-vis at 562 nm within 10 minutes. Finally, total peptide amount was calculated from calibration curve plotted from the protein standards.
S-10
