Na' and K+ Transport at Basolateral Membranes of ... - Europe PMC

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Na' and K+ Transport at Basolateral Membranes of Epithelial Cells II. K+ Efflux and Stoichiometry of the Na,K-ATPase THOMAS C . COX and SANDY 1 . HELMAN From the Department of Physiology and Biophysics, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801

Changes of "K efflux (JKS) caused by ouabain and/or furosemide "K influx were measured in isolated epithelia of frog skin . From the kinetics of ( JK) studied first over 8-9 h, K+ appeared to be distributed into readily and poorly exchangeable cellular pools of K+. The readily exchangeable pool of K+ was increased by amiloride and decreased by ouabain and/or K''-free extracellular Ringer solution . 42K efflux studies were carried out with tissues shortcircuited in chambers . Ouabain caused an immediate (