Nitrosamine Formation Associated with Barn Rot ... AWS
11/28/2017
Host resistance for managing soilborne diseases in strawberry production Kelly Ivors Horticulture & Crop Science Cal Poly Strawberry Center & Gerald Holmes Cal Poly Strawberry Center
1
11/28/2017
NEXT GENERATION DISEASE RESISTANCE BREEDING
1 = highest priority; 2 = medium priority; 3 = lowest priority; N = non-priority. SD = short-day, DN = day-neutral, and NP = nursery production. R-gene = resistance gene, QTL = large-effect quantitative trait locus, complex = polygenic, complex genetics, and ? = unknown or hypothesized.
2
11/28/2017
Fields infested on the campus of Cal Poly:
Macrophomina phaseolina Verticillium dahliae
3
11/28/2017
Field infested at the Monterey Bay Academy: Fusarium oxysporum f.sp. fragariae
4
11/28/2017
Cal Poly Trials 6 breeding programs 90 genotypes • 30 cultivars • 60 elite selections
5
11/28/2017
Fields infested on the campus of Cal Poly:
Macrophomina phaseolina
6
11/28/2017
MACROPHOMINA TRIAL
March 1, 2017
7
11/28/2017
MACROPHOMINA TRIAL
August 1, 2017
8
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
9
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY Monterey: 69% dead
10
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
Albion: 52% dead
11
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
Portola: 40% dead
12
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
San Andreas: 27% dead
13
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
UF
UC
PS
PL
LC
DR
14
11/28/2017
Fields infested on the campus of Cal Poly:
Macrophomina phaseolina Verticillium dahliae
15
11/28/2017
VERTICILLIUM TRIAL
March 21, 2017
16
11/28/2017
VERTICILLIUM TRIAL
July 15, 2017
17
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
18
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
Monterey: 26% dead
19
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
Albion 10% dead
20
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
San Andreas: 8% dead
21
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
UF
UC
PS
PL
LC
DR
22
11/28/2017
VERTICILLIUM TRIAL 2015
San Andreas
Portola
Albion
Monterey
23
11/28/2017
Field infested at the Monterey Bay Academy: Fusarium oxysporum f.sp. fragariae
24
11/28/2017
FUSARIUM TRIAL 2015
25
11/28/2017
FUSARIUM TRIAL 2015
26
11/28/2017
FUSARIUM TRIAL 2015 March 20, 2015
This is a map of Rep I in the MBA variety trial, where the soilborne fungus Fusarium oxysporum f. sp. fragariae is established. Each area surrounded by black lines represents one bed of a different strawberry cultivar (cultivar name listed at the top of each bed). Light orange color = leaf necrosis; burnt orange color = dead strawberry plant.
27
11/28/2017
FUSARIUM TRIAL 2015 May 15, 2015
This is a map of Rep I in the MBA variety trial, where the soilborne fungus Fusarium oxysporum f. sp. fragariae is established. Each area surrounded by black lines represents one bed of a different strawberry cultivar (cultivar name listed at the top of each bed). Light orange color = leaf necrosis; burnt orange color = dead strawberry plant.
28
11/28/2017
FUSARIUM TRIAL 2015 July 23, 2015
This is a map of Rep I in the MBA variety trial, where the soilborne fungus Fusarium oxysporum f. sp. fragariae is established. Each area surrounded by black lines represents one bed of a different strawberry cultivar (cultivar name listed at the top of each bed). Light orange color = leaf necrosis; burnt orange color = dead strawberry plant.
29
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
20.00
40.00
60.00
80.00
100.00
30
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
Monterey: 75% dead
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
20.00
40.00
60.00
80.00
100.00
31
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
Albion: 53% dead
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
20.00
40.00
60.00
80.00
100.00
32
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
San Andreas: 4% dead
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
20.00
40.00
60.00
80.00
100.00
33
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
Portola: 2% dead
20.00
40.00
60.00
80.00
100.00
34
11/28/2017
CULTIVAR EVALUATION TABLE
35
11/28/2017
CONCLUSIONS High levels of resistance
Wide range of susceptibility in germplasm Strawberry Center to continue host resistance screening for additional diseases…
36
11/28/2017
THANKS TO…
37
Host resistance for managing soilborne diseases in strawberry production Kelly Ivors Horticulture & Crop Science Cal Poly Strawberry Center & Gerald Holmes Cal Poly Strawberry Center
1
11/28/2017
NEXT GENERATION DISEASE RESISTANCE BREEDING
1 = highest priority; 2 = medium priority; 3 = lowest priority; N = non-priority. SD = short-day, DN = day-neutral, and NP = nursery production. R-gene = resistance gene, QTL = large-effect quantitative trait locus, complex = polygenic, complex genetics, and ? = unknown or hypothesized.
2
11/28/2017
Fields infested on the campus of Cal Poly:
Macrophomina phaseolina Verticillium dahliae
3
11/28/2017
Field infested at the Monterey Bay Academy: Fusarium oxysporum f.sp. fragariae
4
11/28/2017
Cal Poly Trials 6 breeding programs 90 genotypes • 30 cultivars • 60 elite selections
5
11/28/2017
Fields infested on the campus of Cal Poly:
Macrophomina phaseolina
6
11/28/2017
MACROPHOMINA TRIAL
March 1, 2017
7
11/28/2017
MACROPHOMINA TRIAL
August 1, 2017
8
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
9
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY Monterey: 69% dead
10
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
Albion: 52% dead
11
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
Portola: 40% dead
12
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
San Andreas: 27% dead
13
11/28/2017
MACROPHOMINA CROWN ROT SUSCEPTIBILITY
UF
UC
PS
PL
LC
DR
14
11/28/2017
Fields infested on the campus of Cal Poly:
Macrophomina phaseolina Verticillium dahliae
15
11/28/2017
VERTICILLIUM TRIAL
March 21, 2017
16
11/28/2017
VERTICILLIUM TRIAL
July 15, 2017
17
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
18
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
Monterey: 26% dead
19
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
Albion 10% dead
20
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
San Andreas: 8% dead
21
11/28/2017
VERTICILLIUM WILT SUSCEPTIBILITY
UF
UC
PS
PL
LC
DR
22
11/28/2017
VERTICILLIUM TRIAL 2015
San Andreas
Portola
Albion
Monterey
23
11/28/2017
Field infested at the Monterey Bay Academy: Fusarium oxysporum f.sp. fragariae
24
11/28/2017
FUSARIUM TRIAL 2015
25
11/28/2017
FUSARIUM TRIAL 2015
26
11/28/2017
FUSARIUM TRIAL 2015 March 20, 2015
This is a map of Rep I in the MBA variety trial, where the soilborne fungus Fusarium oxysporum f. sp. fragariae is established. Each area surrounded by black lines represents one bed of a different strawberry cultivar (cultivar name listed at the top of each bed). Light orange color = leaf necrosis; burnt orange color = dead strawberry plant.
27
11/28/2017
FUSARIUM TRIAL 2015 May 15, 2015
This is a map of Rep I in the MBA variety trial, where the soilborne fungus Fusarium oxysporum f. sp. fragariae is established. Each area surrounded by black lines represents one bed of a different strawberry cultivar (cultivar name listed at the top of each bed). Light orange color = leaf necrosis; burnt orange color = dead strawberry plant.
28
11/28/2017
FUSARIUM TRIAL 2015 July 23, 2015
This is a map of Rep I in the MBA variety trial, where the soilborne fungus Fusarium oxysporum f. sp. fragariae is established. Each area surrounded by black lines represents one bed of a different strawberry cultivar (cultivar name listed at the top of each bed). Light orange color = leaf necrosis; burnt orange color = dead strawberry plant.
29
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
20.00
40.00
60.00
80.00
100.00
30
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
Monterey: 75% dead
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
20.00
40.00
60.00
80.00
100.00
31
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
Albion: 53% dead
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
20.00
40.00
60.00
80.00
100.00
32
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
San Andreas: 4% dead
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
20.00
40.00
60.00
80.00
100.00
33
11/28/2017
FUSARIUM TRIAL 2015 Percent Mortality on July 23, 2015 Sweet Ann
96.5
Monterey
75.7
Radiance
55.2
Albion
53.2
Sabrina
33.3
Grenada
32.3
Sensation
26.7
Winterstar
16.4
Ventana
9.9
San Andreas
4.1
Festival
2.3
Portola
1.7
Safari
0.9
Fronteras
0.6
Petaluma
0.3 0.00
Portola: 2% dead
20.00
40.00
60.00
80.00
100.00
34
11/28/2017
CULTIVAR EVALUATION TABLE
35
11/28/2017
CONCLUSIONS High levels of resistance
Wide range of susceptibility in germplasm Strawberry Center to continue host resistance screening for additional diseases…
36
11/28/2017
THANKS TO…
37
