Nucleotide sequence of the infectious cloned DNA components of ...
.::) Oxford University Press 1990
Nucleic Acids Research, Vol. 18, No. I
Nucleotide
of the infectious cloned DNA components of African cassava mosaic virus (Nigerian strain) sequence
Bret Morris, Leigh Coates, Suzanne Lowe, Kim Richardson and Peter Eddy Molecular Biology Facility, DSIR Plant Protection, Private Bag, Auckland, New Zealand Submitted November 21, 1989
African cassava mosaic virus Nigerian strain (ACMV-N, 1) DNA 1 is 2781 long and contains five open reading frames (ORFs)coding for proteins with Mrl3,131 (13.1K), Mr30,210 (30.2K), Mr4O,435 (40.4K), MrI5,178 (15.2K) and MrI5,937 (15.9K). ACMV-N DNA 2 is 2725 long and contains two ORFs coding for proteins with Mr29,379 (29.4K) and Mr33,678 (33.7K). The ACMV-N DNA 1 sequence 448n to 1227n encoding the 30.2K ORF has been reported (1). Comparison of the ACMV-N nucleotide sequence with ACMV Kenyan strain (ACMV-K, 2) shows 96% homology for DNA 1 and 94% homology for DNA 2. Nucleotide changes from the ACMV-K strain are shown in the upper case with additional nucleotides (+) and deleted nucleotides (-) above the sequence. The 13.1K, acmv-n DNA 1
1 81 161 241 321 401 481 561
641 721 801 881 961 1041 1121 1201 1281 1361 1441
1521 1601 1681 1761 1841 1921 2001
EMBL accession nos X17095, X17096
30.2K, 40.4K, 15.2K, 15.9K, 29.4K and33.7K ORFs contain 6, 11, 6, 4, 9, 19 and 8 amino acid changes respectively when compared to the corresponding ACMV-K ORFs. Plants of Nicotiana benthamiana and N. clevelandii inoculated with combinations of DNA 1 and DNA 2 of ACMV-N and ACMVK excised from their respective clones showed a correlation of more severe symptoms with DNA 2 of ACMV-K. This contrasts with that previously reported (1).
REFERENCES 1. Stanley,J. et al. (1985) J. Gen. Virol. 66, 1055-1061. 2. Stanley,J. and Davies,J.W. (1985) In Plant Molecular Virology, Vol. 2, Davies,J.W. (ed) CRC Press, Boca Raton,
..
ctcaactGgagacacCcttgagcatctcctcctattaattggagacattatatagTtgtctcCaaatggcattcttgtaa taagtGgTactttaatttgaatGaaaaggctcaaaaggcGcagaacacccaaggggccaaccgtataatattaccggttg gccccgcccccCtttaaACgtggtccccgcgcactactCatgtcggccaatcatgCtgtagcGttaaaggttatTtatta gtggtggAccactatataCttAcaggcCaagtCgtTgctagtgcgcaatgtgggatccactggtgaatgagtttccagac tcCgtgcatgggcttaggtgtatgctTgcaattaaatatttgcaggccttagaggatacatacgagcccagtacTttggg ccacgaTctggtTagAgatctaAtctcagttatcagggctcgtaattatgtcgaagcgaccaggagatatcatcatttcc actccaggCtcGaaggttcgtcgaagGctgaacttcgacagcccatacaggaaccgtgctactgcccccactgtccacgt
cacaaatcgaaaacgggcctggAtgaacaggcccatgtacagaaagcccaTgatgtacaggatgtatagaagcccagaca tacctagAggctgtgaaggcccatgtaaggtccagtcgtttgagcagagggatgatgtgaagcaccttggtatctgtaag gtgattagtgatgtgacAcgtgggcctgggctgacacacagAgtcggaaagaggttttgtatcaagtccatttacattct tggtaagatctggAtggatgaaaAtattaagaagcaGaatcacacGaataatgtgatGttttacctgcttagggatagaa ggccTtatggcaatAcgccccaagacttTgggcagatatttaacatgtttgataatgagcccagtactgcaacaatCGag aacgatttgagggataggtttcaggtgttgaggaaatttcatgccactgttAttggtggtcCatCtGgcatgaaggagca ggcgttggtgaaaaggttttacaggttgaatcatcacgtgacatacaatcatcaggaggcagggaagtaCgagaatcaca cagagaatgctttgcttTtgtacatggcatgtactcatgcctccaatcctgtatatgcTacgttgaaaatacgtatatac ttctatgacagtattggcaattaataaacattgaattttatttcatgagtcaactGAcactcaatagtTttTTcaattac attgaacaaaacatgaCAagcagctctaattacatCgttaattgagataacaCctatattatccaagtatttaaGtactt ggtatctaaagacTcttaagaaaagaccagtctgaggcCgtaaggtCgtccagatcCTgaagttgagaaaacatttgtga atccccagctccttcctcaggttgtgattgaatcgaacctggactgttatgatgtcctggttcagcaggaatggtcgttg ttggtgcctggtgattgtgaaatacaggggattgtttatttc caggtatacacgccattcattgcttgaggagcagtga tgaGttcccctgtgcgtaaatccatgattggagcagttgatatggaggtaatatgaacaGccacagacaagatccactct cctacgccggatggctcgcttcttgaAttgtctgtgaCtgactttgattggaacctgagtagagtggttcGgtgagggtg atgaagattgcattctttaatgcccaggcctttagcgcttcttgcttttcctcgTcGaggaactctttataggacgaggt aggtcctggattgcagaggaagatagtgggaatGccacctttaatttgaacgggtttcccgtatttCgtgttggactgcc agtccctctgggaccccatgaattccttaaagtgctttaggtagtggggatcgacgtcatcaatgacgttgtaccaggca TcattattgaagaccttAggactaaggtccaggtgtccacacaggtaattGtgtgggcctaaagatctggcccatatcgt 197
198 Nucleic Acids Research 2081 2161 2241 2321 2401 2481 2561 2641 2721
cttccctgttctgctatcaccttctatTacaatactattGggtctccatggccgcgcagcggaatccctaacattatcag cgacccattcttcaaGttcaTcaggaacttggtcaaaggaagaacatgggaagggagaaacataaggagctggtggctcC tggaaaatcctatctaaattactatttagattatgaaactgaagtacaaagtcctttgggactaattccctaatTacatt aagagcttctgacttactgccgctgttaagcgctttggcgtaagcatcattcgctgattgttgAccgcctctagcagatc gtccatcgatCtgaaattgtccccattcgacggtgtcTccgtccttatccagataggacttgacatcTgagcttgatttg gcaccttgaatgttggggtggaaaTtggtgctacagcttgggtgtacacaatcgaagagacgattgttcgtaatcgtgat CttTccctcgaattgGatgagggcatgcaagtgaggttccccattctgatgcagctctcGacagattttaatgaacttag ggtttgatgggagagaTagtgtttgaatgaatgacagCaggtgttctttgggtatagaacaAtttgggtatgtgagaaag acattcttggcttgaaCtcTaaaacgaggagttcGcatTttgaccaagtcaattggagaca
acmv-n DNA 2 1 81 161 241 321 401 481 561 641 721 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2721
..
ctcaactggagacacTcttgagcatctcctcctattaattggagacattatatagTtgtctcCaaaTggcattcttgtaa
taagtGgaactttaatttGaaTtaaaagTctcaaaaggctcagaacacccaa9gggccaaccgtataatattaccggttg gccccgcccctCttTaaAtgtggTccccgcgcacCggttggcCCcgCcCcTT tttaaagtggICCccCcaCGtGCttcG ctttcTtgcagataTGaTggtggtccccgcatGCagGtgttgtcTgctatggatattgcatgtgtgTcccacatgcaaat aaatGatctAcgggccTatcgaatgacagcCtgtgCtactaaattatatttagtatattgttcatTCctctgagttaata aaaTgGtataGTttaTttaacTGttaatattagccattcgataGtaGtgaGtTatagtcAtcaatctgacaacgaTcatG aaggacaattaaggcGagtagcgtaattgtttatgtattatatatagggtgcTaaCatacaggagttTgagaatatcaGG tatCggaaTtatatacattGATttgtaatgtatagtatcaggaagcagCctagaaatTtccaaaggaaatTTaacagcaa taCcactaacaggtTtccaatCaGgcgtaagtatgttgGTgggcatacTagaccatCtgttaggcgaagattgACatatg agccagtggagagaccgttGgTccataatgttttgtgtgagaagcaacatggtgatgtgtttaatttgcagcagaacaca agttacacgtcAtttgtgacgtatccttccaggggaccatctggtgatgggcgtagtagggattacatcaagttgcaaag tatgtctgtctcgggagtgatacatgcgaaggctaatTgcaatgatgatcctatggaggtgtcTcAtgATgttaatggag tgttcgtgtttagcttaatcatggaCacaaagccttatttaccTgctggtgttcaagCtcttccTacatttgaagagCtg ttcggaGcatattccgcttgttacgttaatttaagattattgaataatcaacagcatcgatatagggtattgcacagcgt gaaacgTttcgtGtcatctGcaggagatactaaggtatcGcaatttagAtttaataagcgattgagcaccaggcgatata AtatTtgggcatcattccatgatggggatctggtaaatgcaggtggaaattataggaacataagcaagaatgctattctt gtcagttatgcttttgtatcagagcatTcaatgtcttgtaagccatttgtacaaatagaaacatcctatgtTggatgatt tattattCataaatctttatggctactGcctctatgtgctattaagcacttgtttacagtttgttctataatgctttcaa tgtcctttttcgTcatggactgggtttgTgaaaCGgagtcccctggatctagggaagcttcaggtagctggtgtagccct ctaagtgggaattcTgcctcagaggCatactgcttggaggatgagtcgtcgatgtcaattTgTtttggGcttgtgtatct catgcttgtaGagcgcccaattgtggattttgtagcccatgtttctcctggttggaccgtgatgggcctatatttaggcc cagtgtcataatctgtgccgggctcattCtgaatgagccttcgaatgggcttaggttttccaacggaccagaagtcCaca cagtcaggcccatagtctttggatagtatttttatagtgggctgtttaaatCtaatgtccgttgaatgtttggcagcGga cagcttCagtttAgccttaatttgggcaaatgtgaCtccattctttacgttGgagtcttcaaccttatacagcagctgcc atggtacgttatcatcaattgaaaagtatgatgcagagaaataatggaggtccacattacaccctatgggaaatgtgaaT tgggcttgatcctgttgctcatcactgagCctagtgtccctaatggtaacGataacagaaccttgtgcattaaagggcac ttgattcctgtaTtcaatgacGacatggtctaccttcatgcatttgcccatgatacgtacccttgttctTtcaagggtcg aaggaaattgaagtgtaatcggcgattcatcattcgtcagtttgtattctgttcttgcggaTtgaatataGtcgctggat atgacaggtacagatgtatccatgttcaacactCtgagtataagettgtcaaagCcacaacacctgtctcatgaTaaaca tgaAtaatctattAtaaaactggccgcgcagcggaatggCttAaaGtcaagataaatcagaagatttgtcaacaggGtaa acagaTtgtGgaaatatagacatatatttcttatcctaCcggaaaaatgcGtctaTgGCtaatccaattcaaacataTat atatgcatacaGcattagtcgatgaaacatGatTtatGtagagatatgtacctGaTaataactGAaattggatatcTGct ggagtggaatgtggatAgtcttattatcaaagtggtcatataaatatgtGttatatatttattaaggagttatccaacga gtgatagtttacgtGgttagatttgcatgttcaaatctaaaataagcaattacaCtatagtgagagaaagttagagagag acgct
Nucleic Acids Research, Vol. 18, No. I
Nucleotide
of the infectious cloned DNA components of African cassava mosaic virus (Nigerian strain) sequence
Bret Morris, Leigh Coates, Suzanne Lowe, Kim Richardson and Peter Eddy Molecular Biology Facility, DSIR Plant Protection, Private Bag, Auckland, New Zealand Submitted November 21, 1989
African cassava mosaic virus Nigerian strain (ACMV-N, 1) DNA 1 is 2781 long and contains five open reading frames (ORFs)coding for proteins with Mrl3,131 (13.1K), Mr30,210 (30.2K), Mr4O,435 (40.4K), MrI5,178 (15.2K) and MrI5,937 (15.9K). ACMV-N DNA 2 is 2725 long and contains two ORFs coding for proteins with Mr29,379 (29.4K) and Mr33,678 (33.7K). The ACMV-N DNA 1 sequence 448n to 1227n encoding the 30.2K ORF has been reported (1). Comparison of the ACMV-N nucleotide sequence with ACMV Kenyan strain (ACMV-K, 2) shows 96% homology for DNA 1 and 94% homology for DNA 2. Nucleotide changes from the ACMV-K strain are shown in the upper case with additional nucleotides (+) and deleted nucleotides (-) above the sequence. The 13.1K, acmv-n DNA 1
1 81 161 241 321 401 481 561
641 721 801 881 961 1041 1121 1201 1281 1361 1441
1521 1601 1681 1761 1841 1921 2001
EMBL accession nos X17095, X17096
30.2K, 40.4K, 15.2K, 15.9K, 29.4K and33.7K ORFs contain 6, 11, 6, 4, 9, 19 and 8 amino acid changes respectively when compared to the corresponding ACMV-K ORFs. Plants of Nicotiana benthamiana and N. clevelandii inoculated with combinations of DNA 1 and DNA 2 of ACMV-N and ACMVK excised from their respective clones showed a correlation of more severe symptoms with DNA 2 of ACMV-K. This contrasts with that previously reported (1).
REFERENCES 1. Stanley,J. et al. (1985) J. Gen. Virol. 66, 1055-1061. 2. Stanley,J. and Davies,J.W. (1985) In Plant Molecular Virology, Vol. 2, Davies,J.W. (ed) CRC Press, Boca Raton,
..
ctcaactGgagacacCcttgagcatctcctcctattaattggagacattatatagTtgtctcCaaatggcattcttgtaa taagtGgTactttaatttgaatGaaaaggctcaaaaggcGcagaacacccaaggggccaaccgtataatattaccggttg gccccgcccccCtttaaACgtggtccccgcgcactactCatgtcggccaatcatgCtgtagcGttaaaggttatTtatta gtggtggAccactatataCttAcaggcCaagtCgtTgctagtgcgcaatgtgggatccactggtgaatgagtttccagac tcCgtgcatgggcttaggtgtatgctTgcaattaaatatttgcaggccttagaggatacatacgagcccagtacTttggg ccacgaTctggtTagAgatctaAtctcagttatcagggctcgtaattatgtcgaagcgaccaggagatatcatcatttcc actccaggCtcGaaggttcgtcgaagGctgaacttcgacagcccatacaggaaccgtgctactgcccccactgtccacgt
cacaaatcgaaaacgggcctggAtgaacaggcccatgtacagaaagcccaTgatgtacaggatgtatagaagcccagaca tacctagAggctgtgaaggcccatgtaaggtccagtcgtttgagcagagggatgatgtgaagcaccttggtatctgtaag gtgattagtgatgtgacAcgtgggcctgggctgacacacagAgtcggaaagaggttttgtatcaagtccatttacattct tggtaagatctggAtggatgaaaAtattaagaagcaGaatcacacGaataatgtgatGttttacctgcttagggatagaa ggccTtatggcaatAcgccccaagacttTgggcagatatttaacatgtttgataatgagcccagtactgcaacaatCGag aacgatttgagggataggtttcaggtgttgaggaaatttcatgccactgttAttggtggtcCatCtGgcatgaaggagca ggcgttggtgaaaaggttttacaggttgaatcatcacgtgacatacaatcatcaggaggcagggaagtaCgagaatcaca cagagaatgctttgcttTtgtacatggcatgtactcatgcctccaatcctgtatatgcTacgttgaaaatacgtatatac ttctatgacagtattggcaattaataaacattgaattttatttcatgagtcaactGAcactcaatagtTttTTcaattac attgaacaaaacatgaCAagcagctctaattacatCgttaattgagataacaCctatattatccaagtatttaaGtactt ggtatctaaagacTcttaagaaaagaccagtctgaggcCgtaaggtCgtccagatcCTgaagttgagaaaacatttgtga atccccagctccttcctcaggttgtgattgaatcgaacctggactgttatgatgtcctggttcagcaggaatggtcgttg ttggtgcctggtgattgtgaaatacaggggattgtttatttc caggtatacacgccattcattgcttgaggagcagtga tgaGttcccctgtgcgtaaatccatgattggagcagttgatatggaggtaatatgaacaGccacagacaagatccactct cctacgccggatggctcgcttcttgaAttgtctgtgaCtgactttgattggaacctgagtagagtggttcGgtgagggtg atgaagattgcattctttaatgcccaggcctttagcgcttcttgcttttcctcgTcGaggaactctttataggacgaggt aggtcctggattgcagaggaagatagtgggaatGccacctttaatttgaacgggtttcccgtatttCgtgttggactgcc agtccctctgggaccccatgaattccttaaagtgctttaggtagtggggatcgacgtcatcaatgacgttgtaccaggca TcattattgaagaccttAggactaaggtccaggtgtccacacaggtaattGtgtgggcctaaagatctggcccatatcgt 197
198 Nucleic Acids Research 2081 2161 2241 2321 2401 2481 2561 2641 2721
cttccctgttctgctatcaccttctatTacaatactattGggtctccatggccgcgcagcggaatccctaacattatcag cgacccattcttcaaGttcaTcaggaacttggtcaaaggaagaacatgggaagggagaaacataaggagctggtggctcC tggaaaatcctatctaaattactatttagattatgaaactgaagtacaaagtcctttgggactaattccctaatTacatt aagagcttctgacttactgccgctgttaagcgctttggcgtaagcatcattcgctgattgttgAccgcctctagcagatc gtccatcgatCtgaaattgtccccattcgacggtgtcTccgtccttatccagataggacttgacatcTgagcttgatttg gcaccttgaatgttggggtggaaaTtggtgctacagcttgggtgtacacaatcgaagagacgattgttcgtaatcgtgat CttTccctcgaattgGatgagggcatgcaagtgaggttccccattctgatgcagctctcGacagattttaatgaacttag ggtttgatgggagagaTagtgtttgaatgaatgacagCaggtgttctttgggtatagaacaAtttgggtatgtgagaaag acattcttggcttgaaCtcTaaaacgaggagttcGcatTttgaccaagtcaattggagaca
acmv-n DNA 2 1 81 161 241 321 401 481 561 641 721 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2721
..
ctcaactggagacacTcttgagcatctcctcctattaattggagacattatatagTtgtctcCaaaTggcattcttgtaa
taagtGgaactttaatttGaaTtaaaagTctcaaaaggctcagaacacccaa9gggccaaccgtataatattaccggttg gccccgcccctCttTaaAtgtggTccccgcgcacCggttggcCCcgCcCcTT tttaaagtggICCccCcaCGtGCttcG ctttcTtgcagataTGaTggtggtccccgcatGCagGtgttgtcTgctatggatattgcatgtgtgTcccacatgcaaat aaatGatctAcgggccTatcgaatgacagcCtgtgCtactaaattatatttagtatattgttcatTCctctgagttaata aaaTgGtataGTttaTttaacTGttaatattagccattcgataGtaGtgaGtTatagtcAtcaatctgacaacgaTcatG aaggacaattaaggcGagtagcgtaattgtttatgtattatatatagggtgcTaaCatacaggagttTgagaatatcaGG tatCggaaTtatatacattGATttgtaatgtatagtatcaggaagcagCctagaaatTtccaaaggaaatTTaacagcaa taCcactaacaggtTtccaatCaGgcgtaagtatgttgGTgggcatacTagaccatCtgttaggcgaagattgACatatg agccagtggagagaccgttGgTccataatgttttgtgtgagaagcaacatggtgatgtgtttaatttgcagcagaacaca agttacacgtcAtttgtgacgtatccttccaggggaccatctggtgatgggcgtagtagggattacatcaagttgcaaag tatgtctgtctcgggagtgatacatgcgaaggctaatTgcaatgatgatcctatggaggtgtcTcAtgATgttaatggag tgttcgtgtttagcttaatcatggaCacaaagccttatttaccTgctggtgttcaagCtcttccTacatttgaagagCtg ttcggaGcatattccgcttgttacgttaatttaagattattgaataatcaacagcatcgatatagggtattgcacagcgt gaaacgTttcgtGtcatctGcaggagatactaaggtatcGcaatttagAtttaataagcgattgagcaccaggcgatata AtatTtgggcatcattccatgatggggatctggtaaatgcaggtggaaattataggaacataagcaagaatgctattctt gtcagttatgcttttgtatcagagcatTcaatgtcttgtaagccatttgtacaaatagaaacatcctatgtTggatgatt tattattCataaatctttatggctactGcctctatgtgctattaagcacttgtttacagtttgttctataatgctttcaa tgtcctttttcgTcatggactgggtttgTgaaaCGgagtcccctggatctagggaagcttcaggtagctggtgtagccct ctaagtgggaattcTgcctcagaggCatactgcttggaggatgagtcgtcgatgtcaattTgTtttggGcttgtgtatct catgcttgtaGagcgcccaattgtggattttgtagcccatgtttctcctggttggaccgtgatgggcctatatttaggcc cagtgtcataatctgtgccgggctcattCtgaatgagccttcgaatgggcttaggttttccaacggaccagaagtcCaca cagtcaggcccatagtctttggatagtatttttatagtgggctgtttaaatCtaatgtccgttgaatgtttggcagcGga cagcttCagtttAgccttaatttgggcaaatgtgaCtccattctttacgttGgagtcttcaaccttatacagcagctgcc atggtacgttatcatcaattgaaaagtatgatgcagagaaataatggaggtccacattacaccctatgggaaatgtgaaT tgggcttgatcctgttgctcatcactgagCctagtgtccctaatggtaacGataacagaaccttgtgcattaaagggcac ttgattcctgtaTtcaatgacGacatggtctaccttcatgcatttgcccatgatacgtacccttgttctTtcaagggtcg aaggaaattgaagtgtaatcggcgattcatcattcgtcagtttgtattctgttcttgcggaTtgaatataGtcgctggat atgacaggtacagatgtatccatgttcaacactCtgagtataagettgtcaaagCcacaacacctgtctcatgaTaaaca tgaAtaatctattAtaaaactggccgcgcagcggaatggCttAaaGtcaagataaatcagaagatttgtcaacaggGtaa acagaTtgtGgaaatatagacatatatttcttatcctaCcggaaaaatgcGtctaTgGCtaatccaattcaaacataTat atatgcatacaGcattagtcgatgaaacatGatTtatGtagagatatgtacctGaTaataactGAaattggatatcTGct ggagtggaatgtggatAgtcttattatcaaagtggtcatataaatatgtGttatatatttattaaggagttatccaacga gtgatagtttacgtGgttagatttgcatgttcaaatctaaaataagcaattacaCtatagtgagagaaagttagagagag acgct
