Omixon HLA Twin™ (Software)

Agenda   § 

Introduc)on  to  

 (15  mins)  

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Hands  on  workflows  in  HLA  Twin  (45  mins)   §  Per-­‐sample  indexing  with  Holotype  HLA  X4  data   §  High  Quality  7-­‐locus  Holotype  HLA  X2  data   §  Novel  alleles  with  IMGT/HLA  v3.10   §  Export  consensus  

§  Novel  alleles  with  IMGT/HLA  v3.19   § 

Ques)ons  -­‐  just  put  your  hand  up  –  we’ll  be  right  over!  

Omixon  HLA  Twin™  (So4ware)   § 

SoXware  component  of  Holotype  HLA  

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Automated  genotyping  aXer  MiSeq  run  

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Preconfigured  Holotype  HLA-­‐specific  se\ngs  

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Two  algorithms  for  determining  HLA  genotypes   –  Consensus  Genotyping  (Assembly)     –  Sta)s)cal  Genotyping  (Alignment  to  IMGT/HLA)  

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Traffic  light  system  for  data  interpreta)on  and  workflow   management   PASSED   WARNING   FAILED  

The  Traffic  Light  System  

Implementa@on  HLA  Twin  

Summary  cards  

View  budon  

Sample  Details  

Browse  Alignment  

Naviga)on  budons  

3  Hands-­‐on  Workflows  in  Twin     § 

Per-­‐sample  indexing  with  Holotype  HLA  X4  data  

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High  Quality  7-­‐locus  Holotype  HLA  X2  data  

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Novel  Allele  Discovery   –  Discover  novel  allele  with  IMGT/HLA  3.10   §  Export  consensus  

–  Resolve  novel  allele  by  reanalyzing  with  IMGT/HLA  3.19