Proteome analysis of Bacillus subtilis extracellular ... - Semantic Scholar

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Microbiology (2000), 146, 65–75

Printed in Great Britain

Proteome analysis of Bacillus subtilis extracellular proteins : a two-dimensional protein electrophoretic study Isao Hirose, Kazuyoshi Sano, Izumi Shioda, Miyuki Kumano, Kouji Nakamura and Kunio Yamane Author for correspondence : Kunio Yamane. Tel : ­81 298 53 6680. Fax : ­81 298 53 6680. e-mail : kyamane!sakura.cc.tsukuba.ac.jp

Institute of Biological Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki 305, Japan

To analyse the proteome of Bacillus subtilis extracellular proteins, extracellular protein samples were prepared from culture media (minimal medium containing 0