Recreational and Surface Water Scheme - Gov.uk

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Summary of Results External Quality Assessment of Water Microbiology

Recreational and Surface Water Scheme Distribution Number: S81

Sample Numbers: S81A, S81B

Distribution Date: Results Due: Report Date:

November 2017 08 December 2017 21 December 2017

Samples prepared and quality control tested by:

Angela Appea Richard Borrill Thomas Harper Zak Prior Judith Spellar Aneta Stranc Lili Tsegaye

Data analysed by:

Joanna Donn Nita Patel

Report compiled by:

Joanna Donn Nita Patel

Authorised by:

Nita Patel

This report must not be reproduced without permission of the organisers. Public Health England Food and Environmental Proficiency Testing Unit (FEPTU) 61 Colindale Avenue London NW9 5EQ Tel: +44 (0)20 8327 7119 Fax: +44 (0)20 8200 8264 Email: [email protected] The data in FEPTU reports is confidential

Page 1 of 18

Printed at 10:57 on Thursday, 21 December, 2017

For further information on the scheme please refer to: Scheme Guide: https://www.gov.uk/government/publications/food-and-water-proficiency-testing-schemes-scheme-guide Guide to Scoring and Statistics: https://www.gov.uk/government/publications/food-and-water-proficiency-testing-schemes-scoring-systems-and-statistics

General guidance for z-scores: Participants' enumeration results are converted into z-scores using the following formula:

Z = (xi-Xpt) σpt

xi Xpt

= participants' result expressed (expressed as a log 10 value) = assigned value (participants' consensus median (expressed as a log 10 value)

σpt

= the fixed standard deviation for the examination (calculated by FEPTU)

The σpt-value expresses the acceptable difference between the individual participant 's result and the participants' consensus median. The σpt-value used for calculating z-scores for all parameters in the Recreational and Surface Water Scheme is 0.35. A guide to interpreting z-scores follows, although laboratories must interpret their scores in the context of their own laboratory situation. z = -1.99 to +1.99 z = -2 to -2.99 or +2 to +2.99 z= < -3.00 or > + 3.00

satisfactory questionable unsatisfactory

It is usually recommended that z-scores exceeding +/-2 are investigated to establish the possible cause. general rule, PHE recommends that all questionable and unsatisfactory results are investigated.

As a

FEPTU Quality Control: To demonstrate homogeneity of the sample, a minimum of 10 LENTICULE® discs , selected randomly from a batch, are tested in duplicate for parameters requiring enumeration.

To demonstrate stability of the sample, a minimum of nine LENTICULE discs, selected randomly from a batch , are examined throughout the distribution period for enumeration parameters. PHE uses methods stipulated in the Microbiology of Recreational and Environmental Waters (2000) and the DWI’s series of documents: The Microbiology of Drinking Water (2002) - Methods for the Examination of Waters and Associated Materials. The FEPTU results are used for guidance in the preliminary intended results notification, letters are posted on the website immediately after every distribution; electronic notification of their availability is sent to all participants. Refer to section 17.0 of the Scheme Guide if you have experienced difficulties with any of the examinations . https://www.gov.uk/government/publications/food-and-water-proficiency-testing-schemes-scheme-guide

Participants are reminded that reporting an incorrect or false negative results for water samples could have serious public health implications. Participants who report unexpectedly high or low results for enumerations on single occasions only should not be unduly alarmed, although they should still assess the reason(s) for the outlying result.

Please contact FEPTU staff for advice and information: Repeat samples

Carmen Gomes or Kermin Daruwalla

Data Analysis

Manchari Rajkumar or Nita Patel

Microbiological advice

Nita Patel or Zak Prior

General comments and complaints

Nita Patel or Zak Prior

Scheme consultants

Julie E. Russell

Scheme Co-ordinator

Nita Patel

Tel: +44 (0)20 8327 7119 Fax: +44 (0)20 8200 8264 Email: [email protected] FEPTU's website

Accreditation: PHE Water EQA Scheme for Recreational and Surface Water is accredited by the United Kingdom Accreditation Service (UKAS) to ISO/IEC 17043:2010.

0006 The data in FEPTU reports is confidential

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Printed at 10:57 on Thursday, 21 December, 2017

Sample: S81A Water sample from river, lake and stream

Contents:

Enterobacter cloacae (wild strain), Enterococcus faecium (wild strain), Clostridium perfringens (wild strain), Salmonella Derby 1,4,[5],12:f,g:[1,2] (wild strain), Providencia rettgeri (NCTC 7475)

Expected Results: All counts are expressed as colony forming units (cfu) per 100mL. The fixed standard deviation value (σpt value) used for calculation of the z-scores is 0.35 for all parameters.

Parameter

FEPTU median

Coliform bacteria

Escherichia coli

Enterococci

Faecal coliforms

63

0

59

0

Clostridium perfringens

Salmonella spp.

75

Detected in

(1.88 log₁₀)

1L

No. results returned

68

77

71

34

58

45

Assigned value

72

0

63

0

87

N/A

(Participants

(1.94 log₁₀)

median all results) Uncertainty of

0.02

N/A

0.01

N/A

0.03

N/A

71

0

63

0

83

N/A

assigned value* Participants mean (all results)

(1.92 log₁₀)

Expected Range

23 - 228

N/A

20 - 199

N/A

28 - 2.75x10²

N/A

0.14

N/A

0.09

N/A

0.15

N/A

1

N/A

2

N/A

3

N/A

False positives

N/A

2

N/A

6

N/A

N/A

False negatives

1

N/A

1

N/A

1

0

Standard deviation** No of outlying counts

Your result

Score for performance assessment Z-score

*

U(Xpt) is based on results transformed to a log₁₀ scale

**

Robust S* based on median absolute deviation about the participants' median (MADe)

Total sent samples

85

Not examined

4

Non returns

2

Late returns

0

The data in FEPTU reports is confidential

Page 3 of 18

Printed at 10:57 on Thursday, 21 December, 2017

Sample: S81B Water sample from river, lake and stream

Contents:

Escherichia coli (wild strain), Klebsiella pneumoniae (wild strain), Enterococcus faecalis (wild strain), Clostridium perfringens (wild strain), Salmonella Stanley 1,4,[5],12,[27]:d:1,2 (wild strain)

Expected Results: All counts are expressed as colony forming units (cfu) per 100mL. The fixed standard deviation value (σpt value) used for calculation of the z-scores is 0.35 for all parameters.

Parameter

FEPTU median

Coliform bacteria

Escherichia coli

Enterococci

Faecal coliforms

Clostridium perfringens

Salmonella spp.

1.07x10²

93

81

1.01x10²

2.90x10²

Detected in

(2.03 log₁₀)

(1.97 log₁₀)

(1.91 log₁₀)

(2 log₁₀)

(2.46 log₁₀)

1L

68

77

71

35

58

45

1.65x10²

40

91

81

2.85x10²

N/A

(2.22 log₁₀)

(1.6 log₁₀)

(1.96 log₁₀)

(1.91 log₁₀)

(2.45 log₁₀)

0.03

0.1

0.01

0.1

0.04

N/A

1.58x10²

94

90

69

2.68x10²

N/A

(2.2 log₁₀)

(1.97 log₁₀)

(1.95 log₁₀)

(1.84 log₁₀)

(2.43 log₁₀)

52 - 5.22x10²

10 - 1.50x10²

29 - 2.88x10²

12 - 2.56x10²

90 - 9.00x10²

N/A

0.2

0.28

0.08

0.43

0.21

N/A

5

38

0

5

4

N/A

False positives

N/A

N/A

N/A

N/A

N/A

N/A

False negatives

0

35

0

0

1

2

No. results returned

Assigned value (Participants median all results) Uncertainty of assigned value* Participants mean (all results) Expected Range

Standard deviation** No of outlying counts

Your result

Score for performance assessment Z-score

*

U(Xpt) is based on results transformed to a log₁₀ scale

**

Robust S* based on median absolute deviation about the participants' median (MADe)

Total sent samples

85

Not examined

3

Non returns

2

Late returns

0

The data in FEPTU reports is confidential

Page 4 of 18

Printed at 10:57 on Thursday, 21 December, 2017

S81A: Coliform bacteria

25

Outlying results

72 Participant Median 63 FEPTU Median

Outlying results

30

No. of Results

20

15

10

>228

207-228

196-206

185-195

174-184

163-173

152-162

141-151

130-140

119-129

97-107

86-96

75-85

64-74

54-63

44-53

34-43

23-33

228

207-228

196-206

185-195

174-184

163-173

152-162

141-151

130-140

119-129

108-118

97-107

86-96

75-85

64-74

54-63

44-53

34-43

23-33

0

199

180-199

170-179

160-169

150-159

140-149

130-139

120-129

110-119

92-100

83-91

74-82

65-73

56-64

47-55

38-46

29-37

20-28

199

180-199

170-179

160-169

150-159

140-149

130-139

120-129

110-119

101-109

92-100

83-91

74-82

65-73

56-64

47-55

38-46

29-37

20-28

0

2.44

2.37-2.44

2.32-2.36

2.28-2.31

2.24-2.27

2.20-2.23

2.16-2.19

2.12-2.15

2.08-2.11

2.03-2.07

1.99-2.02

1.95-1.98

1.91-1.94

1.87-1.90

1.83-1.86

1.78-1.82

1.74-1.77

1.70-1.73

1.66-1.69

1.62-1.65

1.58-1.61

2.44

2.37-2.44

2.32-2.36

2.28-2.31

2.24-2.27

2.20-2.23

2.16-2.19

2.12-2.15

2.08-2.11

2.03-2.07

1.99-2.02

1.95-1.98

1.91-1.94

1.87-1.90

1.83-1.86

1.78-1.82

1.74-1.77

1.70-1.73

1.66-1.69

1.62-1.65

1.58-1.61

1.53-1.57

1.49-1.52

1.45-1.48

0

2.72

2.64-2.72

2.60-2.63

2.55-2.59

2.51-2.54

2.47-2.50

2.42-2.46

2.38-2.41

2.34-2.37

2.29-2.33

2.25-2.28

2.21-2.24

2.16-2.20

2.12-2.15

2.08-2.11

2.03-2.07

1.99-2.02

1.95-1.98

1.90-1.94

1.86-1.89

1.82-1.85

1.77-1.81

2.72

2.64-2.72

2.60-2.63

2.55-2.59

2.51-2.54

2.47-2.50

2.42-2.46

2.38-2.41

2.34-2.37

2.29-2.33

2.25-2.28

2.21-2.24

2.16-2.20

2.12-2.15

2.08-2.11

2.03-2.07

1.99-2.02

1.95-1.98

1.90-1.94

1.86-1.89

1.82-1.85

1.77-1.81

1.73-1.76

1.60-1.72

0

2.30

2.18-2.30

2.08-2.18

2.03-2.07

1.98-2.02

1.93-1.97

1.88-1.92

1.83-1.87

1.78-1.82

1.73-1.77

1.68-1.72

1.62-1.67

1.57-1.61

1.52-1.56

1.47-1.51

1.42-1.46

1.37-1.41

1.32-1.36

1.27-1.31

1.22-1.26

1.17-1.21

1.11-1.16

2.30

40

1.60 Participant Median 1.97 FEPTU Median

Outlying results

45

Outlying results

50

2.18-2.30

S81B: Escherichia coli

30 25 20 15 10 5

2.08-2.18

2.03-2.07

1.98-2.02

1.93-1.97

1.88-1.92

1.83-1.87

1.78-1.82

1.73-1.77

1.68-1.72

1.62-1.67

1.57-1.61

1.52-1.56

1.47-1.51

1.42-1.46

1.37-1.41

1.32-1.36

1.27-1.31

1.22-1.26

1.17-1.21

1.11-1.16

1.06-1.10

1.01-1.05

0

2.46

2.39-2.46

2.34-2.38

2.30-2.33

2.26-2.29

2.22-2.25

2.18-2.21

2.14-2.17

2.09-2.13

2.05-2.08

2.01-2.04

1.97-2.00

1.93-1.96

1.89-1.92

1.85-1.88

1.80-1.84

1.76-1.79

1.72-1.75

1.68-1.71

1.64-1.67

1.60-1.63

1.55-1.59

2.46

2.39-2.46

2.34-2.38

2.30-2.33

2.26-2.29

2.22-2.25

2.18-2.21

2.14-2.17

2.09-2.13

2.05-2.08

2.01-2.04

1.97-2.00

1.93-1.96

1.89-1.92

1.85-1.88

1.80-1.84

1.76-1.79

1.72-1.75

1.68-1.71

1.64-1.67

1.60-1.63

1.55-1.59

1.51-1.54

1.47-1.50

0

2.41

2.30-2.41

2.25-2.29

2.19-2.24

2.13-2.18

2.07-2.12

2.01-2.06

1.96-2.00

1.90-1.95

1.84-1.89

1.78-1.83

1.73-1.77

1.67-1.72

1.61-1.66

1.55-1.60

1.50-1.54

1.44-1.49

1.38-1.43

1.32-1.37

1.26-1.31

1.21-1.25

1.15-1.20

2.41

2.30-2.41

2.25-2.29

2.19-2.24

2.13-2.18

2.07-2.12

2.01-2.06

1.96-2.00

1.90-1.95

1.84-1.89

1.78-1.83

1.73-1.77

1.67-1.72

1.61-1.66

1.55-1.60

1.50-1.54

1.44-1.49

1.38-1.43

1.32-1.37

1.26-1.31

1.21-1.25

1.15-1.20

1.09-1.14

0.89-1.08

0

2.95

2.88-2.95

2.83-2.87

2.79-2.82

2.75-2.78

2.70-2.74

2.66-2.69

2.62-2.65

2.57-2.61

2.53-2.56

2.49-2.52

2.44-2.48

2.40-2.43

2.36-2.39

2.31-2.35

2.27-2.30

2.23-2.26

2.18-2.22

2.14-2.17

2.10-2.13

2.05-2.09

2.95

2.88-2.95

2.83-2.87

2.79-2.82

2.75-2.78

2.70-2.74

2.66-2.69

2.62-2.65

2.57-2.61

2.53-2.56

2.49-2.52

2.44-2.48

2.40-2.43

2.36-2.39

2.31-2.35

2.27-2.30

2.23-2.26

2.18-2.22

2.14-2.17

2.10-2.13

2.05-2.09

2.01-2.04

1.96-2.00

1.86-1.95

0