S1 SUPPORTING INFORMATION Title: Photothermal therapy of ...
SUPPORTING INFORMATION Title: Photothermal therapy of glioblastoma multiforme using multiwalled carbon nanotubes optimized for diffusion in extracellular space Authors: Brittany N. Eldridge1‡, Brian W. Bernish1‡, Cale D. Fahrenholtz1, Ravi Singh1,2* 1 Department of Cancer Biology, Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, NC, 27157, USA; 2 Comprehensive Cancer Center of Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA * Ravi Singh, Ph.D., Assistant Professor, Department of Cancer Biology, Wake Forest School of Medicine, Hanes Bldg., Rm. 4045, Medical Center Blvd., Winston-Salem, NC 27157; Phone: 336-713-4434; FAX: 336-716-0255; Email: [email protected]
ASSOCIATED CONTENT: 5 pages; Supplemental figures S1-S4
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Supplemental Figure S1. Evaluation of the cytotoxicity induced by MWCNTs modified by acid oxidation and coating with various surfactants. A) U87, U373, and D54 GBM cells or NHA cells were treated for 48 h with increasing doses of acid oxidized MWCNTs dispersed in the indicated surfactants. After 48 h, cells were lysed, pelleted and the supernatant was analyzed for ATP content as a measure of cell viability using the CellTiter-Glo assay. Samples were prepared and measured in sextuplicate and are displayed as the mean ± standard deviation of each measurement. Significant differences in viability between cells treated with 1% or 2% DSPE-PEG coated MWCNTs and uncoated or Pluronic F-127 coated MWCNTs (determined by ANOVA followed by Student’s T-Test when appropriate) are indicated by (*; p
ASSOCIATED CONTENT: 5 pages; Supplemental figures S1-S4
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Supplemental Figure S1. Evaluation of the cytotoxicity induced by MWCNTs modified by acid oxidation and coating with various surfactants. A) U87, U373, and D54 GBM cells or NHA cells were treated for 48 h with increasing doses of acid oxidized MWCNTs dispersed in the indicated surfactants. After 48 h, cells were lysed, pelleted and the supernatant was analyzed for ATP content as a measure of cell viability using the CellTiter-Glo assay. Samples were prepared and measured in sextuplicate and are displayed as the mean ± standard deviation of each measurement. Significant differences in viability between cells treated with 1% or 2% DSPE-PEG coated MWCNTs and uncoated or Pluronic F-127 coated MWCNTs (determined by ANOVA followed by Student’s T-Test when appropriate) are indicated by (*; p
