Supp Fig 1
A
B
Number of Cells
15
Undiff
10
5
Day 6 49
47
46
45
44
40
0
Number of Chromosomes
48hrs post Nucleofection, 5µg, 1x106 Phase GFP Phase GFP
D-33 K-33
NPC
C-33
X-01
C-31
X-33
G-33
A-28 A-29
AN-33
B-33
A-31
Z-33
B-30
A-32
Y-33
B-29
C-33
40hrs post Nucleofection, 2µg, 1.5x106 Phase GFP Phase GFP
A-27
C
MECP2 ATRX XIST
A-33
NPC
D
24hrs post Nucleofection, 2x106
24hrs post Nucleofection, D33, 5µg plasmid, 2x106 No PCI
E33
With PCI
No DNA control
10µg plasmid 5µg plasmid
D33
E
F
Endotoxin-containing
Endotoxin-free
Endotoxin-free, RPMI, Spin
Endotoxin-free, RPMI
B
Number of Cells
15
Undiff
10
5
Day 6 49
47
46
45
44
40
0
Number of Chromosomes
48hrs post Nucleofection, 5µg, 1x106 Phase GFP Phase GFP
D-33 K-33
NPC
C-33
X-01
C-31
X-33
G-33
A-28 A-29
AN-33
B-33
A-31
Z-33
B-30
A-32
Y-33
B-29
C-33
40hrs post Nucleofection, 2µg, 1.5x106 Phase GFP Phase GFP
A-27
C
MECP2 ATRX XIST
A-33
NPC
D
24hrs post Nucleofection, 2x106
24hrs post Nucleofection, D33, 5µg plasmid, 2x106 No PCI
E33
With PCI
No DNA control
10µg plasmid 5µg plasmid
D33
E
F
Endotoxin-containing
Endotoxin-free
Endotoxin-free, RPMI, Spin
Endotoxin-free, RPMI
