Table S1. Oligonucleotide primers for newly ... - Semantic Scholar
Table S1. Oligonucleotide primers for newly developed molecular markers for fine-mapping sed1. Marker name umc1917
X8
G1
X18
X22
X224
X93
umc2390
X41
X52
bnlg1811
umc1770
Order
Primer Sequence
F (5’–3’)
ACTTCCACTTCACCAGCCTTTTC
R(5’–3’)
GGAAAGAAGAGCCGCTTGGT
F (5’–3’)
ACCTTCGACAACAAAACCCCC
R(5’–3’)
GATTGGATGCGTGCGCCTGT
F (5’–3’)
AGGCAAATGGCATGTATC
R(5’–3’)
TGGCACTGGTAGGGTTAA
F (5’–3’)
CGGTGAAGGCATCAGAAGGG
R(5’–3’)
GGGAGGACGGCAAGCAAGAG
F (5’–3’)
TTCTGCCGTTGGTCATTCAT
R(5’–3’)
TTGGAGGTCATTGCACAAGA
F (5’–3’)
ATCGCATCACAAGTCACAGCC
R(5’–3’)
TGCCAATCATTCACTAAGCCAC
F (5’–3’)
ACACGAGGGTTAGCCAGTTA
R(5’–3’)
GACCGAGTGTCATAAAGAAGG
F (5’–3’)
GAAATGGCAGGGAAACTTGTTTAT
R(5’–3’)
AAGAGGCAAGCAAGTGTACAGTGA
F (5’–3’)
TTGTTGCTGCTGTGGTTGTT
R(5’–3’)
CATCCCTCGTTTCCTTAGCG
F (5’–3’)
AATAACCCTGCTGCGTGCTG
R(5’–3’)
TTTGGATGATCTGGGACTGG
F (5’–3’)
ACACAAGCCGACCAAAAAAC
R(5’–3’)
GTAGTAGGAACGGGCGATGA
F (5’–3’)
GAGGGATCATGGCTCTCTTCC
R(5’–3’)
GTCCATCATCAGCCTGTCACC
Table S2. Progeny testing of T-HIR for UH400 plants by two normal inbred lines EmA kernel
HIR
No.
(%)
51
40
6.36
967
73
39
7.55
U-1-1
279
15
16
5.38
U-1-2
1209
71
40
5.87
C7-2
U-1-3
138
10
8
7.25
2010
C7-2
U-1-4
404
36
21
8.91
2010
C7-2
U-1-5
371
21
14
5.66
2010
C7-2
U-1-6
336
24
15
7.14
2010
C7-2
U-1-7
541
37
28
6.84
2010
C7-2
U-1-8
107
6
7
5.61
2010
C7-2
U-1-9
379
20
31
5.28
2010
C7-2
U-1-10
142
8
12
5.63
2010
C7-2
U-1-11
175
10
10
2010
C7-2
2010
Z58
U-1-1
308
27
18
8.77
2010
Z58
U-1-3
465
32
20
6.88
2010
Z58
U-1-4
402
28
16
6.97
2010
Z58
U-1-7
136
13
5
9.56
2010
Z58
U-1-8
101
8
7
7.92
2010
Z58
U-1-9
392
24
28
6.12
2010
Z58
U-1-11
515
41
25
7.96
2010
Z58
Male parent
Normal kernel
ID
No.
C7-2
U-1
802
2009
Z58
U-1
2010
C7-2
2010
C7-2
2010
Year
Female parent
2009
Haploid
Mean
Mean
5.71 6.30
7.74
Note: U-1 represented one UH400 plant in 2009 in Beijing; U-1-1 to U-1-11 represented the progeny of U-1 planted in 2010 in Beijing.
Figure S1
Figure S1. Schematic diagram for the pollen competitive ability (PCA) test procedure for lines 1680 and UH400. Two kinds of pollen were pollinated on one plant in different time intervals. M1= Male 1 parent; M2= Male 2. ●= pollinations performed.
Figure S2
Figure S2. Punnett square for genetic transmission of the sed1 haplotype through male and female gametes in F2 populations with different survive rates ri for the different genotypes.
Figure S3
Figure S3. Ear phenotype in reciprocal crossing and selfing ears. A: ear phenotypes of crosses, when inducers were used as female parents and normal lines as male parents, showed rare defective kernels and good seed set rate; B: self-pollinated ear phenotypes of different inducers; C: ear phenotypes of crosses, when normal lines were used as female parents and inducers as male parents, showed many defective kernels and pool seed set rate.
X8
G1
X18
X22
X224
X93
umc2390
X41
X52
bnlg1811
umc1770
Order
Primer Sequence
F (5’–3’)
ACTTCCACTTCACCAGCCTTTTC
R(5’–3’)
GGAAAGAAGAGCCGCTTGGT
F (5’–3’)
ACCTTCGACAACAAAACCCCC
R(5’–3’)
GATTGGATGCGTGCGCCTGT
F (5’–3’)
AGGCAAATGGCATGTATC
R(5’–3’)
TGGCACTGGTAGGGTTAA
F (5’–3’)
CGGTGAAGGCATCAGAAGGG
R(5’–3’)
GGGAGGACGGCAAGCAAGAG
F (5’–3’)
TTCTGCCGTTGGTCATTCAT
R(5’–3’)
TTGGAGGTCATTGCACAAGA
F (5’–3’)
ATCGCATCACAAGTCACAGCC
R(5’–3’)
TGCCAATCATTCACTAAGCCAC
F (5’–3’)
ACACGAGGGTTAGCCAGTTA
R(5’–3’)
GACCGAGTGTCATAAAGAAGG
F (5’–3’)
GAAATGGCAGGGAAACTTGTTTAT
R(5’–3’)
AAGAGGCAAGCAAGTGTACAGTGA
F (5’–3’)
TTGTTGCTGCTGTGGTTGTT
R(5’–3’)
CATCCCTCGTTTCCTTAGCG
F (5’–3’)
AATAACCCTGCTGCGTGCTG
R(5’–3’)
TTTGGATGATCTGGGACTGG
F (5’–3’)
ACACAAGCCGACCAAAAAAC
R(5’–3’)
GTAGTAGGAACGGGCGATGA
F (5’–3’)
GAGGGATCATGGCTCTCTTCC
R(5’–3’)
GTCCATCATCAGCCTGTCACC
Table S2. Progeny testing of T-HIR for UH400 plants by two normal inbred lines EmA kernel
HIR
No.
(%)
51
40
6.36
967
73
39
7.55
U-1-1
279
15
16
5.38
U-1-2
1209
71
40
5.87
C7-2
U-1-3
138
10
8
7.25
2010
C7-2
U-1-4
404
36
21
8.91
2010
C7-2
U-1-5
371
21
14
5.66
2010
C7-2
U-1-6
336
24
15
7.14
2010
C7-2
U-1-7
541
37
28
6.84
2010
C7-2
U-1-8
107
6
7
5.61
2010
C7-2
U-1-9
379
20
31
5.28
2010
C7-2
U-1-10
142
8
12
5.63
2010
C7-2
U-1-11
175
10
10
2010
C7-2
2010
Z58
U-1-1
308
27
18
8.77
2010
Z58
U-1-3
465
32
20
6.88
2010
Z58
U-1-4
402
28
16
6.97
2010
Z58
U-1-7
136
13
5
9.56
2010
Z58
U-1-8
101
8
7
7.92
2010
Z58
U-1-9
392
24
28
6.12
2010
Z58
U-1-11
515
41
25
7.96
2010
Z58
Male parent
Normal kernel
ID
No.
C7-2
U-1
802
2009
Z58
U-1
2010
C7-2
2010
C7-2
2010
Year
Female parent
2009
Haploid
Mean
Mean
5.71 6.30
7.74
Note: U-1 represented one UH400 plant in 2009 in Beijing; U-1-1 to U-1-11 represented the progeny of U-1 planted in 2010 in Beijing.
Figure S1
Figure S1. Schematic diagram for the pollen competitive ability (PCA) test procedure for lines 1680 and UH400. Two kinds of pollen were pollinated on one plant in different time intervals. M1= Male 1 parent; M2= Male 2. ●= pollinations performed.
Figure S2
Figure S2. Punnett square for genetic transmission of the sed1 haplotype through male and female gametes in F2 populations with different survive rates ri for the different genotypes.
Figure S3
Figure S3. Ear phenotype in reciprocal crossing and selfing ears. A: ear phenotypes of crosses, when inducers were used as female parents and normal lines as male parents, showed rare defective kernels and good seed set rate; B: self-pollinated ear phenotypes of different inducers; C: ear phenotypes of crosses, when normal lines were used as female parents and inducers as male parents, showed many defective kernels and pool seed set rate.
