Table S1. Primers used in this study. Primer Name Sequence ... - PLOS
Table S1. Primers used in this study. Primer Name
Sequence (5’-‐3’)a
Primers for SAg expression constructs NcoI-‐His-‐TEV-‐SpeA For BamHI-‐SpeA Rev SpeA-‐Y100A For NcoI-‐His-‐TEV-‐SpeG For BamHI-‐SpeG Rev NcoI-‐SpeL-‐Forward BamHI-‐SpeL-‐Reverse NcoI-‐SpeM-‐Forward BamHI-‐SpeM-‐Reverse SmeZ-‐TEV-‐KpnI For SmeZ-‐BamHI Rev
CCCCCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCGAAAACTTGTATTTCCAAGGCCAACAAGACCCCGATCCAAGCCA CCCGGATCCTTACTTGGTTGTTAGGTAGACTTCAAT AGTGCATGTATCGCCGGAGGGGTAACA CCCCCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCGAAAACTTGTATTTCCAAGGCGATGAAAATTTAAAAGATTTAAAA CCCGGATCCCTAGTACGTTTTTAAGTAGATATCAAA GGGCCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCGAAAACTTGTATTTCCAAAGTGAAGAGACTATTAATATTAAGGAT GGGGGATCCTTATTTATTAAATTTAACTAAGTA GGGCCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCGAAAACTTGTATTTCCAAAGTGATGCTGTGTTGGTTAATAGCGAA GGGGGATCCCTAATTTTTAGAAAAATCTTCGTT CCCGGTACCGGTGGTGGCTCCGGTGAAAACTTGTATTTCCAAGGCTTAGAAGTAGATAATAATTCC CCCGGATCCTTAGGAGTCAATTTCTATATC
Primers for SAg gene chromosomal deletion constructs BamHI-‐upstream speA MGAS8232 For PstI-‐upstream speA MGAS8232 Rev PstI-‐downstream speA MGAS8232 For KpnI-‐downstream speA MGAS8232 Rev PstI-‐downstream speC MGAS8232 For KpnI-‐downstream speC MGAS8232Rev BamHI-‐upstream speC MGAS8232 For PstI-‐upstream speC MGAS8232 Rev BamHI-‐upstream speG MGAS8232 For PstI-‐upstream speG MGAS8232 Rev PstI-‐downstream speG MGAS8232 For KpnI-‐downstream speG MGAS8232 Rev BamHI-‐upstream speL/M MGAS8232 For PstI-‐upstream speL/M MGAS8232 Rev PstI-‐downstream speL/M MGAS8232 For KpnI-‐downstream speL/M MGAS8232 Rev BamHI-‐upstream smeZ MGAS8232 For PstI-‐upstream smeZ MGAS8232 Rev PstI-‐downstream smeZ MGAS8232 For KpnI-‐downstream smeZ MGAS8232 Rev
CCCGGATCCGCCAAGTTATGCCATTACTGTGTTG CCCCTGCAGGAAGTCTACCTAACAACCAAGTAA CCCCTGCAGCAATACTTTTTTATTGTTTTCCATTAATAT CCCGGTACCGCAAATGACAAATCGCTATATCAATAA CCCCTGCAGTTTGATGATGTTAATCTTTTTCAT CCCGGTACCCGGATATCAATTTTGTGGATTAAACG CCCGGATCCCGGTTACACCATGCGCACTGTCG CCCCTGCAGTTCGATATTTATCTTGAAAAATAATTCATC CCCGGATCCGCCCAGCTTCAAGACGTTGGTTAA CCCCTGCAGAAATTCTAAAATGAACTTAGCCAC CCCCTGCAGGATATCTACTTAAAAACGCACTAG CCCGGTACCGGAAGATCAAGCCAACCCAAGAAA CCCGGATCCCCTATGGACGTAGACAAATATGTTG CCCCTGCAGTTATTCACAAACAAAGAAAATTAATTAGTA CCCCTGCAGCAAAGTCAAGGTATTTTTTTTCAT CGCGGTACCCGGTGTATTGACATTGATGTATTC GGGGGATCCGGGGAATTATGCCAATTGTCTCTA CCCCTGCAGAAAAATAAGTTTTGTTTTTTTCATAAATAG CCCCTGCAGTTAGATATAGAAATTGACTCCTAATTC GGGGGTACCGGGCAATTGTTTAACTGGTTAATTAG
Primers for the SpeA and SpeAY100A complementation constructs KpnI-‐tsf For EcoRI-‐MCS-‐tsf Rev EcoRI-‐MCS-‐pepO For NotI-‐pepO Rev SpeI-‐speA Comp For SalI-‐speA Comp Rev
GGGGGGGGTACCACTTGCTCAATTGAACCACG TTTTTTGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCTCCGTTTGACACAACAAAAAGA GGGGGGGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGACACCAATAAGGAAGCAAAAA GGGGGGGCGGCCGCAGCCTAAATGATTGGTGGA GGGGGGACTAGTATTTTATAATAAAATTATTAATATAAGTTAA GGGGGGGTCGACAAAACCGCTCATCAAATGA
Screening and sequencing primers for genomic integrations Int/Screen speA For speA Screen II 8232 Rev speC Int II For speC Int II Rev speG int II For speG Screen II Rev speL/M Int II For speL/M Screen II Rev smeZ Int II For smeZ Screen II Rev
GCAAGCTACGGTTACGAAATTG CCATCCTTTTTTGATTCTCC GCAAAGCACTGGTTCGATGT GGATAACCTTAACCGCGCTAC GCCCTCGTCAGAATGACTGT CCGATACCGATCACCACAAG GGCTTGAGTTATGTGTCTTTA GTTTGGTGGTATAGATGTAGCAAGG CATGCCTGCTCAAACAAGATT ATACGACTCCATCTCATTATAGC
Superantigen gene internal screening primers. speA RT For speA RT Rev speC RT For speC RT Rev speG RT For speG RT Rev speL RT For speL RT Rev speM RT For speM RT Rev smeZ RT For smeZ RT Rev
AAAGTTGCCATCTCTTGGTTC CAAGAGGTATTTGCTCAACAAGAC TTTGAGCAGGCGTAATTCCT TTCAACGACACACACATTAAACA ACCCCATGCGATTATGAAAA GGGAGACCAAAAACATCGAC ATAAGTCAGCACCTTCCTCTTTC AAATCTCCCGTTACCTTCCA AACTTCTTCTTCCTTAAAGCGTCT TGCTGTGTTGGTTAATAGCGA TTTCTCGTCCTGTGATTGGA AATGGGACGGAGAACATAGC
Mouse genotyping primers DQA (1027) For DQA (1029) Rev DQ8b (vA) For1 DQ8b (vT) For 2
GAAGACATTGTGGCTGACCATGTTGCC AGCACAGCGATGTTTGTCAGTGCAAATTGCGG AGGATTTGGTGTACCAGTTTAAGGGCAT AGGATTTGGTGTTCCAGTTTAAGGGCAT
DQ8b (vii) Rev DR4A (1101) For DR4A (1104) Rev DR4B (1098) For DR4B (1099) Rev a
TGCAAGGTCGTGCGGAGCTCCAA GGAGATAGTGGAACTTGCGG CCGATCACCAATGTACCTCC GTTTCTTGGAGCAGGTTAAACA CTGCACTGTGAAGCTCTCAC
Underlined sequences in primers indicate restriction endonuclease sites used for cloning purposes.
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Sequence (5’-‐3’)a
Primers for SAg expression constructs NcoI-‐His-‐TEV-‐SpeA For BamHI-‐SpeA Rev SpeA-‐Y100A For NcoI-‐His-‐TEV-‐SpeG For BamHI-‐SpeG Rev NcoI-‐SpeL-‐Forward BamHI-‐SpeL-‐Reverse NcoI-‐SpeM-‐Forward BamHI-‐SpeM-‐Reverse SmeZ-‐TEV-‐KpnI For SmeZ-‐BamHI Rev
CCCCCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCGAAAACTTGTATTTCCAAGGCCAACAAGACCCCGATCCAAGCCA CCCGGATCCTTACTTGGTTGTTAGGTAGACTTCAAT AGTGCATGTATCGCCGGAGGGGTAACA CCCCCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCGAAAACTTGTATTTCCAAGGCGATGAAAATTTAAAAGATTTAAAA CCCGGATCCCTAGTACGTTTTTAAGTAGATATCAAA GGGCCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCGAAAACTTGTATTTCCAAAGTGAAGAGACTATTAATATTAAGGAT GGGGGATCCTTATTTATTAAATTTAACTAAGTA GGGCCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCGAAAACTTGTATTTCCAAAGTGATGCTGTGTTGGTTAATAGCGAA GGGGGATCCCTAATTTTTAGAAAAATCTTCGTT CCCGGTACCGGTGGTGGCTCCGGTGAAAACTTGTATTTCCAAGGCTTAGAAGTAGATAATAATTCC CCCGGATCCTTAGGAGTCAATTTCTATATC
Primers for SAg gene chromosomal deletion constructs BamHI-‐upstream speA MGAS8232 For PstI-‐upstream speA MGAS8232 Rev PstI-‐downstream speA MGAS8232 For KpnI-‐downstream speA MGAS8232 Rev PstI-‐downstream speC MGAS8232 For KpnI-‐downstream speC MGAS8232Rev BamHI-‐upstream speC MGAS8232 For PstI-‐upstream speC MGAS8232 Rev BamHI-‐upstream speG MGAS8232 For PstI-‐upstream speG MGAS8232 Rev PstI-‐downstream speG MGAS8232 For KpnI-‐downstream speG MGAS8232 Rev BamHI-‐upstream speL/M MGAS8232 For PstI-‐upstream speL/M MGAS8232 Rev PstI-‐downstream speL/M MGAS8232 For KpnI-‐downstream speL/M MGAS8232 Rev BamHI-‐upstream smeZ MGAS8232 For PstI-‐upstream smeZ MGAS8232 Rev PstI-‐downstream smeZ MGAS8232 For KpnI-‐downstream smeZ MGAS8232 Rev
CCCGGATCCGCCAAGTTATGCCATTACTGTGTTG CCCCTGCAGGAAGTCTACCTAACAACCAAGTAA CCCCTGCAGCAATACTTTTTTATTGTTTTCCATTAATAT CCCGGTACCGCAAATGACAAATCGCTATATCAATAA CCCCTGCAGTTTGATGATGTTAATCTTTTTCAT CCCGGTACCCGGATATCAATTTTGTGGATTAAACG CCCGGATCCCGGTTACACCATGCGCACTGTCG CCCCTGCAGTTCGATATTTATCTTGAAAAATAATTCATC CCCGGATCCGCCCAGCTTCAAGACGTTGGTTAA CCCCTGCAGAAATTCTAAAATGAACTTAGCCAC CCCCTGCAGGATATCTACTTAAAAACGCACTAG CCCGGTACCGGAAGATCAAGCCAACCCAAGAAA CCCGGATCCCCTATGGACGTAGACAAATATGTTG CCCCTGCAGTTATTCACAAACAAAGAAAATTAATTAGTA CCCCTGCAGCAAAGTCAAGGTATTTTTTTTCAT CGCGGTACCCGGTGTATTGACATTGATGTATTC GGGGGATCCGGGGAATTATGCCAATTGTCTCTA CCCCTGCAGAAAAATAAGTTTTGTTTTTTTCATAAATAG CCCCTGCAGTTAGATATAGAAATTGACTCCTAATTC GGGGGTACCGGGCAATTGTTTAACTGGTTAATTAG
Primers for the SpeA and SpeAY100A complementation constructs KpnI-‐tsf For EcoRI-‐MCS-‐tsf Rev EcoRI-‐MCS-‐pepO For NotI-‐pepO Rev SpeI-‐speA Comp For SalI-‐speA Comp Rev
GGGGGGGGTACCACTTGCTCAATTGAACCACG TTTTTTGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCTCCGTTTGACACAACAAAAAGA GGGGGGGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGACACCAATAAGGAAGCAAAAA GGGGGGGCGGCCGCAGCCTAAATGATTGGTGGA GGGGGGACTAGTATTTTATAATAAAATTATTAATATAAGTTAA GGGGGGGTCGACAAAACCGCTCATCAAATGA
Screening and sequencing primers for genomic integrations Int/Screen speA For speA Screen II 8232 Rev speC Int II For speC Int II Rev speG int II For speG Screen II Rev speL/M Int II For speL/M Screen II Rev smeZ Int II For smeZ Screen II Rev
GCAAGCTACGGTTACGAAATTG CCATCCTTTTTTGATTCTCC GCAAAGCACTGGTTCGATGT GGATAACCTTAACCGCGCTAC GCCCTCGTCAGAATGACTGT CCGATACCGATCACCACAAG GGCTTGAGTTATGTGTCTTTA GTTTGGTGGTATAGATGTAGCAAGG CATGCCTGCTCAAACAAGATT ATACGACTCCATCTCATTATAGC
Superantigen gene internal screening primers. speA RT For speA RT Rev speC RT For speC RT Rev speG RT For speG RT Rev speL RT For speL RT Rev speM RT For speM RT Rev smeZ RT For smeZ RT Rev
AAAGTTGCCATCTCTTGGTTC CAAGAGGTATTTGCTCAACAAGAC TTTGAGCAGGCGTAATTCCT TTCAACGACACACACATTAAACA ACCCCATGCGATTATGAAAA GGGAGACCAAAAACATCGAC ATAAGTCAGCACCTTCCTCTTTC AAATCTCCCGTTACCTTCCA AACTTCTTCTTCCTTAAAGCGTCT TGCTGTGTTGGTTAATAGCGA TTTCTCGTCCTGTGATTGGA AATGGGACGGAGAACATAGC
Mouse genotyping primers DQA (1027) For DQA (1029) Rev DQ8b (vA) For1 DQ8b (vT) For 2
GAAGACATTGTGGCTGACCATGTTGCC AGCACAGCGATGTTTGTCAGTGCAAATTGCGG AGGATTTGGTGTACCAGTTTAAGGGCAT AGGATTTGGTGTTCCAGTTTAAGGGCAT
DQ8b (vii) Rev DR4A (1101) For DR4A (1104) Rev DR4B (1098) For DR4B (1099) Rev a
TGCAAGGTCGTGCGGAGCTCCAA GGAGATAGTGGAACTTGCGG CCGATCACCAATGTACCTCC GTTTCTTGGAGCAGGTTAAACA CTGCACTGTGAAGCTCTCAC
Underlined sequences in primers indicate restriction endonuclease sites used for cloning purposes.
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