This study highlights the presence of MRSA and PVL toxin among ...
Detection of Membrane Damaging Toxin Determinants among Staphylococcus aureus causing Skin and Soft Tissue Infections Damodharan
1 Perumal ,
Nagaraj
1 Perumal ,
Stalin
2 Mani ,
Betsy Sowndarya
1 Dass ,
Padma
1 Krishnan*
1. Department of Microbiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai. 2. Department of Microbiology, Government Dharmapuri Medical College, Dharmapuri.
INTRODUCTION Staphylococcus aureus is a well armed pathogen with an array of virulence factors. Staphylococcus involves huge array of membrane damaging toxins.(i.e)Alpha (Hla), Beta (Hlb), Gamma (Hlg), Delta (Hld), Panton – Valentine Leukocidin - LukS. These toxins are associated with the high virulence potential among community acquired skin and soft tissue infections. AIM & OBJECTIVES
Results Among the 50 isolates, 14 (28%) were found to be MRSA and 10 (20%) were MSSA. Among these isolates, 24/38(63%) were PVL-MRSA and 2/12 isolates (16.7%) were PVL-MSSA. 42/50 isolates (84%) were found to be positive for alpha-hemolysin (hla) and all the 50 isolates were positive for delta-hemolysin(hld ) None of the isolates was positive for beta (hlb) and gamma hemolysin (hlg).
28%
The study was designed to detect the prevalence of membrane damaging toxins among S.aureus isolates causing skin and soft tissue infection. MATERIALS AND METHODS Total 50 isolates were included in the study, All the isolates were associated with skin and soft tissue infection. DNA was extracted by boiling lysis method. All the isolates were subjected to PCR.
Molecular detection of membrane damaging toxin genes by Multiplex PCR MULTIPLEX PCR – In house method Nagarajan et al., (2013) Hemolysin gene detection – Jarruad et al., (2000)
PVL – MRSA & MSSA
MRSA AND MSSA
20%
30
50
25
40
20
14 10
15 10
Hlb
24
100%
84%
50
20
42
10
2
0 PVL-MRSA
Conclusion:
HEMOLYSIN GENES
30
5 Hla
60
PVL-MSSA
0
0
Hla
Hlb
0
Hld
This study highlights the presence of MRSA and PVL toxin among community isolates of S. aureus causing skin and soft tissue infection.
Hlg
1 Perumal ,
Nagaraj
1 Perumal ,
Stalin
2 Mani ,
Betsy Sowndarya
1 Dass ,
Padma
1 Krishnan*
1. Department of Microbiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai. 2. Department of Microbiology, Government Dharmapuri Medical College, Dharmapuri.
INTRODUCTION Staphylococcus aureus is a well armed pathogen with an array of virulence factors. Staphylococcus involves huge array of membrane damaging toxins.(i.e)Alpha (Hla), Beta (Hlb), Gamma (Hlg), Delta (Hld), Panton – Valentine Leukocidin - LukS. These toxins are associated with the high virulence potential among community acquired skin and soft tissue infections. AIM & OBJECTIVES
Results Among the 50 isolates, 14 (28%) were found to be MRSA and 10 (20%) were MSSA. Among these isolates, 24/38(63%) were PVL-MRSA and 2/12 isolates (16.7%) were PVL-MSSA. 42/50 isolates (84%) were found to be positive for alpha-hemolysin (hla) and all the 50 isolates were positive for delta-hemolysin(hld ) None of the isolates was positive for beta (hlb) and gamma hemolysin (hlg).
28%
The study was designed to detect the prevalence of membrane damaging toxins among S.aureus isolates causing skin and soft tissue infection. MATERIALS AND METHODS Total 50 isolates were included in the study, All the isolates were associated with skin and soft tissue infection. DNA was extracted by boiling lysis method. All the isolates were subjected to PCR.
Molecular detection of membrane damaging toxin genes by Multiplex PCR MULTIPLEX PCR – In house method Nagarajan et al., (2013) Hemolysin gene detection – Jarruad et al., (2000)
PVL – MRSA & MSSA
MRSA AND MSSA
20%
30
50
25
40
20
14 10
15 10
Hlb
24
100%
84%
50
20
42
10
2
0 PVL-MRSA
Conclusion:
HEMOLYSIN GENES
30
5 Hla
60
PVL-MSSA
0
0
Hla
Hlb
0
Hld
This study highlights the presence of MRSA and PVL toxin among community isolates of S. aureus causing skin and soft tissue infection.
Hlg
