Boric acid Buffer grade A0768 Literature
Page 1 of 2
Boric acid Buffer grade
Solubility (20°C)
50 g/L (H2O)
Melting point
185°C (dec.)
Formula
BH3O3
M
61.83 g/mol
CAS-No.:
10043-35-3
HS-No.:
28100090
EC-No.:
233-139-2
Storage:
RT
LGK:
6.1 D
Disposal:
28
R:
60-61
S:
53-45 toxic for reproduction
WGK:
1
Specification
Assay (titr.)
min. 99.5 %
pH (5 %; H2O; 20°C)
3.5 - 4.8
Heavy metals (as Pb)
max. 0.001 %
Water
max. 0.5 %
Chloride
max. 0.005 %
Sulfate
max. 0.02 %
Literature (1) Peacock, A.C. & Dingman, C.W. (1968) Biochemistry 7, 668-674 Molecular weight estimation and separation of ribonucleic acid by electrophoresis in Agarose-Acrylamide composite gels. (2) Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87 Electrophoresis in agarose and acrylamide gels.
A0768
Page 2 of 2
Boric acid Buffer grade
A0768
Comment The best and most commonly used running buffer for non-denaturing or denaturing polyacrylamide gels is a Tris-Borate-EDTA buffer (TBE) with a pH of 8.3. Usually the buffer is prepared as a 10X concentrate (Tris base 890 mM, Boric acid 890 mM and EDTA disodium salt dihydrate 250 mM)
Boric acid Buffer grade
Solubility (20°C)
50 g/L (H2O)
Melting point
185°C (dec.)
Formula
BH3O3
M
61.83 g/mol
CAS-No.:
10043-35-3
HS-No.:
28100090
EC-No.:
233-139-2
Storage:
RT
LGK:
6.1 D
Disposal:
28
R:
60-61
S:
53-45 toxic for reproduction
WGK:
1
Specification
Assay (titr.)
min. 99.5 %
pH (5 %; H2O; 20°C)
3.5 - 4.8
Heavy metals (as Pb)
max. 0.001 %
Water
max. 0.5 %
Chloride
max. 0.005 %
Sulfate
max. 0.02 %
Literature (1) Peacock, A.C. & Dingman, C.W. (1968) Biochemistry 7, 668-674 Molecular weight estimation and separation of ribonucleic acid by electrophoresis in Agarose-Acrylamide composite gels. (2) Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87 Electrophoresis in agarose and acrylamide gels.
A0768
Page 2 of 2
Boric acid Buffer grade
A0768
Comment The best and most commonly used running buffer for non-denaturing or denaturing polyacrylamide gels is a Tris-Borate-EDTA buffer (TBE) with a pH of 8.3. Usually the buffer is prepared as a 10X concentrate (Tris base 890 mM, Boric acid 890 mM and EDTA disodium salt dihydrate 250 mM)
