Cardona Lorgeoux Goguenheim
EFFECT OF BIOFLOC REARING SYSTEMS ON REPRODUCTIVE PERFORMANCES AND QUALITY OF LARVAE OF THE SHRIMP Litopenaeus stylirostris
Emilie CARDONA (1) (2), Bénédicte LORGEOUX(1), Liet CHIM(2), Jean GOGUENHEIM (1) (1) IFREMER,
Tahiti Oceanology center of Pacific ; Laboratory Marine ressources
(2) IFREMER,
New Caledonia ; Laboratory Lagoon Ecosystem and Durable Aquaculture 1
Introduction
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Experimentations carried out in 2 french territories located in Pacific region : New Caledonia & French Polynesia (Tahiti)
2
Objective
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To compare reproductive performances and quality of larvae of L stylirostris according to the rearing systems :
Extensive Earthen ponds Intensive Clear Water Intensive Biofloc
3
2 Trials
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First trial (New Caledonia): Comparison traditionnal earthen ponds methods vs biofloc technology Second trial (Tahiti) : Comparison clear water vs biofloc Parameters monitored : • •
Measured parameters on broodstock Health statuts : antioxidant defenses Reproductive performances parameters : spawn number, egg number Eggs and larval quality : antioxidant defenses, larval survival rate
4
2 Trials
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Several steps: Grow-out the shrimps to reproduction size Transfer in hatchery : - Separation of female/male according the differents treatments - All animals with same conditions (nutrition. temperature. salinity…) Eyestalk ablation Reproduction - Maturation - Artificial insemination - Spawning - Eggs and nauplii - Zoe 2 and Post larvae 1 (just for the 2nd experimentation) 5
lfremer First trial (New Caledonia): Comparison traditionnal earthen ponds methods vs biofloc technology
6
Biofloc vs Earthen pond
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Rearing duration: 4 months Biofloc (BFT): 4 tanks of 4m² C/N=12/1 Density : 20 shrimps/m² Daily 3% of water renewal
Extensive earthen ponds (EEP): 4 ponds Density : 0.5 shrimps/m2 Daily up to 30% of water renewal
7
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Grow out results Parameters Survival rate (%) Growth rate (g.day-1) Finale biomass (g.m-3)
EEP 64.1 ± 11.5 0.17 ± 0.01 13.6 ± 1.5
BFT 74.7 ± 8.2 0.13 ± 0.01 600.1 ± 97.2
S NS * ***
8
Antioxydant defenses statut EEP
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BFT MDA 3 2
Carbonyl protein
CAT 1 0
GSH T
SOD T
GPx
Star plot representation of oxidative status and antioxidant defenses in the digestive gland of broodstock after growth out period
9
Reproductive performances
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Total spawning number. eggs and nauplii produced based on one hundred females per treatment (EEP vs BFT) EEP
BFT
Total spawning number 84 ± 25.4 122 ± 16.2 Total eggs number 12.73 107 ± 3.85 107 21.11 107± 2.82 107 Total nauplii number 7.79 107 ± 2.35 107 14.79 107± 1.98 107
Gain
S
43% 63% 87%
* * **
10
Cumulative spawning - %
Reproductive performances
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160
EEP
140
BFT
120
Day 0 : eyestalk
100 80 60 40 20 0 0
5
10
Times - day
11
Cumulative spawning - %
Reproductive performances
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160
EEP
140
BFT
120
Day 0 : eyestalk
100 80 60 40 20 0 0
5
10
Times - day 90
Rank 1
Cumulative spawning -%
80 70 60 50 40
30 20 10 0 0
5 Times - day 10
12
Cumulative spawning - %
Reproductive performances
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160
EEP
140
BFT
120
Day 0 : eyestalk
100 80 60 40 20 0 0
5
10
Times - day 90
Rank 1
Cumulative spawning -%
80
Rank 2
70 60
35%
50 40
30
17%
20 10 0 0
5 Times - day 10
0
5
Times - day
10
13
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Cumulative spawning - %
Reproductive performances 160
EEP
140
BFT
120
Day 0 : eyestalk
100 80 60 40 20 0 0
5
10
Times - day 90
Rank 1
Cumulative spawning -%
80
Rank 2
Rank 3
70 60 50 40
16%
30 20
2%
10 0 0
5 Times - day 10
0
5
Times - day
10
0
5
Times - day
14 10
Glutathion
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BFT shrimps exhibited significantly higher glutathion (gsh) in ovaries before spawn BFT : 14.41 ± 1.85 EEP : 5.17 ± 1.66 Unity : µmol.mg protein-1
15
Glutathion
BFT shrimps exhibited significantly higher glutathion (gsh) in ovaries before spawn
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Glutathion : powerful antioxydant peptide
BFT : 14.41 ± 1.85 EEP : 5.17 ± 1.66 Unity : µmol.mg protein-1
16
Glutathion
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Biofloc particles : high gsh concentration 2.37± 1.45mM.g-1of protein BFT shrimps exhibited significantly higher glutathion (gsh) in ovaries before spawn BFT : 14.41 ± 1.85 EEP : 5.17 ± 1.66 Unity : µmol.mg protein-1
17
Glutathion
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Biofloc particles : high gsh concentration 2.37± 1.45mM.g-1of protein BFT shrimps exhibited significantly higher glutathion (gsh) in ovaries before spawn BFT : 14.41 ± 1.85 EEP : 5.17 ± 1.66 Unity : µmol.mg protein-1
GSH found in eggs from BFT fem : Eggs from BFT fem: 19.15±1.57 Eggs from EPP fem 12.64±2.25 Unity :µmol.mg protein-1 18
lfremer Second trial (Tahiti) : Comparison clear water vs biofloc
19
Biofloc vs Clear water
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Rearing duration : 2.5 months Biofloc (BFT) 3 tanks of 25m²: C/N=20/1 Density : 12 shrimps/m² Daily 3% of water renewal
Clear water (CW) : 3 tanks of 25m² Density : 12 shrimps/m² Daily 200% of water renewal
20
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Grow out results Zootechnical results after 2.5months of rearing
Parameters
CW
BFT
Individual female weight (g) 48.25 ± 5.25 48.93 ± 4.13 Survival rate (%)
49.12 ± 13.98 53.72 ± 7.29
S NS NS
21
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Antioxidant status Antioxidant in female broodstock (digestive gland)
Parameters Tot. antioxidant status (µmol.mg of proteine-1) Glutathion (µmol.mg of proteine-1)
CW
BFT
S
53.66 ± 10.48
65.61 ± 6.54
*
1.20 ± 0.49
2.56 ± 1.04
*
22
Reproductive performance
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60
BFT
50
CW
50%
Higher spawn number for BFT females
40 30 20
30%
10 0 0
1
2
3
4 5 6 7 8 Tiime (days)
Higher eggs number for BFT females
9 10 Cumulative eggs number spawn x106
Cumulative spawning - %
70
140 120 100 80 60 40 20 0
BFT CW
0
1
2
3
Twice more eggs
4 5 6 7 Time (day)
8
9 10 23
Reproductive performance
IGS (%)
8
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Gonado somatic index
7
Significant Higher gonado somatic index
6 5 4 CW BFT Broodstock origin
24
Reproductive performance
IGS (%)
8
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Gonado somatic index
7
Significant Higher gonado somatic index
6 5 4 CW BFT Broodstock origin
Parameters CW BFT S eggs number/ spawn 175726 ± 52876 202325 ± 60118 * Higher eggs number per spawn for BFT females
25
Larval survival
Parameters CW BFT Zoe 2 stage survival (%) 70.06 ± 13.79 87.98 ± 23.8 PL1 stage survival (%) 45.48 ± 7.67 68.73 ± 12.60
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S NS *
26
Results summary and hypothesis
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Results : Better antioxydant status of broodstock in BFT Higher reproductive performance & larvae quality Hypothesis: Bioactive molecules which could modulate the reproduction of the female broodstock Bioactive molecules maybe transfer into eggs (as we show for glutathion) which lead to a better health of larvae
27
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Thank you for your attention
28
Emilie CARDONA (1) (2), Bénédicte LORGEOUX(1), Liet CHIM(2), Jean GOGUENHEIM (1) (1) IFREMER,
Tahiti Oceanology center of Pacific ; Laboratory Marine ressources
(2) IFREMER,
New Caledonia ; Laboratory Lagoon Ecosystem and Durable Aquaculture 1
Introduction
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Experimentations carried out in 2 french territories located in Pacific region : New Caledonia & French Polynesia (Tahiti)
2
Objective
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To compare reproductive performances and quality of larvae of L stylirostris according to the rearing systems :
Extensive Earthen ponds Intensive Clear Water Intensive Biofloc
3
2 Trials
lfremer
First trial (New Caledonia): Comparison traditionnal earthen ponds methods vs biofloc technology Second trial (Tahiti) : Comparison clear water vs biofloc Parameters monitored : • •
Measured parameters on broodstock Health statuts : antioxidant defenses Reproductive performances parameters : spawn number, egg number Eggs and larval quality : antioxidant defenses, larval survival rate
4
2 Trials
lfremer
Several steps: Grow-out the shrimps to reproduction size Transfer in hatchery : - Separation of female/male according the differents treatments - All animals with same conditions (nutrition. temperature. salinity…) Eyestalk ablation Reproduction - Maturation - Artificial insemination - Spawning - Eggs and nauplii - Zoe 2 and Post larvae 1 (just for the 2nd experimentation) 5
lfremer First trial (New Caledonia): Comparison traditionnal earthen ponds methods vs biofloc technology
6
Biofloc vs Earthen pond
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Rearing duration: 4 months Biofloc (BFT): 4 tanks of 4m² C/N=12/1 Density : 20 shrimps/m² Daily 3% of water renewal
Extensive earthen ponds (EEP): 4 ponds Density : 0.5 shrimps/m2 Daily up to 30% of water renewal
7
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Grow out results Parameters Survival rate (%) Growth rate (g.day-1) Finale biomass (g.m-3)
EEP 64.1 ± 11.5 0.17 ± 0.01 13.6 ± 1.5
BFT 74.7 ± 8.2 0.13 ± 0.01 600.1 ± 97.2
S NS * ***
8
Antioxydant defenses statut EEP
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BFT MDA 3 2
Carbonyl protein
CAT 1 0
GSH T
SOD T
GPx
Star plot representation of oxidative status and antioxidant defenses in the digestive gland of broodstock after growth out period
9
Reproductive performances
lfremer
Total spawning number. eggs and nauplii produced based on one hundred females per treatment (EEP vs BFT) EEP
BFT
Total spawning number 84 ± 25.4 122 ± 16.2 Total eggs number 12.73 107 ± 3.85 107 21.11 107± 2.82 107 Total nauplii number 7.79 107 ± 2.35 107 14.79 107± 1.98 107
Gain
S
43% 63% 87%
* * **
10
Cumulative spawning - %
Reproductive performances
lfremer
160
EEP
140
BFT
120
Day 0 : eyestalk
100 80 60 40 20 0 0
5
10
Times - day
11
Cumulative spawning - %
Reproductive performances
lfremer
160
EEP
140
BFT
120
Day 0 : eyestalk
100 80 60 40 20 0 0
5
10
Times - day 90
Rank 1
Cumulative spawning -%
80 70 60 50 40
30 20 10 0 0
5 Times - day 10
12
Cumulative spawning - %
Reproductive performances
lfremer
160
EEP
140
BFT
120
Day 0 : eyestalk
100 80 60 40 20 0 0
5
10
Times - day 90
Rank 1
Cumulative spawning -%
80
Rank 2
70 60
35%
50 40
30
17%
20 10 0 0
5 Times - day 10
0
5
Times - day
10
13
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Cumulative spawning - %
Reproductive performances 160
EEP
140
BFT
120
Day 0 : eyestalk
100 80 60 40 20 0 0
5
10
Times - day 90
Rank 1
Cumulative spawning -%
80
Rank 2
Rank 3
70 60 50 40
16%
30 20
2%
10 0 0
5 Times - day 10
0
5
Times - day
10
0
5
Times - day
14 10
Glutathion
lfremer
BFT shrimps exhibited significantly higher glutathion (gsh) in ovaries before spawn BFT : 14.41 ± 1.85 EEP : 5.17 ± 1.66 Unity : µmol.mg protein-1
15
Glutathion
BFT shrimps exhibited significantly higher glutathion (gsh) in ovaries before spawn
lfremer
Glutathion : powerful antioxydant peptide
BFT : 14.41 ± 1.85 EEP : 5.17 ± 1.66 Unity : µmol.mg protein-1
16
Glutathion
lfremer
Biofloc particles : high gsh concentration 2.37± 1.45mM.g-1of protein BFT shrimps exhibited significantly higher glutathion (gsh) in ovaries before spawn BFT : 14.41 ± 1.85 EEP : 5.17 ± 1.66 Unity : µmol.mg protein-1
17
Glutathion
lfremer
Biofloc particles : high gsh concentration 2.37± 1.45mM.g-1of protein BFT shrimps exhibited significantly higher glutathion (gsh) in ovaries before spawn BFT : 14.41 ± 1.85 EEP : 5.17 ± 1.66 Unity : µmol.mg protein-1
GSH found in eggs from BFT fem : Eggs from BFT fem: 19.15±1.57 Eggs from EPP fem 12.64±2.25 Unity :µmol.mg protein-1 18
lfremer Second trial (Tahiti) : Comparison clear water vs biofloc
19
Biofloc vs Clear water
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Rearing duration : 2.5 months Biofloc (BFT) 3 tanks of 25m²: C/N=20/1 Density : 12 shrimps/m² Daily 3% of water renewal
Clear water (CW) : 3 tanks of 25m² Density : 12 shrimps/m² Daily 200% of water renewal
20
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Grow out results Zootechnical results after 2.5months of rearing
Parameters
CW
BFT
Individual female weight (g) 48.25 ± 5.25 48.93 ± 4.13 Survival rate (%)
49.12 ± 13.98 53.72 ± 7.29
S NS NS
21
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Antioxidant status Antioxidant in female broodstock (digestive gland)
Parameters Tot. antioxidant status (µmol.mg of proteine-1) Glutathion (µmol.mg of proteine-1)
CW
BFT
S
53.66 ± 10.48
65.61 ± 6.54
*
1.20 ± 0.49
2.56 ± 1.04
*
22
Reproductive performance
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60
BFT
50
CW
50%
Higher spawn number for BFT females
40 30 20
30%
10 0 0
1
2
3
4 5 6 7 8 Tiime (days)
Higher eggs number for BFT females
9 10 Cumulative eggs number spawn x106
Cumulative spawning - %
70
140 120 100 80 60 40 20 0
BFT CW
0
1
2
3
Twice more eggs
4 5 6 7 Time (day)
8
9 10 23
Reproductive performance
IGS (%)
8
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Gonado somatic index
7
Significant Higher gonado somatic index
6 5 4 CW BFT Broodstock origin
24
Reproductive performance
IGS (%)
8
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Gonado somatic index
7
Significant Higher gonado somatic index
6 5 4 CW BFT Broodstock origin
Parameters CW BFT S eggs number/ spawn 175726 ± 52876 202325 ± 60118 * Higher eggs number per spawn for BFT females
25
Larval survival
Parameters CW BFT Zoe 2 stage survival (%) 70.06 ± 13.79 87.98 ± 23.8 PL1 stage survival (%) 45.48 ± 7.67 68.73 ± 12.60
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S NS *
26
Results summary and hypothesis
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Results : Better antioxydant status of broodstock in BFT Higher reproductive performance & larvae quality Hypothesis: Bioactive molecules which could modulate the reproduction of the female broodstock Bioactive molecules maybe transfer into eggs (as we show for glutathion) which lead to a better health of larvae
27
lfremer
Thank you for your attention
28
