M2 macrophages eliminate renal crystals by phagocytosis Kazumi Taguchi, Atsushi Okada, Takahiro Yasui, Rei Unno, Kazuhiro Niimi, Masayuki Usami, Shuzo Hamamoto, Masahito Hirose, Ryosuke Ando, Keiichi Tozawa, Yutaro Hayashi, and Kenjiro Kohri Department of Nephro-urology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Study 2 Immunohistohchemistry : x20 (inset:x400)
op/op
+/+
+/+
M1Mφ
op/op
Fluorescent immunohistochemistry
Pizzolato
150
(day6)
+/+
op/op
CSF-1 op/op
op/op
CSF-1 op/op
100
Pan-Mφ
50 0
x40 0
M2Mφ
350 300
Renal crystal deposit (%)
*
*
250
*
*
1.5
†
Green:iNOS
†§
†
†
M2Mφ
†§
+/+
200
†
1.0
Exposure of labbed-calcium oxalate monohydrate (COM) crystals to each cell culture (0h, 1h, 6h, 24h) (Chaiyarit S. et al. J Proteome Res. 2012)