D C A B E F

A

B

GSAPsi MedGC APPsi

GSAPsi MedGC APPsi

MedGC

GSAP si

APP si

120

Nicastrin

APP

98 kDa

37 kDa

GAPDH

37

GAPDH

kDa

C

D

Flourescence intensity

6000

MedGC

GSAPsi

5000 4000

MEDGC

3000

GSAP KD

2000

Kif11 KD

1000 0

0

5

10

15

20

25

Replicates

E

F

350

mRNA levels of GSAP relative to the control, MEDGC

300

LDH (mU/ml)

250 200 150 100 50 0

MEDGC secreted MEDGC intracellular GSAP si secreted GSAP si intracellular LDH LDH LDH LDH

G

1.4 1.2 1.0 0.8 0.6 0.4

***

0.2 0.0

MEDGC

GSAP si

n.s

Alamar Blue Values (Cell Viability)

5000 4000 3000 2000 1000 0

MEDGC-DMSO

MEDGC-Imatinib

Scrambled oligos

GSAP-DMSO

GSAP-Imatinib

siRNA specific against GSAP

Sup Fig. 1