Jang Kim
No Discharge
High Production
High Food Safety
High Immunity
Low FCR
No antibiotics
High Biosecurity
Low CO2 emmission
Initial B.W. (g)
Stocking Density (/m2)
Days
Final B.W. (g)
Yield (kg/m2)
Total Harvest (kg)
Survival rate(%)
FCR
Mean
0.13
465.5
145
16.7
4.83
2,754
64.5
1.91
Min
0.038
404
112
13.6
3.37
2,020
33.7
1.19
Max
0.26
500
177
19.9
6.40
3,280
88.3
2.91
W.T. DO (℃) (mg/L)
Sal
pH
TAN NO2-N NO3-N Chl-a TSS VSS (mg/L) (mg/L) (mg/L) (㎍/L) (mg/L) (mg/L)
Mean 28.5
5.6
32.9
7.3
0.45
3.35
Min 28.1
5.1
31.4
7.0
0.2
Max 29.2
6.2
34.3
7.7
0.8
Alkal (mg/L)
Tur. (NTU)
57.7 206.9 821.4
312.2
157.6
152.8
1.2
33.2
565
204.5
99
90.5
5.9
94.4 402.6 1134
383
216.3
212.2
52.2
WT (℃)
DO (mg/L)
Sal (psu)
pH
TAN (mg/L)
NO2 (mg/L)
NO3 (mg/L)
TSS (mg/L)
VSS (mg/L)
Alk (mg/L)
Tur (NTU)
phase 1 (2013.06.03 - 07.11) tank1
28.9
6.2
32.3
7.4
0.65
0.38
33.76
375.8
296.4
137.1
206.2
tank2
28.6
7.0
32.0
7.4
0.92
1.55
28.63
327.8
237.3
142.2
177.1
phase 2 (2013.07.11 - 08.22) tank1
29.6
5.9
30.2
7.2
0.18
0.13
99.50
457.67
343.17
143.0
308.3
tank2
29.4
6.0
29.7
7.2
0.16
0.14
92.50
350.33
258.00
145.8
243.5
phase 3 (2013.08.23 -10.07) tank1
28.8
6.2
31.3
7.3
0.28
0.16
147.69
638.00
404.00
143.4
328.0
tank2
28.8
6.4
31.1
7.3
0.29
0.16
154.23
610.33
392.33
145.4
287.0
Bioflocs consist of bacteria, fungi, algae, detritus and other suspended particles (Hargreaves 2006, Avnimelech, 2011) Bacterial cell wall consists of LPS, PG and β -1, 3-glucans, activating the non-specific immune system (Johansson & Söderhäll 1985)
Bioflocs can be consumed by cultured animals (Avnimelech et al. 1994; McIntosh, 2000) Bioflocs may contribute to enhance the shrimp immunity
mas
proPO PPAE
Select six genes which are involved to innate immune response of shrimp proPO1(prophenoloxidase 1), proPO2 (prophenoloxidase 2), PPAE
(prophenoloxidase activating enzyme), SP1 (serine protease), mas (masquerade-like serine proteinase), Ran (ras-related nuclear)
Culture shrimp in biofloc media Mysis to PL3, postlarvae (PL20) and adult (about 10g)
Measure mRNA expression of six genes using real-time RT PCR
Name
GenBank accession No. & Remarks
proPO1-RT- F
EF115296.1
proPO1-RT- R
EF115296.1
proPO1-RT- P
EF115296.1
proPO2-RT- F
EF565469.1
proPO2-RT- R
EF565469.1
proPO2-RT- P
EF565469.1
PPAE1-RT- F
Jang et al., 2011
PPAE1-RT- R
Jang et al., 2011
PPAE1-RT- P
Jang et al., 2011
SP1- RT- F
JX644456, present study
SP1-RT- R
JX644456, present study
SP1-RT- P
JX644456, present study
mas-RT- F
JX644451, present study
mas-RT- R
JX644451, present study
mas-RT- P
JX644451, present study
Ran-RT- F
JX644455, present study
Ran-RT- R
JX644455, present study
Ran-RT- P
JX644455, present study
β-actin-RT- F
Jang et al., 2011
β-actin-RT- R
Jang et al., 2011
β-actin-RT- P
Jang et al., 2011
Experimental groups (L. vannamei) •
BF100 (TSS 678 mg/L), BF 75 (TSS 508), BF 50 (TSS 340), BF 25 (TSS 170), BF0 (TSS 13)
•
In short-term culture (two weeks) of L. vannamei postlarvae (2.2 mg BW)
•
BF100 (TSS 678 mg/L), BF 75 (TSS 508), BF 50 (TSS 340), BF 25 (TSS 170), BF0 (TSS 13)
•
Gene expression level was normalized to β-actin. *: P
High Production
High Food Safety
High Immunity
Low FCR
No antibiotics
High Biosecurity
Low CO2 emmission
Initial B.W. (g)
Stocking Density (/m2)
Days
Final B.W. (g)
Yield (kg/m2)
Total Harvest (kg)
Survival rate(%)
FCR
Mean
0.13
465.5
145
16.7
4.83
2,754
64.5
1.91
Min
0.038
404
112
13.6
3.37
2,020
33.7
1.19
Max
0.26
500
177
19.9
6.40
3,280
88.3
2.91
W.T. DO (℃) (mg/L)
Sal
pH
TAN NO2-N NO3-N Chl-a TSS VSS (mg/L) (mg/L) (mg/L) (㎍/L) (mg/L) (mg/L)
Mean 28.5
5.6
32.9
7.3
0.45
3.35
Min 28.1
5.1
31.4
7.0
0.2
Max 29.2
6.2
34.3
7.7
0.8
Alkal (mg/L)
Tur. (NTU)
57.7 206.9 821.4
312.2
157.6
152.8
1.2
33.2
565
204.5
99
90.5
5.9
94.4 402.6 1134
383
216.3
212.2
52.2
WT (℃)
DO (mg/L)
Sal (psu)
pH
TAN (mg/L)
NO2 (mg/L)
NO3 (mg/L)
TSS (mg/L)
VSS (mg/L)
Alk (mg/L)
Tur (NTU)
phase 1 (2013.06.03 - 07.11) tank1
28.9
6.2
32.3
7.4
0.65
0.38
33.76
375.8
296.4
137.1
206.2
tank2
28.6
7.0
32.0
7.4
0.92
1.55
28.63
327.8
237.3
142.2
177.1
phase 2 (2013.07.11 - 08.22) tank1
29.6
5.9
30.2
7.2
0.18
0.13
99.50
457.67
343.17
143.0
308.3
tank2
29.4
6.0
29.7
7.2
0.16
0.14
92.50
350.33
258.00
145.8
243.5
phase 3 (2013.08.23 -10.07) tank1
28.8
6.2
31.3
7.3
0.28
0.16
147.69
638.00
404.00
143.4
328.0
tank2
28.8
6.4
31.1
7.3
0.29
0.16
154.23
610.33
392.33
145.4
287.0
Bioflocs consist of bacteria, fungi, algae, detritus and other suspended particles (Hargreaves 2006, Avnimelech, 2011) Bacterial cell wall consists of LPS, PG and β -1, 3-glucans, activating the non-specific immune system (Johansson & Söderhäll 1985)
Bioflocs can be consumed by cultured animals (Avnimelech et al. 1994; McIntosh, 2000) Bioflocs may contribute to enhance the shrimp immunity
mas
proPO PPAE
Select six genes which are involved to innate immune response of shrimp proPO1(prophenoloxidase 1), proPO2 (prophenoloxidase 2), PPAE
(prophenoloxidase activating enzyme), SP1 (serine protease), mas (masquerade-like serine proteinase), Ran (ras-related nuclear)
Culture shrimp in biofloc media Mysis to PL3, postlarvae (PL20) and adult (about 10g)
Measure mRNA expression of six genes using real-time RT PCR
Name
GenBank accession No. & Remarks
proPO1-RT- F
EF115296.1
proPO1-RT- R
EF115296.1
proPO1-RT- P
EF115296.1
proPO2-RT- F
EF565469.1
proPO2-RT- R
EF565469.1
proPO2-RT- P
EF565469.1
PPAE1-RT- F
Jang et al., 2011
PPAE1-RT- R
Jang et al., 2011
PPAE1-RT- P
Jang et al., 2011
SP1- RT- F
JX644456, present study
SP1-RT- R
JX644456, present study
SP1-RT- P
JX644456, present study
mas-RT- F
JX644451, present study
mas-RT- R
JX644451, present study
mas-RT- P
JX644451, present study
Ran-RT- F
JX644455, present study
Ran-RT- R
JX644455, present study
Ran-RT- P
JX644455, present study
β-actin-RT- F
Jang et al., 2011
β-actin-RT- R
Jang et al., 2011
β-actin-RT- P
Jang et al., 2011
Experimental groups (L. vannamei) •
BF100 (TSS 678 mg/L), BF 75 (TSS 508), BF 50 (TSS 340), BF 25 (TSS 170), BF0 (TSS 13)
•
In short-term culture (two weeks) of L. vannamei postlarvae (2.2 mg BW)
•
BF100 (TSS 678 mg/L), BF 75 (TSS 508), BF 50 (TSS 340), BF 25 (TSS 170), BF0 (TSS 13)
•
Gene expression level was normalized to β-actin. *: P
