Modified Silica Nanoparticles
Supporting Information
Viral Inhibition Mechanism Mediated by SurfaceModified Silica Nanoparticles
Juliana Martins de Souza e Silva,1,2 Talita D. M. Hanchuk,3,4 Murilo Izidoro Santos,1 Jörg Kobarg,3,4 Marcio C. Bajgelman,3 Mateus B. Cardoso1*
1
Brazilian Synchrotron Light Laboratory, 13083-970, Campinas, Brazil
2
Lehrstuhl für Biomedizinische Physik, Physik-Department & Institut für Medizintechnik,
Technische Universität München, 85748, Garching, Germany 3
Brazilian Biosciences National Laboratory, 13083-970, Campinas, Brazil
4
Faculty of Pharmaceutical Sciences and Institute of Biology/Department of Biochemistry and
Tissue Biology - University of Campinas, 13083-862, Campinas, Brazil
*[email protected]
S-1
Table S1. Zeta-potential measurements carried out in different solvents Sample
Zeta-Potential (mV) 40 mM KCl
PBS
DMEM
mSiO2-TEOS
-9.6 ± 1.2
-16.7 ± 1.5
-20.2 ± 1.5
mSiO2-APTES
+4.5 ± 0.2
-12.8 ± 0.2
-18.8 ± 2.0
mSiO2-GPTMS
-13.9 ± 0.6
-11.2 ± 0.6
-17.5 ± 1.4
mSiO2-TMPES
-12.5 ± 0.2
-20.5 ± 0.9
-22.8 ± 1.6
Figure S1. Cytotoxicity assays. (A) HEK293T cells were incubated with different concentrations of mSiO2 particles for 48 h and their metabolic response was determined by the MTS assay. (B) Nanoparticles cytotoxic effect (10-5 g mL-1) was measured by flow cytometry on red emitting HEK293T cells co-transfected with DsRed expression vector.
S-2
Figure S2. Fluorescence assay of cells treated with nanoparticles. HEK293TCD4 cells were incubated with the nanoparticles prepared in this work (10-5 g mL-1) for 48 h. Cells nuclei were stained with DAPI and appear fluorescent blue. These images were used as negative control and illustrate the cytotoxicity test in Fig. S1 B. Scale bars: 10 μm.
S-3
Figure S3. Fluorescence images of negative controls of HIV tests with HEK293TCD4 cells. Images represent cells treated with nanoparticles, non-treated with HIV used in the two assays performed with HIV (in Fig. 4). Scale bars: 10 μm.
S-4
Figure S4. Molecular lipophilicity potential (MLP) evaluation. The MLP is calculated from the atomic hydrophobicity contributions of the functional groups. Here, it is color encoded with hydrophobic parts in blue and green while hydrophilic parts in orange and red.
S-5
Viral Inhibition Mechanism Mediated by SurfaceModified Silica Nanoparticles
Juliana Martins de Souza e Silva,1,2 Talita D. M. Hanchuk,3,4 Murilo Izidoro Santos,1 Jörg Kobarg,3,4 Marcio C. Bajgelman,3 Mateus B. Cardoso1*
1
Brazilian Synchrotron Light Laboratory, 13083-970, Campinas, Brazil
2
Lehrstuhl für Biomedizinische Physik, Physik-Department & Institut für Medizintechnik,
Technische Universität München, 85748, Garching, Germany 3
Brazilian Biosciences National Laboratory, 13083-970, Campinas, Brazil
4
Faculty of Pharmaceutical Sciences and Institute of Biology/Department of Biochemistry and
Tissue Biology - University of Campinas, 13083-862, Campinas, Brazil
*[email protected]
S-1
Table S1. Zeta-potential measurements carried out in different solvents Sample
Zeta-Potential (mV) 40 mM KCl
PBS
DMEM
mSiO2-TEOS
-9.6 ± 1.2
-16.7 ± 1.5
-20.2 ± 1.5
mSiO2-APTES
+4.5 ± 0.2
-12.8 ± 0.2
-18.8 ± 2.0
mSiO2-GPTMS
-13.9 ± 0.6
-11.2 ± 0.6
-17.5 ± 1.4
mSiO2-TMPES
-12.5 ± 0.2
-20.5 ± 0.9
-22.8 ± 1.6
Figure S1. Cytotoxicity assays. (A) HEK293T cells were incubated with different concentrations of mSiO2 particles for 48 h and their metabolic response was determined by the MTS assay. (B) Nanoparticles cytotoxic effect (10-5 g mL-1) was measured by flow cytometry on red emitting HEK293T cells co-transfected with DsRed expression vector.
S-2
Figure S2. Fluorescence assay of cells treated with nanoparticles. HEK293TCD4 cells were incubated with the nanoparticles prepared in this work (10-5 g mL-1) for 48 h. Cells nuclei were stained with DAPI and appear fluorescent blue. These images were used as negative control and illustrate the cytotoxicity test in Fig. S1 B. Scale bars: 10 μm.
S-3
Figure S3. Fluorescence images of negative controls of HIV tests with HEK293TCD4 cells. Images represent cells treated with nanoparticles, non-treated with HIV used in the two assays performed with HIV (in Fig. 4). Scale bars: 10 μm.
S-4
Figure S4. Molecular lipophilicity potential (MLP) evaluation. The MLP is calculated from the atomic hydrophobicity contributions of the functional groups. Here, it is color encoded with hydrophobic parts in blue and green while hydrophilic parts in orange and red.
S-5
