SUPPORTING INFORMATION In vivo detection of mitochondrial ...
SUPPORTING INFORMATION
In vivo detection of mitochondrial dysfunction induced by clinical drugs and disease-associated genes using a novel dye ZMJ214 in zebrafish
Shota Sasagawa1#, Yuhei Nishimura1,2,3,4,5#, Junko Koiwa1, Tsuyoshi Nomoto6, Taichi Shintou6, Soichiro Murakami1, Mizuki Yuge1, Koki Kawaguchi1, Reiko Kawase1, Takeshi Miyazaki6, and Toshio Tanaka1,2,3,4,5*
1Department
of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharamacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan 2Mie University Medical Zebrafish Research Center, Tsu, Mie 514-8507, Japan 3Depertment of Systems Pharmacology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan 4Department of Omics Medicine, Mie University Industrial Technology Innovation Institute, Tsu, Mie 514-8507, Japan 5Department of Bioinformatics, Mie University Life Science Research Center, Tsu, Mie 514-8507, Japan 6Corporate R&D Headquarters, Canon Inc., Ohta-ku, Tokyo 146-8501, Japan #These authors contributed equally to this work *Corresponding author 2-174 Edobashi, Tsu, Mie 514-8507, Japan Tel: +81-59-231-5411, Fax: +81-59-232-1765, E-mail: [email protected]
600
O1 MI1 MI2 O2 IO4
400 200 0
0
20 40 60 80 Troglitazone (µM)
O1 MI1 MI2 O2 IO4
400 200 0
0
20 40 60 80 Troglitazone (µM)
Area of neuromast (µm2)
Area of neuromast (µm2)
600
E
O1 MI1 MI2 O2 IO4
400 200 0
0
20 40 60 80 Pioglitazone (µM)
600
O1 MI1 MI2 O2 IO4
400 200 0
0
20 40 60 80 Pioglitazone (µM)
F 800
O1 MI1 MI2 O2 IO4
600 400 200 0
0
3 6 9 Tolcapone (µM)
12
G Area of neuromast (µm2)
600
D
Area of neuromast (µm2)
Area of neuromast (µm2)
C
Area of neuromast (µm2)
B
800
O1 MI1 MI2 O2 IO4
600 400 200 0
0
3 6 9 Entacaone (µM)
12
H 800
O1 MI1 MI2 O2 IO4
600 400 200 0
0
3 6 9 Tolcapone (µM)
12
Area of neuromast (µm2)
Area of neuromast (µm2)
A
800
O1 MI1 MI2 O2 IO4
600 400 200 0
0
3 6 9 Entacaone (µM)
12
Supplemental Figure 1. The changes of fluorescent area of the five neuromasts stained with ZMJ214 or YoPro1 in response to troglitazone, pioglitazone, tolcapone or entacapone. The changes of fluorescent area of the five neuromasts (O1, MI1, MI2, O2, and IO4) stained with ZMJ214 (A, B, E and F) or YoPro1 (C, D, G or H) in response to troglitazone (A and C), pioglitazone (B and D), tolcapone (E and G) or entacapone (F and H) are shown.
In vivo detection of mitochondrial dysfunction induced by clinical drugs and disease-associated genes using a novel dye ZMJ214 in zebrafish
Shota Sasagawa1#, Yuhei Nishimura1,2,3,4,5#, Junko Koiwa1, Tsuyoshi Nomoto6, Taichi Shintou6, Soichiro Murakami1, Mizuki Yuge1, Koki Kawaguchi1, Reiko Kawase1, Takeshi Miyazaki6, and Toshio Tanaka1,2,3,4,5*
1Department
of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharamacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan 2Mie University Medical Zebrafish Research Center, Tsu, Mie 514-8507, Japan 3Depertment of Systems Pharmacology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan 4Department of Omics Medicine, Mie University Industrial Technology Innovation Institute, Tsu, Mie 514-8507, Japan 5Department of Bioinformatics, Mie University Life Science Research Center, Tsu, Mie 514-8507, Japan 6Corporate R&D Headquarters, Canon Inc., Ohta-ku, Tokyo 146-8501, Japan #These authors contributed equally to this work *Corresponding author 2-174 Edobashi, Tsu, Mie 514-8507, Japan Tel: +81-59-231-5411, Fax: +81-59-232-1765, E-mail: [email protected]
600
O1 MI1 MI2 O2 IO4
400 200 0
0
20 40 60 80 Troglitazone (µM)
O1 MI1 MI2 O2 IO4
400 200 0
0
20 40 60 80 Troglitazone (µM)
Area of neuromast (µm2)
Area of neuromast (µm2)
600
E
O1 MI1 MI2 O2 IO4
400 200 0
0
20 40 60 80 Pioglitazone (µM)
600
O1 MI1 MI2 O2 IO4
400 200 0
0
20 40 60 80 Pioglitazone (µM)
F 800
O1 MI1 MI2 O2 IO4
600 400 200 0
0
3 6 9 Tolcapone (µM)
12
G Area of neuromast (µm2)
600
D
Area of neuromast (µm2)
Area of neuromast (µm2)
C
Area of neuromast (µm2)
B
800
O1 MI1 MI2 O2 IO4
600 400 200 0
0
3 6 9 Entacaone (µM)
12
H 800
O1 MI1 MI2 O2 IO4
600 400 200 0
0
3 6 9 Tolcapone (µM)
12
Area of neuromast (µm2)
Area of neuromast (µm2)
A
800
O1 MI1 MI2 O2 IO4
600 400 200 0
0
3 6 9 Entacaone (µM)
12
Supplemental Figure 1. The changes of fluorescent area of the five neuromasts stained with ZMJ214 or YoPro1 in response to troglitazone, pioglitazone, tolcapone or entacapone. The changes of fluorescent area of the five neuromasts (O1, MI1, MI2, O2, and IO4) stained with ZMJ214 (A, B, E and F) or YoPro1 (C, D, G or H) in response to troglitazone (A and C), pioglitazone (B and D), tolcapone (E and G) or entacapone (F and H) are shown.
