THE UNIVERSITY of EDINBURGH
Centre for Reproductive Health
SuRF@QMRI Further information Find out how the QMRI Small University Facilities can help your research within the QMRI, University, NHS and wider research community.
CALM
(Confocal and Advanced Light Microscopy) Specialising in live cell and whole small organism imaging, Rolly provides expert advice, experimental design, training and general consultancy on light microscopy and related topics. With expertise in a wide range of imaging techniques such as time lapse imaging, image deconvolution, image data analysis and quantitation, the CALM facility provides high-end light microscopy platforms for multidimensional imaging. Rolly also provides twice a year a highly respected course and workshop about the basics of optics and light microscopy by subscription. Imaging-related clinics and workshops are available on request. Practical assistance is also given by the facility manager Shonna Johnston, who at the same time is an expert on flow cytometry. Advanced imaging techniques such as total internal reflection fluorescence (TIRF) microscopy and fluorescence lifetime measurements (FLIM) are not yet available in the CALM facility, but can be offered off-site as collaborations. Rolly also provides general consultancy on microscope infrastructure and the operation of imaging facilities. CALM (Confocal and Advanced Light Microscopy) Head of CALM Facility and Academic Lead: Dr Rolly Wiegand, PhD Telephone 0131 650 2864 Email
[email protected] Facility Manager: Shonna Johnston Telephone 0131 242 9162 Email
[email protected] www.calm.ed.ac.uk
Small University Research Facilities The University of Edinburgh The Queen’s Medical Research Institute 47 Little France Crescent Edinburgh EH16 4TJ
General enquiries
[email protected] Biomolecular Core Facility Manager: Dr Pam Brown PhD, Cert Management Telephone 0131 242 9154 Email
[email protected] Histology Facility Manager: Mr Bob Morris, M.Sc, FIMLS Telephone 0131 242 9200 / 0131 242 9155 Email
[email protected] Immunodetection and Imaging Facility Manager: Mr Mike Millar, MSc, Cert Management Telephone 0131 242 9155 Email
[email protected] CALM (Confocal and Advanced Light Microscopy) Head of CALM Facility and Academic Lead: Dr Rolly Wiegand, PhD Telephone 0131 650 2864 Email
[email protected] Facility Manager: Shonna Johnston Telephone 0131 242 9162 Email
[email protected] The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336.
www.crh.ed.ac.uk/surf
www.crh.ed.ac.uk/surf
The Queen’s Medical Research Institute Small University Research Facilities (SuRF) offer a comprehensive range of services to underpin and support research within the Queen’s Medical Research Institute (QMRI), University, NHS and wider research community.
With a shared ethos of collaboration and integrated activites these SuRF’s provide a comprehensive and complimentary range of support, services and equipment to deliver the results for your cell and tissue based research.
Biomolecular Core
Histology
Immunodetection and Imaging
Biomolecular core facility offers comprehensive production of custom adenoviral and lentiviral vectors, with proven expertise, evidenced by publications and years of experience. Pam can advise users what cargo delivery vectors will be suitable for their experimental system, since adenovirus is episomal, while lentivirus integrates into the genome. Pam has developed a cohort of viral vectors, carrying a range of fluorescent proteins, capable of delivering cargo to manipulate gene expression and fluorescently mark cells in vitro and in vivo.
Delivering high quality tissue processing, paraffin sectioning, cryostat sectioning and special empirical staining techniques.
Delivering high quality immunohistochemical and immunofluorescent staining on cells and tissue sections, specialising in multi immunoflourescent colocalisation staining technologies.
Biomolecular Core can design your viral vector construct or can use your preferred construct, with your gene of interest, to produce high quality viral vectors that can revolutionize your research.
Capable of handling high volume, quick turn around tissue based studies to accredited standards.
Bob runs several histology labs across Edinburgh including an accredited forensic pathology service. Bob and his highly experienced team provide high quality tissue processing, sectioning and staining within a wellequipped lab with automated slide printing, tissue processing and staining machines to keep costs for end users as low as possible.
A full range of secondary antibodies and detection systems are available for purchase in ul quantities. There are currently 1900 antibodies recorded on our database.
Biomolecular Core is also equipped with bookable equipment accessed via http://med-000999.mvm.ed.ac.uk/ including Li-Cor, 4 x ABI 7900, FLA5100 and 2 x fluorescent plate readers.
Facility Manager: Dr Pam Brown PhD, Cert Management Telephone 0131 242 9154 Email
[email protected] www.crh.ed.ac.uk/surf
Equipped with three Vision Biosystems Bond staining robots Mike offers high throughput or custom antibody optimisation services. Time consuming multi colocalisation including same species primaries, are automated saving time and money. A large range of imaging platforms including three confocals, widefield, stereology, image analysis, digital photograpy and PALM laser capture microscopy are available.
Immunodetection and Imaging is also equipped with bookable equipment accessed via http://med-000999.mvm.ed.ac.uk/.
Facility Manager: Mr Bob Morris, M.Sc, FIMLS Telephone 0131 242 9200 / 0131 242 9155 Email
[email protected] www.crh.ed.ac.uk/surf
Facility Manager: Mr Mike Millar, MSc, Cert Management Telephone 0131 242 9155 Email
[email protected] www.crh.ed.ac.uk/surf