Background Hypotheses Methods Results
MicroRNA-146b attenuates myocardial NF-κB signaling following ischemia-reperfusion – potential roles for perioperative cardioprotection Kevin A Friede BA,1 Michael P Smith MS,1 Zhiquan Zhang PhD, 1 Qing Ma MD,1 Kyle KL Phua MS,2 Kam W Leong PhD,2 Mihai V Podgoreanu MD1 (1)Systems Modeling of Perioperative Cardiovascular Injury Laboratory, Dept. of Anesthesiology; (2) Dept. of Biomedical Engineering, Duke University
Methods
Results
• Discovery of dysregulated miRs: miR expression analysis was conducted in myocardial tissues (LV and RA) from two animal models of surgical I/R using GeneChip miRNA arrays (Affymetrix) – a) male rats undergoing cardioplegic arrest (CA), or deep hypothermic circulatory arrest (DHCA) followed by 24h of reperfusion, or sham; and b) male pigs undergoing CA, or DHCA followed by 3h or 6h of reperfusion, or sham. Significantly deregulated miRs (false discovery rate
Methods
Results
• Discovery of dysregulated miRs: miR expression analysis was conducted in myocardial tissues (LV and RA) from two animal models of surgical I/R using GeneChip miRNA arrays (Affymetrix) – a) male rats undergoing cardioplegic arrest (CA), or deep hypothermic circulatory arrest (DHCA) followed by 24h of reperfusion, or sham; and b) male pigs undergoing CA, or DHCA followed by 3h or 6h of reperfusion, or sham. Significantly deregulated miRs (false discovery rate
