Drinking Water Scheme

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Summary of Results External Quality Assessment of Water Microbiology

Drinking Water Scheme Distribution Number: W174

Sample Numbers: W174A, W174B, W174C

Distribution Date: Results Due: Report Date:

March 2017 14 April 2017 25 April 2017

Samples prepared and quality control tested by:

Angela Appea Richard Borrill Thomas Harper Zak Prior Judith Spellar Aneta Stranc Lili Tsegaye

Data analysed by:

Manchari Rajkumar Nita Patel

Report compiled by:

Manchari Rajkumar Nita Patel

Authorised by:

Nita Patel

This report must not be reproduced without permission of the organisers. Public Health England Food and Environmental Proficiency Testing Unit (FEPTU) 61 Colindale Avenue London NW9 5EQ Tel: +44 (0)20 8327 7119 Fax: +44 (0)20 8200 8264 Email: [email protected] The data in FEPTU reports is confidential

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For further information on the scheme please refer to: Scheme Guide: https://www.gov.uk/government/publications/food-and-water-proficiency-testing-schemes-scheme-guide Guide to Scoring and Statistics: https://www.gov.uk/government/publications/food-and-water-proficiency-testing-schemes-scoring-systems-and-statistics

General guidance for z-scores: Participants' enumeration results are converted into z-scores using the following formula:

Z = (xi-Xpt) σpt

xi Xpt

= participants' result expressed (expressed as a log 10 value) = assigned value (participants' consensus median (expressed as a log 10 value))

σpt

= the fixed standard deviation for the examination (calculated by FEPTU)

The σpt-value expresses the acceptable difference between the individual participant 's result and the participants' consensus median. The σpt-value used for calculating z-scores for all parameters in the Drinking Water Scheme is 0.35. A guide to interpreting z-scores follows, although laboratories must interpret their scores in the context of their own laboratory situation. z = -1.99 to +1.99 z = -2 to -2.99 or +2 to +2.99 z= < -3.00 or > + 3.00

satisfactory questionable unsatisfactory

It is usually recommended that z-scores exceeding +/-2 are investigated to establish the possible cause. rule, PHE recommends that all questionable and unsatisfactory results are investigated.

As a general

FEPTU Quality Control: To demonstrate homogeneity of the sample, a minimum of 10 LENTICULE® discs, selected randomly from a batch, are tested in duplicate for parameters requiring enumeration.

To demonstrate stability of the sample, a minimum of nine LENTICULE discs, selected randomly from a batch, are examined throughout the distribution period for enumeration parameters. PHE uses methods stipulated in the DWI 's series of documents: The Microbiology of Drinking Water (2002) - Methods for the Examination of Waters and Associated Materials. The FEPTU results are used for guidance in the preliminary intended results notification, letters are posted on the website immediately after every distribution; electronic notification of their availability is sent to all participants. Refer to section 17.0 of the Scheme Guide if you have experienced difficulties with any of the examinations . https://www.gov.uk/government/publications/food-and-water-proficiency-testing-schemes-scheme-guide

Participants are reminded that reporting an incorrect or false negative results could have serious public health implications. Participants who report unexpectedly high or low results for enumerations on single occasions only should not be unduly alarmed, although they should still assess the reason(s) for the outlying result. The results, as summarised in the performance assessment sheet included in this report, provide a more effective indication of on-going problems with the enumeration of low levels of indicator organisms in drinking water samples. Performance assessment cannot be undertaken as effectively if laboratories do not participate in all distributions for the Drinking Water Scheme.

Please contact FEPTU staff for advice and information: Repeat samples

Carmen Gomes or Kermin Daruwalla

Data Analysis

Manchari Rajkumar or Nita Patel

Microbiological advice

Nita Patel or Zak Prior

General comments and complaints

Nita Patel or Zak Prior

Scheme consultants

Julie E. Russell

Scheme Co-ordinator

Nita Patel

Tel: Fax: Email:

+44 (0)20 8327 7119 +44 (0)20 8200 8264 [email protected]

FEPTU's website

Accreditation: PHE Water EQA Scheme for Drinking Water is accredited by the United Kingdom Accreditation Service (UKAS) to ISO/IEC 17043:2010.

0006 The data in FEPTU reports is confidential

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Sample: W174A Contents:

Klebsiella pneumoniae 16 (wild strain), Escherichia coli 10 (wild strain), Clostridium perfringens 66 (wild strain), Kocuria kristinae 1.2x10² (NCTC 11038)

Expected Results: All counts are expressed as colony forming units (cfu) per 100mL except the colony counts at 37ºC/48h and 22ºC/72h which are cfu per mL.

Colony count 22ºC/72h

Colony count 37ºC/48h

C.perfringens

P.aeruginosa

26

10

0

0

66

121

124

No. results returned

125

131

114

96

92

115

110

Assigned value

27

11

0

0

70

120

116

Uncertainty of assigned value*

0.04

0.03

N/A

N/A

0.02

0.01

0.01

Participants mean (all results)

25

11

0

0

67

119

115

8 - 84

1 - 35

N/A

N/A

22 - 221

38 - 379

37 - 365

0.34

0.32

N/A

N/A

0.16

0.09

0.09

No of outlying counts

23

11

N/A

N/A

5

7

7

False positives

N/A

N/A

0

3

N/A

N/A

N/A

False negatives

2

2

N/A

N/A

1

1

0

E.coli

FEPTU median

Parameter

Enterococci

Coliform bacteria

The fixed standard deviation value (σpt value) used for calculation of the z-scores is 0.35 for all parameters.

(Participants median all results)

Expected Range

Standard deviation** ( log₁₀)

Your result

Score for performance assessment

Z-score

Analyst 2 Result

Analyst 2 Z-score

Analyst 3 Result

Analyst 3 Z-score

*

U(Xpt) is based on results transformed to a log₁₀ scale

**

Robust S* based on median absolute deviation about the participants' median (MADe) and is based on logged data

Total sent samples

144

Not examined

5

Non returns

8

Late returns

0

The data in FEPTU reports is confidential

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Printed at 7:42 on Wednesday, 26 April, 2017

Sample: W174B Contents:

Klebsiella oxytoca 6 (wild strain), Escherichia coli 17 (wild strain), Pseudomonas aeruginosa 37 (wild strain), Staphylococcus xylosus 3 (NCIMB 13636)

Expected Results: All counts are expressed as colony forming units (cfu) per 100mL except the colony counts at 37ºC/48h and 22ºC/72h which are cfu per mL.

Colony count 37ºC/48h

Colony count 22ºC/72h

17

0

37

0

3

3

No. results returned

125

130

114

94

94

114

109

Assigned value

25

20

0

22

0

2

2

Uncertainty of assigned value*

0.03

0.03

N/A

0.04

N/A

0.05

0.05

Participants mean (all results)

24

19

0

23

0

3

3

8 - 79

6 - 63

N/A

7 - 70

N/A

0-6

0-8

0.27

0.27

N/A

0.29

N/A

0.33

0.36

No of outlying counts

22

20

N/A

15

N/A

11

11

False positives

N/A

N/A

1

N/A

2

N/A

N/A

False negatives

2

2

N/A

4

N/A

0

0

E.coli

Parameter

P.aeruginosa

23

Enterococci

FEPTU median

Coliform bacteria

C.perfringens

The fixed standard deviation value (σpt value) used for calculation of the z-scores is 0.35 for all parameters.

(Participants median all results)

Expected Range

Standard deviation** ( log₁₀)

Your result

Score for performance assessment

Z-score

Analyst 2 Result

Analyst 2 Z-score

Analyst 3 Result

Analyst 3 Z-score

*

U(Xpt) is based on results transformed to a log₁₀ scale

**

Robust S* based on median absolute deviation about the participants' median (MADe) and is based on logged data

Total sent samples

144

Not examined

6

Non returns

8

Late returns

0

The data in FEPTU reports is confidential

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Sample: W174C Contents:

Enterococcus faecalis 36 (wild strain), Pseudomonas aeruginosa 70 (wild strain), Clostridium perfringens 48 (wild strain), Staphylococcus epidermidis 5 (wild strain)

Expected Results: All counts are expressed as colony forming units (cfu) per 100mL except the colony counts at 37ºC/48h and 22ºC/72h which are cfu per mL.

Colony count 37ºC/48h

Colony count 22ºC/72h

70

48

5

2

126

132

115

97

93

116

112

0

0

49

55

55

5

1

Uncertainty of assigned value*

N/A

N/A

0.01

0.02

0.02

0.04

0.05

Participants mean (all results)

0

0

48

54

52

5

0

Expected Range

N/A

N/A

15 - 153

17 - 174

17 - 174

1 - 16

0-5

Standard deviation**

N/A

N/A

0.1

0.18

0.19

0.33

0.45

N/A

N/A

10

6

6

9

10

False positives

3

1

N/A

N/A

N/A

N/A

N/A

False negatives

N/A

N/A

1

4

0

0

0

No. results returned

Assigned value

C.perfringens

36

P.aeruginosa

0

FEPTU median

Enterococci

E.coli

0

Parameter

Coliform bacteria

The fixed standard deviation value (σpt value) used for calculation of the z-scores is 0.35 for all parameters.

(Participants median all results)

( log₁₀) No of outlying counts

Your result

Score for performance assessment

Z-score

Analyst 2 Result

Analyst 2 Z-score

Analyst 3 Result

Analyst 3 Z-score

*

U(Xpt) is based on results transformed to a log₁₀ scale

**

Robust S* based on median absolute deviation about the participants' median (MADe) and is based on logged data

Total sent samples

144

Not examined

4

Non returns

8

Late returns

0

The data in FEPTU reports is confidential

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Printed at 7:42 on Wednesday, 26 April, 2017

27 Participant Median 26 FEPTU Median

0

Outlying results

Outlying results

5-7

25

1

2

>106

Outlying results

30

Outlying results

1

PHE score

84-106

0

106

84-106

75-84

70-74

65-69

60-64

55-59

50-54

45-49

40-44

36-39

32-35

28-31

24-27

20-23

16-19

12-15

0

8-11

No. of Results

25

Outlying results

30

45

11 Participant Median 10 FEPTU Median

0

Outlying results

25

1

2

Outlying results

30

35-45

PHE score

No. of Results

20

15

10

5

32-35

30-31

28-29

26-27

24-25

22-23

20-21

18-19

16-17

14-15

12-13

10-11

8-9

6-7

4-5

3-3

45

35-45

32-35

30-31

28-29

26-27

24-25

22-23

20-21

18-19

16-17

14-15

12-13

10-11

8-9

6-7

4-5

3-3

1-2

0

221

200-221

189-199

178-188

167-177

156-166

145-155

134-144

123-133

113-122

103-112

93-102

83-92

73-82

63-72

53-62

43-52

33-42

221

200-221

189-199

178-188

167-177

156-166

145-155

Count per 100mL TSC (Membrane Filtration)

134-144

123-133

113-122

103-112

93-102

83-92

73-82

63-72

53-62

43-52

33-42

22-32

0

379

344-379

326-343

308-325

290-307

272-289

254-271

236-253

218-235

200-217

182-199

164-181

146-163

128-145

110-127

92-109

74-91

56-73

38-55

379

344-379

326-343

308-325

290-307

272-289

254-271

Count per mL

236-253

218-235

200-217

182-199

164-181

146-163

128-145

110-127

92-109

74-91

56-73

38-55

0

365

330-365

312-329

294-311

276-293

259-275

242-258

225-241

208-224

191-207

174-190

157-173

140-156

123-139

106-122

89-105

72-88

55-71

37-54

365

330-365

312-329

294-311

276-293

259-275

242-258

Count per mL

225-241

208-224

191-207

174-190

157-173

140-156

123-139

106-122

89-105

72-88

55-71

37-54

0

100

Outlying results

30

Outlying results

1

PHE score

79-100

0

100

79-100

70-79

65-69

60-64

56-59

52-55

48-51

44-47

40-43

36-39

32-35

28-31

24-27

20-23

16-19

12-15

0

8-11

No. of Results

25

Outlying results

30

78

Outlying results

30

Outlying results

1

PHE score

63-78

0

78

63-78

56-63

52-55

48-51

44-47

40-43

37-39

34-36

31-33

28-30

25-27

22-24

19-21

16-18

13-15

10-12

0

6-9

No. of Results

25

Outlying results

30

76

22 Participant Median 37 FEPTU Median

0

Outlying results

2

70-76

1

Outlying results

No. of Results

25

0

6-6

30

Outlying results

PHE score

76

70-76

63-70

59-62

55-58

51-54

47-50

43-46

39-42

35-38

31-34

27-30

23-26

19-22

16-18

13-15

10-12

0

7-9

No. of Results

25

Outlying results

30

153

138-153

130-137

122-129

114-121

106-113

99-105

92-98

85-91

78-84

71-77

64-70

57-63

50-56

43-49

36-42

29-35

22-28

153

138-153

130-137

122-129

114-121

106-113

Count per 100mL

99-105

92-98

85-91

78-84

71-77

64-70

57-63

50-56

43-49

36-42

29-35

22-28

15-21

0

174

157-174

148-156

139-147

130-138

121-129

113-120

105-112

97-104

89-96

81-88

73-80

65-72

57-64

49-56

41-48

33-40

25-32

174

157-174

148-156

139-147

130-138

121-129

113-120

Count per 100mL Pseudalert (MPN)

105-112

97-104

89-96

81-88

73-80

65-72

57-64

49-56

41-48

33-40

25-32

17-24

0

174

157-174

148-156

139-147

130-138

121-129

112-120

Count per 100mL TSC (Membrane Filtration)

103-111

94-102

85-93

76-84

67-75

59-66

51-58

43-50

35-42

27-34

0

17-26

No. of Results

25

Outlying results

30