HLA Twin Workshop ASHI 2016 Beginners
Agenda §
Introduction to
and to (15 mins)
§
Hands on workflows in HLA Twin (1 h15 mins) § HLA Twin Orientation § Interprete 2 different sampes § Good result § Challenging Result
§ Create a PDF report §
Questions - just put your hand up – we’ll be right over!
Introduc.on to Holotype HLA™ Holotype HLA is a combination Assay and Software product for the comprehensive gene amplification of multiple HLA loci, and sequencing on Illumina sequencing platforms.
NGS Assay + NGS So+ware
Omixon Holotype HLA (Assay) Kits Kit sizes Loci Indexes Samples per run* CE-IVD
5-locus
7-locus
11-locus
96
24 and 96
24 and 96
ABC, DQB1, DRB1
5+ DPB1, DQA1
7+ DRB3/4/5 DPA1
3x 96 index sets
2x 96 index sets 2x 24 index sets
2x 96 index sets 2x 24 index sets
32-288
4-192
4-96
N/A
Q1, 2016
Q1, 2017
Fully pooled (per-sample indexing) MiniSeq, MiSeq and NextSeq supported Nano, Micro & Standard Flow Cells supported 300-cycle and 500-cycle chemistry supported Up to 2 genotyping algorithms
Omixon HLA Twin™ (So?ware) §
So+ware component of Holotype HLA
§
Automated genotyping a+er MiSeq run
§
Preconfigured Holotype HLA-‐specific seAngs
§
Two algorithms for determining HLA genotypes – Consensus Genotyping (Assembly) – StaHsHcal Genotyping (Alignment to IMGT/HLA)
§
Traffic light system for data interpretaHon and workflow management Passed Info Inspect InvesHgate Failed
The Traffic Light System
Implementa.on HLA Twin
HLA Typing Dashboard
Quick Guide NavigaHon buPons
HLA Typing Dashboard
Paired file selecHon
Sample file Analysis file
Ini.a.ng and Viewing an Analysis
PDF Report Genera.on
PDF Report
HLA Typing Analysis Result
HLA Typing Analysis Result
HLA Typing Sample Results
Quality Control
Data Sta.s.cs
Genome Browser
Genome Browser
3 Hands-‐on Workflows in Twin § HLA Twin OrientaHon § Interprete 2 different sampes § Good result § Challenging result
§ Create a PDF report
MyHolotype @ the Omixon Website
Introduction to
and to (15 mins)
§
Hands on workflows in HLA Twin (1 h15 mins) § HLA Twin Orientation § Interprete 2 different sampes § Good result § Challenging Result
§ Create a PDF report §
Questions - just put your hand up – we’ll be right over!
Introduc.on to Holotype HLA™ Holotype HLA is a combination Assay and Software product for the comprehensive gene amplification of multiple HLA loci, and sequencing on Illumina sequencing platforms.
NGS Assay + NGS So+ware
Omixon Holotype HLA (Assay) Kits Kit sizes Loci Indexes Samples per run* CE-IVD
5-locus
7-locus
11-locus
96
24 and 96
24 and 96
ABC, DQB1, DRB1
5+ DPB1, DQA1
7+ DRB3/4/5 DPA1
3x 96 index sets
2x 96 index sets 2x 24 index sets
2x 96 index sets 2x 24 index sets
32-288
4-192
4-96
N/A
Q1, 2016
Q1, 2017
Fully pooled (per-sample indexing) MiniSeq, MiSeq and NextSeq supported Nano, Micro & Standard Flow Cells supported 300-cycle and 500-cycle chemistry supported Up to 2 genotyping algorithms
Omixon HLA Twin™ (So?ware) §
So+ware component of Holotype HLA
§
Automated genotyping a+er MiSeq run
§
Preconfigured Holotype HLA-‐specific seAngs
§
Two algorithms for determining HLA genotypes – Consensus Genotyping (Assembly) – StaHsHcal Genotyping (Alignment to IMGT/HLA)
§
Traffic light system for data interpretaHon and workflow management Passed Info Inspect InvesHgate Failed
The Traffic Light System
Implementa.on HLA Twin
HLA Typing Dashboard
Quick Guide NavigaHon buPons
HLA Typing Dashboard
Paired file selecHon
Sample file Analysis file
Ini.a.ng and Viewing an Analysis
PDF Report Genera.on
PDF Report
HLA Typing Analysis Result
HLA Typing Analysis Result
HLA Typing Sample Results
Quality Control
Data Sta.s.cs
Genome Browser
Genome Browser
3 Hands-‐on Workflows in Twin § HLA Twin OrientaHon § Interprete 2 different sampes § Good result § Challenging result
§ Create a PDF report
MyHolotype @ the Omixon Website
