HLA Twin Workshop EFI 2016 beginners
Agenda §
Introduc)on to
and to (15 mins)
§
Hands on workflows in HLA Twin (45 mins) § HLA Twin Orienta)on § Interprete 2 different sampes § Good result § Challenging Result
§ Create a PDF report §
Ques)ons - just put your hand up – we’ll be right over!
Introduc.on to Holotype HLA™ Holotype HLA is a combina)on Assay and SoSware product for the comprehensive gene amplifica)on of mul)ple HLA loci, and sequencing on the Illumina MiSeq.
NGS Assay + NGS SoSware
Omixon Holotype HLA – up to 11 loci (Assay)
11 loci – HLA-A, B, C, DRB1, DRB3/4/5, DPA1, DPB1, DQA1 and DQB1
§
24 samples
§
96 samples
§
24 Indexes
§
96 Indexes
§
8-24 samples per run
§
32-96 samples per run
Fully pooled (per-sample indexing) 2 genotyping algorithms
Omixon HLA Twin™ (SoEware) §
SoSware component of Holotype HLA
§
Automated genotyping aSer MiSeq run
§
Preconfigured Holotype HLA-specific secngs
§
Two algorithms for determining HLA genotypes – Consensus Genotyping (Assembly) – Sta)s)cal Genotyping (Alignment to IMGT/HLA)
§
Traffic light system for data interpreta)on and workflow management Passed Info Inspect Inves)gate Failed
The Traffic Light System
Implementa.on HLA Twin
HLA Typing Dashboard
Quick Guide Naviga)on bueons
HLA Typing Dashboard
Paired file selec)on
Sample file Analysis file
Ini.a.ng and Viewing an Analysis
PDF Report Genera.on
PDF Report
HLA Typing Analysis Result
HLA Typing Analysis Result
HLA Typing Sample Results
Quality Control
Data Sta.s.cs
Genome Browser
Genome Browser
3 Hands-on Workflows in Twin § HLA Twin Orienta)on § Interprete 2 different sampes § Good result § Challenging result
§ Create a PDF report
Introduc)on to
and to (15 mins)
§
Hands on workflows in HLA Twin (45 mins) § HLA Twin Orienta)on § Interprete 2 different sampes § Good result § Challenging Result
§ Create a PDF report §
Ques)ons - just put your hand up – we’ll be right over!
Introduc.on to Holotype HLA™ Holotype HLA is a combina)on Assay and SoSware product for the comprehensive gene amplifica)on of mul)ple HLA loci, and sequencing on the Illumina MiSeq.
NGS Assay + NGS SoSware
Omixon Holotype HLA – up to 11 loci (Assay)
11 loci – HLA-A, B, C, DRB1, DRB3/4/5, DPA1, DPB1, DQA1 and DQB1
§
24 samples
§
96 samples
§
24 Indexes
§
96 Indexes
§
8-24 samples per run
§
32-96 samples per run
Fully pooled (per-sample indexing) 2 genotyping algorithms
Omixon HLA Twin™ (SoEware) §
SoSware component of Holotype HLA
§
Automated genotyping aSer MiSeq run
§
Preconfigured Holotype HLA-specific secngs
§
Two algorithms for determining HLA genotypes – Consensus Genotyping (Assembly) – Sta)s)cal Genotyping (Alignment to IMGT/HLA)
§
Traffic light system for data interpreta)on and workflow management Passed Info Inspect Inves)gate Failed
The Traffic Light System
Implementa.on HLA Twin
HLA Typing Dashboard
Quick Guide Naviga)on bueons
HLA Typing Dashboard
Paired file selec)on
Sample file Analysis file
Ini.a.ng and Viewing an Analysis
PDF Report Genera.on
PDF Report
HLA Typing Analysis Result
HLA Typing Analysis Result
HLA Typing Sample Results
Quality Control
Data Sta.s.cs
Genome Browser
Genome Browser
3 Hands-on Workflows in Twin § HLA Twin Orienta)on § Interprete 2 different sampes § Good result § Challenging result
§ Create a PDF report
