Quiz Submissions Quiz 2
Quiz Submissions Quiz 2 Attempt 1 Written: Oct 4, 2015 12:37 Oct 4, 2015 13:13
Submission View Question 1
1 / 1 point
Base excision repair of a base pair formed between G and deaminated methylC involves a specific DNA glycosylase that hydrolyzes the covalent bond between a G base and the sugarphosphate backbone a specific DNA glycosylase that hydrolyzes the covalent bond between a T base and the sugarphosphate backbone a specific apurinic endonuclease that hydrolyzes the covalent bonds between G and U bases a specific apyrimidinic endonuclease that hydrolyzes the covalent bonds between G and T bases specific DNA glycosylases that hydrolyze the covalent bonds between G and U bases and the sugarphosphate backbones Question 2 In a standard PCR protocol, which is the correct order of steps in each cycle? a. annealing of the primers b. denaturation of the doublestranded DNA template c. extension by taq DNA polymerase d. removal of the primer by FEN1 c, a, b, d a, b, c
1 / 1 point
c, b, a, d a, c , b, d b, a, c
Question 3
1 / 1 point
Use the figures to answer the following question. In order to clone gene "X" (black rectangle) from the SV40 genome that was digested (i.e. "cut") to completion with the restriction endonucleases EcoR1, Sph1 and Pst1, the plasmid vector must also be digested with EcoR1 and Sph1 Pst1 and Sph1 EcoR1 only EcoR1, Pst1 and Sph1
EcoR1 and Pst1
Question 4
1 / 1 point
Which of the following does not involve gap repair? insertion (i.e. "paste") of a DNA transposon base excision repair nucleotide excision repair proofreading by DNA polymerase delta two of the above Question 5
1 / 1 point
Which is true for microsatellites? repeat units are approx. 14 to 100 bp in length arrays are composed of tandem repeat units expansion of repeat units can occur by backward slippage during DNA replication are often found in telomeres and centromeres two of the above Question 6
1 / 1 point
In addition to a DNA polymerase activity, DNA polymerase alpha has which other enzymatic activity? endonuclease RNA polymerase 3' to 5' exonuclease 5' to 3' exonuclease none of the above Question 7
1 / 1 point
Processed pseudogenes typically lack which of the following? promoter cisregulatory module introns >1 kb introns
Submission View Question 1
1 / 1 point
Base excision repair of a base pair formed between G and deaminated methylC involves a specific DNA glycosylase that hydrolyzes the covalent bond between a G base and the sugarphosphate backbone a specific DNA glycosylase that hydrolyzes the covalent bond between a T base and the sugarphosphate backbone a specific apurinic endonuclease that hydrolyzes the covalent bonds between G and U bases a specific apyrimidinic endonuclease that hydrolyzes the covalent bonds between G and T bases specific DNA glycosylases that hydrolyze the covalent bonds between G and U bases and the sugarphosphate backbones Question 2 In a standard PCR protocol, which is the correct order of steps in each cycle? a. annealing of the primers b. denaturation of the doublestranded DNA template c. extension by taq DNA polymerase d. removal of the primer by FEN1 c, a, b, d a, b, c
1 / 1 point
c, b, a, d a, c , b, d b, a, c
Question 3
1 / 1 point
Use the figures to answer the following question. In order to clone gene "X" (black rectangle) from the SV40 genome that was digested (i.e. "cut") to completion with the restriction endonucleases EcoR1, Sph1 and Pst1, the plasmid vector must also be digested with EcoR1 and Sph1 Pst1 and Sph1 EcoR1 only EcoR1, Pst1 and Sph1
EcoR1 and Pst1
Question 4
1 / 1 point
Which of the following does not involve gap repair? insertion (i.e. "paste") of a DNA transposon base excision repair nucleotide excision repair proofreading by DNA polymerase delta two of the above Question 5
1 / 1 point
Which is true for microsatellites? repeat units are approx. 14 to 100 bp in length arrays are composed of tandem repeat units expansion of repeat units can occur by backward slippage during DNA replication are often found in telomeres and centromeres two of the above Question 6
1 / 1 point
In addition to a DNA polymerase activity, DNA polymerase alpha has which other enzymatic activity? endonuclease RNA polymerase 3' to 5' exonuclease 5' to 3' exonuclease none of the above Question 7
1 / 1 point
Processed pseudogenes typically lack which of the following? promoter cisregulatory module introns >1 kb introns
